FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004691625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences722234
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT55730.7716335702833154No Hit
CTATTAAGCTGTTGACAGGAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC52580.728018896922604No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46810.6481278920682216No Hit
GCTTAATAGTTACCCTTATACTTTTGGCCAGGGGACCAAGCTGGAGATCCGACGAACTGTGGCTGCACCAT46270.6406510909206712No Hit
GTATAAGGGTAACTATTAAGCTGTTGACAGGAATAAGTTGCAAAATCTTC36140.5003918397638438No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34700.4804537033703758No Hit
CCCTTATACTTTTGGCCAGGGGACCAAGCTGGAGATCCGACGAACTGTGG33900.4693769609295602No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33720.46688469388037673No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGACGTTAGCAGATTTTTAGCCTGGTATCAGCAAAAA31290.4332390887163994No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30700.4250699911662979No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30430.4213315905925227No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29340.4062395290169114No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27570.38173223636660697No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC25930.359024914362935No Hit
GATCTATGCTACATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA25560.3539019209840578No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24910.34490206775089516No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24020.3325791917854878No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG23910.3310561396998757No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23790.3293946283337533No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23590.32662544272354943No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23480.32510239063793733No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23460.3248254720769169No Hit
GTGCAGCCACAGTTCGTCGGATCTCCAGCTTGGTCCCCTGGCCAAAAGTA23310.32274858286926394No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22920.31734867092936636No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22360.30959495122079544No Hit
ATGCTACATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCA22210.3075180620131426No Hit
GTCAGGACGTTAGCAGATTTTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTGACCTCCTGATCTATGCTA21560.2985182087799799No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTCCTGTCAACAGCTTAATAGTTAC20910.2895183555468172No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCAT20740.28716454777814393No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20300.2810723394356954No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20070.27788777598396086No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19630.2717955676415123No Hit
GTCTCCCTCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT19280.2669494928236555No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG18490.2560112096633501No Hit
CCCTCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG18430.25518045398028893No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17090.23662691039192285No Hit
GATCAGGAGGTCAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAAA16940.2345500211842699No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16690.23108853917151506No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16640.2303962427689641No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16140.22347327874345435No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15780.21848874464508733No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCATCCACTTGACCCAGTCTCCCTCCTTC15730.21779644824253636No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC15640.21655031471794461No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15140.20962735069243485No Hit
CTCCTGATCTATGCTACATCCAATTTGCAAAGTGGGGTCCCATCAAGGTT15120.2093504321314145No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTG14990.20755046148478196No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14690.20339668306947611No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14450.20007366033723142No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14130.1956429633609052No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT13820.19135072566508915No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCGGATCTCCAGCTTGGTCC13740.1902430514210076No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13580.18802770293284446No Hit
GTGCCAGATGTGCCATCCACTTGACCCAGTCTCCCTCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA13490.18678156940825272No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13450.18622773228621195No Hit
ACTTATTCCTGTCAACAGCTTAATAGTTACCCTTATACTTTTGGCCAGGGGACCAAGCTGGAGATCCGACGAA13410.18567389516417118No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13300.18415084307855903No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13130.18179703530988572No Hit
GCATAGATCAGGAGGTCAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGC12830.17764325689457988No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12650.17515098984539637No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12590.1743202341623352No Hit
GGGTAACTATTAAGCTGTTGACAGGAATAAGTTGCAAAATCTTCAGGCTG12550.17376639704029442No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12510.17321255991825366No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12310.17044337430804976No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12300.17030491502753956No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12140.16808956653937643No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12100.16753572941733566No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12090.16739727013682545No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12070.16712035157580507No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12060.1669818922952949No Hit
