FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418158
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC17070.40821890290273055No Hit
ACTTATTATTGTGCAGCATGGGATGACAGCCTGAGAGCTTGGCTGTTCGG15770.3771301756752232No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14740.3524983379488136No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG12500.2989300694952626No Hit
CTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTG12190.29151660377178007No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG11390.27238507932408323No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC10920.26114530871106134No Hit
GCCCCCACACTCCTCATCTATCGGAATAGTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT10870.25994958843308036No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACAGCCAAGCTCTCAGGCTGTCATCCCATG10550.25229697865400164No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT10480.2506229702648281No Hit
CTCCTCATCTATCGGAATAGTCAGCGGCCCTCAGGGGTCCCTGACCGATT10350.2475140975420774No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9570.228860861205573No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC9530.22790428498318818No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9510.22742599687199574No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG9340.2233605479268602No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA9090.21738194653695495No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT9030.21594708220337766No Hit
ATAATAAGTAGCCTCATCTTCGAATCGGAGTCCACTGATGGCCAGGGAGG8400.20088100670081643No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7740.18509749903146658No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACAGCCAAGCTCTCAGG7590.1815103381975234No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACGTCAGCC7240.17314029625165608No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG7070.1690748473065205No Hit
GCATGGGATGACAGCCTGAGAGCTTGGCTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGG7040.16835741513973188No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT6920.16548768647257733No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCCCCCTCAGCGTCTGGGACCC6890.16477025430578873No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA6880.1645311102501925No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC6840.16357453402780767No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC6760.16166138158303797No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA6630.15855250886028727No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACGGCTC6600.15783507669349864No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG6480.1549653480263441No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC6420.15353048369276687No Hit
GTGTGGGGGCCGTTCCGGGGAACTGCTGGTACCAATATATATAATTATAT6360.15209561935918958No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC6210.14850845852524644No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC6120.14635616202488053No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG6080.1453995858024957No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC5920.14157328091295635No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT5890.14085584874616772No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG5720.13679039980103214No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA5680.1358338235786473No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC5550.13272495085589658No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT5550.13272495085589658No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG5400.1291377900219534No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACAG5400.1291377900219534No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA5350.12794206974397238No Hit
CTCCTGTGCAGCCTCTGGATTCATCTTCAGTGACCACTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGG5260.1257897732436065No Hit
TCAGTGGACTCCGATTCGAAGATGAGGCTACTTATTATTGTGCAGCATGG5230.12507234107681786No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA5200.12435490891002923No Hit
GTCCATGTAGTGGTCACTGAAGATGAATCCAGAGGCTGCACAGGAGAGTC5170.1236374767432406No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCCCCCTCAGCGTCT5170.1236374767432406No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG5160.12339833268764439No Hit
GGATGACAGCCTGAGAGCTTGGCTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC5160.12339833268764439No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG5120.12244175646525955No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA5090.12172432429847092No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCCC4980.11909373968691261No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT4930.11789801940893156No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC4840.11574572290856566No Hit
GCACTGAACACAGAGGACCCACCATGGAGTTTGGGCTGAGCTGGGTTTTC4760.113832570463796No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC4740.11335428235260356No Hit
GATGAGGAGTGTGGGGGCCGTTCCGGGGAACTGCTGGTACCAATATATAT4720.11287599424141113No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC4640.11096284179664145No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG4580.1095279774630642No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT4560.10904968935187179No Hit
ATCGGAATAGTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACGTCAGCCTCCCTGG4530.10833225718508314No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG4530.10833225718508314No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC4520.10809311312948694No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACGTCAG4410.10546252851792862No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA4390.1049842404067362No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG4360.10426680823994758No Hit
GTGCTGACTCAGCCCCCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCTC4330.10354937607315895No Hit
GGCCATCAGTGGACTCCGATTCGAAGATGAGGCTACTTATTATTGTGCAG4320.10331023201756274No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCC150.00211191270.91746555
ACTTCGT200.006509156453.37471857
TCGAGCA200.006999908552.40283648
GTGCTAT306.8069354E-445.293066
CTATCTT405.4746277E-542.4622424
TATCTTC350.001453362638.822625
TGTCCCT350.001453362638.8226210
GGTATCA3350.038.5790521
GTATCAA8250.037.9268341
TGGTATC1200.036.8138242
GCTAAGG400.00205533836.1770364
GAACGGT4850.035.71621370
GTGGTAT1250.035.37091
AATGGTA2057.166818E-1032.49974470
GCCAGAG4100.032.49974470
TATCAAC9700.032.230342
GTTTTAC553.5378392E-430.918621
TAGTAGT553.5627582E-430.881634
ATCAACG10300.030.3455813
ACGGGAC450.004977032530.20259716
Produced by FastQC (version 0.11.5)