FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1671419
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATGGGTAGTGGCATTAAGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC138560.8289962002346509No Hit
CCCATATACAGTACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTC95180.5694562524417874No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC69530.4159938351783724No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTTAATGCCACTACCCATATACAGTACA53040.31733515055171685No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG50820.3040530232096201No Hit
GTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAG50540.3023777999412475No Hit
GATTATTACTGTGTACTGTATATGGGTAGTGGCATTAAGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT47960.2869418141112432No Hit
CTCATCTACAGCACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCCTCCTTGGGAACAAAG46980.2810785326719393No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC45360.27138616947635513No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG43550.2605570476343753No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT43420.25977926540263097No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT43120.2579843833293746No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTTAATGCCACTACCCATATACAGTACACAGTAATAAT42670.25529206021949014No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG42600.254873254402397No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA42290.25301854292669884No Hit
GTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACTCTCACTTGTGGCTTGAGCTCTGG41600.24889031415820925No Hit
CTACTAGTCACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAAACA38480.23022354059634356No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG37680.22543718840099342No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT37530.22453974736436527No Hit
CCTTAATGCCACTACCCATATACAGTACACAGTAATAATCAGATTCATCA37080.22184742425448079No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC36970.22118930082762012No Hit
GTCTCTACTAGTCACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGC36910.22083032441296888No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT36660.21933458935192193No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG35810.21424909014436236No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC35790.2141294313394786No Hit
GGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCAT35770.21400977253459488No Hit
GTACTGTATATGGGTAGTGGCATTAAGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG34600.2070097324488952No Hit
GTAGATGAGCGTGCGTGGAGCCTGGCCTGGGGTCTGCTGGTACCAGCTGG34220.2047362151561039No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG32840.19647975761912484No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT32620.19516351076540353No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG31710.1897190351431927No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC31610.18912074111877392No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC31190.1866079062162151No Hit
ACCCATATACAGTACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTT30560.18283865386237683No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG30190.18062496597202737No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA30100.18008650135005047No Hit
GCATTAAGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC29970.17930871911830606No Hit
CCCCAGAAGAGCGAGTGTTTGTGCTGTAGATGAGCGTGCGTGGAGCCTGG29950.1791890603134223No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG29510.1765565666059797No Hit
CTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCCTCCTTGGGAACAAAGCTGCCCTCACCATCACGGGGGCCCAGG29470.1763172489962122No Hit
CCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAAACACTCGCTCT29420.1760181019840028No Hit
ATACAGTACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGA28970.17332577887411835No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC27840.16656505639818622No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG26660.1595051869100447No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGG26510.15860774587341656No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA25710.15382139367806635No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC25120.15029145893399562No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA24910.14903504148271618No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC24770.1481974298485299No Hit
AGTAGAGACTGAGCCAGAGCTCAAGCCACAAGTGAGAGTGACTGTCCCTCCAGGGGACACTGAGAACGATGGCTC24390.14592391255573856No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT24330.14556493614108731No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC24290.14532561853131978No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACT24060.14394954227515663No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA24050.14388971287271474No Hit
CATCTACAGCACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCCTCCTTGGGAACAAAGCTGC23800.1423939778116678No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC23740.14203500139701655No Hit
GATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACT23460.14035977812864397No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23390.13994097231155084No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT22950.13730847860410825No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA22410.13407769087224686No Hit
ACTGTGTACTGTATATGGGTAGTGGCATTAAGGTGTTCGGCGGAGGGACC22260.1331802498356187No Hit
CCCCTGGAGGGACAGTCACTCTCACTTGTGGCTTGAGCTCTGGCTCAGTC22190.13276144401852558No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT21750.13012895031108299No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC21270.12725713899387286No Hit
GACTAGTAGAGACTGAGCCAGAGCTCAAGCCACAAGTGAGAGTGACTGTC20960.12540242751817468No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT20730.12402635126201152No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20550.12294942201805771No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG20440.12229129859119706No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC20310.12151351635945266No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT20140.12049641651794074No Hit
CTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATT19910.11912034026177758No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTTAATGCCACTAC19670.11768443460317252No Hit
TTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAG19630.11744511699340501No Hit
GGCCTGGGGTCTGCTGGTACCAGCTGGGGTAGTGACTAGTAGAGACTGAG19350.11576989372503244No Hit
GGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTC19180.11475279388352053No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG19110.11433398806642739No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT17930.10727411857828588No Hit
GTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGG17770.10631684813921582No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA17590.10523991889526206No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC17500.10470145427328514No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC17380.10398350144398263No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG17270.10332537801712198No Hit
GCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTCCCAAGGAGGGAGCC17030.10188947235851692No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG17020.10182964295607504No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT16970.10153049594386566No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT16890.10105186072433064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3050.063.0786570
CGTATGC3450.050.14197541
TCGTATG3500.049.3461340
ATCTCGT3100.048.7996137
GTGGTAT4100.047.3409461
GGTATCA15400.047.098251
ATCGTAT306.318506E-445.9907639
TCTCGTA3300.045.9044838
GCCGTCT3800.045.8695646
AATATCG306.449847E-445.79915236
TGCCGTC3800.045.7755945
CTTATAC3750.045.4030081
ATGCCGT3850.045.13141644
AATCTCG3300.044.75826636
TGGTATC4350.044.6028442
GTATGCC3900.044.42796742
GCAATCT3250.044.29633734
ACGAGAC3850.044.21444722
CACGAGA3850.044.21312321
TATACAC4050.043.704413
Produced by FastQC (version 0.11.5)