ACCTTGATGGGACCCCACTTTGCAAATTGGATGTAGCATAGATCAGGAGGTCAGGGGCTTTCCCTGGTTTTTG12010.1662895958927439No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11860.16421270668509097No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11690.1618588989164177No Hit
GGCCAAAAGTATAAGGGTAACTATTAAGCTGTTGACAGGAATAAGTTGCA11400.15784357978162203No Hit
CACCATCACTTGCCGGGCCAGTCAGGACGTTAGCAGATTTTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCC11340.15701282409856085No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC11330.15687436481805064No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11260.1559051498544793No Hit
GCCCCTGACCTCCTGATCTATGCTACATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGAT11120.15396671992733657No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC10990.15216674928070403No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10790.14939756367050014No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10650.1474591337433574No Hit
CTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTCCTGTCA10620.14704375590182683No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10610.14690529662131663No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10530.1457976223772351No Hit
GGAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG10470.1449668666941739No Hit
GAGTCACCATCACTTGCCGGGCCAGTCAGGACGTTAGCAGATTTTTAGCCTGGTATCAGCAAAAACCAGGGAAAG10420.14427457029162294No Hit
GAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCT10410.14413611101111273No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCCGACGAACTGTGGCTGCACCA10370.14358227388907197No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10300.1426130589255006No Hit
ATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATT10270.14219768108397002No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC10210.14136692540090884No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT10200.14122846612039866No Hit
TAGTTACCCTTATACTTTTGGCCAGGGGACCAAGCTGGAGATCCGACGAACTGTGGCTGCACCATCTGTCTTCAT10190.14109000683988845No Hit
ATGTAGCATAGATCAGGAGGTCAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAAAATCTGCTAACGTC10140.14039771043733748No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10110.1399823325958069No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9980.13818236194917435No Hit
CACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTCCTGTCAAC9970.13804390266866418No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG9900.13707468770509282No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC9490.13139785720417482No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT9400.13015172367958305No Hit
CCTCCTGATCTATGCTACATCCAATTTGCAAAGTGGGGTCCCATCAAGGT9360.12959788655754229No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC9360.12959788655754229No Hit
AGTCAGGACGTTAGCAGATTTTTAGCCTGGTATCAGCAAAAACCAGGGAA9360.12959788655754229No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGACGTTAGC9350.12945942727703208No Hit
GTATCAACGCAGAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT9250.12807483447193013No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9230.12779791591090978No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9200.12738253806937916No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT8920.12350567821509373No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCATCCACTTGACCCAGTC8860.12267492253203256No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8820.12212108540999177No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA8700.12045957404386945No Hit
GCCACAGTTCGTCGGATCTCCAGCTTGGTCCCCTGGCCAAAAGTATAAGG8690.12032111476335924No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT8570.1186596033972369No Hit
CTCCAGCTTGGTCCCCTGGCCAAAAGTATAAGGGTAACTATTAAGCTGTT8370.11589041778703302No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT8290.11478274354295147No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8290.11478274354295147No Hit
GTAATAGACAGCCGTGTCCTCGACTCTCAGGCTGTCCATTTGCAGATACA8270.11450582498193107No Hit
GCTCTGGCTCCCAGGTGCCAGATGTGCCATCCACTTGACCCAGTCTCCCTCCTTCCTGTCTGCATCTGTAGGAGA8020.1110443429691762No Hit
GTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCC8000.1107674244081558No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG7990.1106289651276456No Hit
ACCCAGTCTCCCTCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCAT7970.11035204656662523No Hit
TTTTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTGACCTCCTGAT7970.11035204656662523No Hit
CCCCTGACCTCCTGATCTATGCTACATCCAATTTGCAAAGTGGGGTCCCA7950.11007512800560483No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT7890.10924437232254366No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG7870.10896745376152328No Hit
GGACCCCACTTTGCAAATTGGATGTAGCATAGATCAGGAGGTCAGGGGCTTTCCCTGGTTTTTGCTGATACCAGG7840.10855207591999269No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTCGGATCTCCAGCTTGGTCCCCTGGC7800.10799823879795191No Hit
GGTCAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAAAATCTGCTA7760.10744440167591114No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG7600.10522905318774801No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7530.10425983822417664No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGG7490.10370600110213588No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT7450.10315216398009511No Hit
CATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATG7360.10190603045550334No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG7320.10135219333346256No Hit
CCCTTATACTTTTGGCCAGGGGACCAAGCTGGAGATCCGACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC7270.10065989693091158No Hit
AAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTC7240.100244519089381No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)