FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004691623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418158
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC16990.4063057504579608No Hit
ACTTATTATTGTGCAGCATGGGATGACAGCCTGAGAGCTTGGCTGTTCGG16150.3862176497878792No Hit
CTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTG13860.3314536610563471No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12950.30969155199709203No Hit
GCCCCCACACTCCTCATCTATCGGAATAGTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT12720.30419123871837916No Hit
CTCCTCATCTATCGGAATAGTCAGCGGCCCTCAGGGGTCCCTGACCGATT11280.26975449471252494No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACAGCCAAGCTCTCAGGCTGTCATCCCATG11250.2690370625457363No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG10950.26186274087785No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT10820.25875386815509926No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC10620.25397098704317506No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10490.2508621143204244No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA10250.2451226569861153No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC9960.23818747937382523No Hit
ATAATAAGTAGCCTCATCTTCGAATCGGAGTCCACTGATGGCCAGGGAGG9590.22933914931676544No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT9480.22670856470520712No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9000.21522965003658903No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG8940.21379478570301177No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACAGCCAAGCTCTCAGG8830.21116420109145348No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACGTCAGCC8420.20135929481200887No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8160.1951415493665074No Hit
GCATGGGATGACAGCCTGAGAGCTTGGCTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGG8110.19394582908852634No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG7880.18844551580981353No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT7750.18533664308706277No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC7270.1738577284184447No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT7220.17266200814046367No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCCCCCTCAGCGTCTGGGACCC7120.17027056758450154No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7080.16931399136211672No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG6810.16285710186101904No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC6720.16070480536065315No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA6670.15950908508267211No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC6610.15807422074909486No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACGGCTC6540.15640021235992135No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA6470.1547262039707479No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC6180.1477910263584578No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG6160.1473127382472654No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC6150.14707359419166918No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT6130.14659530608047674No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG5940.14205156902414875No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT5920.14157328091295635No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC5690.1360729676342435No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA5670.1355946795230511No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT5640.13487724735626247No Hit
GCACTGAACACAGAGGACCCACCATGGAGTTTGGGCTGAGCTGGGTTTTC5610.13415981518947384No Hit
TCAGTGGACTCCGATTCGAAGATGAGGCTACTTATTATTGTGCAGCATGG5550.13272495085589658No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG5550.13272495085589658No Hit
GTGTGGGGGCCGTTCCGGGGAACTGCTGGTACCAATATATATAATTATAT5490.1312900865223193No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC5480.1310509424667231No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC5400.1291377900219534No Hit
GTCCATGTAGTGGTCACTGAAGATGAATCCAGAGGCTGCACAGGAGAGTC5390.12889864596635722No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA5360.1281812137995686No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC5330.12746378163277997No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG5240.12531148513241405No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCCCCCTCAGCGTCT5160.12339833268764439No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG5130.12268090052085574No Hit
CTCCTGTGCAGCCTCTGGATTCATCTTCAGTGACCACTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGG5090.12172432429847092No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA5020.12005031590929743No Hit
GGATGACAGCCTGAGAGCTTGGCTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC4980.11909373968691261No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC4840.11574572290856566No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT4800.11478914668618082No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCCC4780.1143108585749884No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT4730.11311513829700735No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC4680.11191941801902631No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA4660.11144112990783388No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATGGCAGCC4620.11048455368544904No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACGTCAG4590.10976712151866042No Hit
GATGAGGAGTGTGGGGGCCGTTCCGGGGAACTGCTGGTACCAATATATAT4550.10881054529627557No Hit
ATCCCATGCTGCACAATAATAAGTAGCCTCATCTTCGAATCGGAGTCCAC4540.10857140124067936No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT4530.10833225718508314No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG4530.10833225718508314No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC4440.10617996068471726No Hit
GTGCTGACTCAGCCCCCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCTC4430.10594081662912105No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA4400.10522338446233243No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG4390.1049842404067362No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT4380.10474509635113999No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC4370.1045059522955438No Hit
GGCCATCAGTGGACTCCGATTCGAAGATGAGGCTACTTATTATTGTGCAG4350.10402766418435139No Hit
ATCGGAATAGTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACGTCAGCCTCCCTGG4340.10378852012875517No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACAATGACCAGGGTTCCCTGGCCCCAGTAGTCAGGGAACGAATAG4340.10378852012875517No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG4330.10354937607315895No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACAGCCAAGCTCTCAGGC4310.10307108796196653No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT4290.10259279985077412No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG4240.10139707957279305No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC4230.10115793551719685No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT4200.10044050335040822No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)