FastQCFastQC Report
Wed 8 May 2024
EGAF00004691617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870392
Total Bases64.5 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGCTACCACACCGAGGACGTTCGGCCAAGGGA52700.6054743150212778No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT42780.4915026792525667No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38810.44589104679270947No Hit
CAGTAATACACTGCAAAGTCCTCAGGCTCCAGTCTGGTGATGGTGAGAGT33790.3882158843371722No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC30680.3524848573975864No Hit
GTGTATTACTGTCAGCAGTATGCTACCACACCGAGGACGTTCGGCCAAGG27950.3211196794088181No Hit
CTCCTGCACGGCCAGTCAGACTGTTTACAGCAACTACTTAGCCTGGCACC27730.31859208264781846No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26730.30710300646145644No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26350.3027371575106389No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24740.28423974485059605No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT23820.273669794759143No Hit
GTATGCTACCACACCGAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA23550.2705677441888253No Hit
CATCTATGGTGCATCCAACAGGGCCACTGGCATCCCAGACAGGTTCGGTG23450.26941883657018906No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23300.26769547514223474No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG23090.26528276914309873No Hit
GTAATAGACAGCCGTGTCGTCAGTCCTCAGGCTGTTCATTTGCAGATACA23090.26528276914309873No Hit
GTTTACAGCAACTACTTAGCCTGGCACCAGCAGAAACCTGGCCAGGCTCC22690.2606871386685539No Hit
CTACCACACCGAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA22630.2599977940973722No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21890.2514958777194643No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC21360.24540666734069247No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA21090.24230461677037474No Hit
GTAATACACTGCAAAGTCCTCAGGCTCCAGTCTGGTGATGGTGAGAGTGA21090.24230461677037474No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19600.22518589325269533No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19490.22392209487219553No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT19180.22036048125442328No Hit
GTGTGGTAGCATACTGCTGACAGTAATACACTGCAAAGTCCTCAGGCTCC19150.22001580896883244No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC18840.2164541953510602No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18680.21461594316124227No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT18380.2111692203053337No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTGCCAGGC17840.2049651191646982No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC17730.2037013207841984No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA16600.19071866469360932No Hit
CACTTCACTCTCACCATCACCAGACTGGAGCCTGAGGACTTTGCAGTGTA16500.18956975707497312No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC16190.1860081434572009No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCC16000.18382521898179213No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC15950.18325076517247402No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15940.18313587441061038No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14920.17141701670052115No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC14730.16923409222511238No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG14580.16751073079715806No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCACGGCCAGTCAGA14320.16452357098870393No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACC14050.1614215204183862No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT13740.15785990680061399No Hit
GAGTGAAGTGTGTCCCAGACCCACTGCCACCGAACCTGTCTGGGATGCCA13640.15671099918197776No Hit
GTCTATTACTGTGCGAGAGAGGGGGGGTCTGTTTATTATGCTTATAGCAG13630.15659610842011415No Hit
GTAGCATACTGCTGACAGTAATACACTGCAAAGTCCTCAGGCTCCAGTCT13520.15533231003961434No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT12980.14912820889897885No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA12940.14866864585152437No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC12890.14809419204220628No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC12530.14395812461511595No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACC12480.14338367080579786No Hit
GTCTGGGACACACTTCACTCTCACCATCACCAGACTGGAGCCTGAGGACT12450.143038998520207No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12290.14120074633038907No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12230.14051140175920734No Hit
ACCCTCTCCTGCACGGCCAGTCAGACTGTTTACAGCAACTACTTAGCCTG12100.13901782185498027No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAACA11870.13637533433211702No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT11770.1352264267134808No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT11750.13499664518975357No Hit
GTCCCAGACCCACTGCCACCGAACCTGTCTGGGATGCCAGTGGCCCTGTT11720.13465197290416273No Hit
CAACAGGGCCACTGGCATCCCAGACAGGTTCGGTGGCAGTGGGTCTGGGA11480.13189459461943584No Hit
TCTATGGTGCATCCAACAGGGCCACTGGCATCCCAGACAGGTTCGGTGGC11480.13189459461943584No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11220.1289074348109817No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG11160.1282180902398No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11130.12787341795420915No Hit
GTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGG10990.12626494728811846No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10940.12569049347880035No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC10740.12339267824152794No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10710.12304800595593711No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC10700.12293311519407348No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10550.12120975376611917No Hit
GCTGTAAACAGTCTGACTGGCCGTGCAGGAGAGGGTGGCTCTTTCCCCTG10530.12097997224239194No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10340.11879704776698315No Hit
CACCGAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTG10060.1155801064348018No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAA9960.11443119881616559No Hit
CTACTTAGCCTGGCACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCA9720.11167382053143872No Hit
CACCATCACCAGACTGGAGCCTGAGGACTTTGCAGTGTATTACTGTCAGC9710.11155892976957509No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9670.1110993667221206No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9550.10972067757975716No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9500.10914622377043907No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9480.10891644224671182No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA9450.10857176996112096No Hit
ATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGTGT9400.10799731615180287No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9350.10742286234248477No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT9340.10730797158062114No Hit
ATGTAAGTGTGCCCGGATTACTTCCAGGATCATCCGTTCTTCCGAAGATC9340.10730797158062114No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9220.10592928243825771No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9190.10558461015266685No Hit
CCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGT9170.1053548286289396No Hit
CCTCATCTATGGTGCATCCAACAGGGCCACTGGCATCCCAGACAGGTTCG9120.10478037481962149No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC8960.10294212262980358No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT8950.10282723186793997No Hit
GGTTTCTGCTGGTGCCAGGCTAAGTAGTTGCTGTAAACAGTCTGACTGGC8940.10271234110607634No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8900.10225277805862186No Hit
GTGTAGCACTTGTTTCTGTCATAAGTGTAGCAGGTCTCTGTAGCACTGTC8900.10225277805862186No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8880.10202299653489463No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT8830.10144854272557652No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG8800.10110387043998567No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTG8780.10087408891625842No Hit
GAGCCACCCTCTCCTGCACGGCCAGTCAGACTGTTTACAGCAACTACTTA8770.10075919815439481No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8760.10064430739253118No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)