FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870392
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGCTACCACACCGAGGACGTTCGGCCAAGGGA46800.5376887655217419No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40090.46059706431125286No Hit
CAGTAATACACTGCAAAGTCCTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTGTGTCCCAGACCCACTGCCA28190.32387705769354497No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27610.317213393505455No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26350.3027371575106389No Hit
GTGTATTACTGTCAGCAGTATGCTACCACACCGAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA26230.30135846836827546No Hit
CTCCTGCACGGCCAGTCAGACTGTTTACAGCAACTACTTAGCCTGGCACC25940.2980266362742305No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA25520.2932012242759584No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23620.2713719795218706No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23190.26643167676173496No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC21010.24138549067546575No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20680.2375940955339663No Hit
GTATGCTACCACACCGAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT20380.23414737267805769No Hit
GTGTGGTAGCATACTGCTGACAGTAATACACTGCAAAGTCCTCAGGCTCC19920.22886239763233118No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19840.22794327153742222No Hit
GTTTACAGCAACTACTTAGCCTGGCACCAGCAGAAACCTGGCCAGGCTCC19380.22265829649169572No Hit
CATCTATGGTGCATCCAACAGGGCCACTGGCATCCCAGACAGGTTCGGTGGCAGTGGGTCTGGGACACACTTCA19220.2208200443018778No Hit
CTACCACACCGAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA19150.22001580896883244No Hit
GTAATAGACAGCCGTGTCGTCAGTCCTCAGGCTGTTCATTTGCAGATACA18750.21542017849428766No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCACGGCCAGTCAGACTGTTTACAG18730.21519039697056036No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17520.20128861478506235No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17480.2008290517376079No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17310.19887590878592634No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17290.1986461272621991No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17220.19784189192915375No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17160.19715254735797205No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16820.19324626145460896No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC16630.1910633369792002No Hit
GTAATACACTGCAAAGTCCTCAGGCTCCAGTCTGGTGATGGTGAGAGTGA16620.19094844621733656No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15800.1815274037445197No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC15160.17417439498524803No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14930.17153190746238475No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14810.1701532183200213No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14800.1700383275581577No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTGCCAGGCTAAGTAGTTGCTGTAAACAGTCTGA14650.16831496613020341No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14420.16567247860734016No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCACGGCCAGTCAGA14300.1642937894649767No Hit
CACTTCACTCTCACCATCACCAGACTGGAGCCTGAGGACTTTGCAGTGTATTACTGTCAGCAGTATGCTACCACA13500.15510252851588707No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13410.1540685116591145No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC13300.1528047132786147No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12970.14901331813711524No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT12460.1431538892820706No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC12450.143038998520207No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG12340.14177520013970718No Hit
GAGTGAAGTGTGTCCCAGACCCACTGCCACCGAACCTGTCTGGGATGCCA11990.13775402347448046No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11860.1362604435702534No Hit
GTCTATTACTGTGCGAGAGAGGGGGGGTCTGTTTATTATGCTTATAGCAGTGAGGGTTACCTTGATTATTGGGGC11710.13453708214229912No Hit
GTCTGGGACACACTTCACTCTCACCATCACCAGACTGGAGCCTGAGGACT11630.13361795604739016No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC11600.13327328376179928No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC11560.1328137207143448No Hit
GTAGCATACTGCTGACAGTAATACACTGCAAAGTCCTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTGTGTC11520.13235415766689032No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11480.13189459461943584No Hit
ACCCTCTCCTGCACGGCCAGTCAGACTGTTTACAGCAACTACTTAGCCTGGCACCAGCAGAAACCTGGCCAGGCT11410.1310903592863905No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11070.1271840733830274No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11060.1270691826211638No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10650.12235866138475537No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10480.12040551843307383No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10360.11902682929071039No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10200.11718857710089248No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10180.11695879557716522No Hit
GTCCCAGACCCACTGCCACCGAACCTGTCTGGGATGCCAGTGGCCCTGTT10110.11615456024411988No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT9920.11397163576871111No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9850.11316740043566577No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT9660.11098447596025698No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAACAGGGCCACTGGCATCCCAGACAGGTT9530.10949089605602992No Hit
GCTGTAAACAGTCTGACTGGCCGTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGGAGA9150.10512504710521237No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9140.10501015634334875No Hit
GTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGG9140.10501015634334875No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAA9100.10455059329589426No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8870.101908105773031No Hit
GGTTTCTGCTGGTGCCAGGCTAAGTAGTTGCTGTAAACAGTCTGACTGGCCGTGCAGGAGAGGGTGGCTCTTTCC8830.10144854272557652No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8750.10052941663066756No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8710.10006985358321308No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)