FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004691626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences722234
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53990.7475416554745415No Hit
CTATTAAGCTGTTGACAGGAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC50160.6945117510391369No Hit
GCTTAATAGTTACCCTTATACTTTTGGCCAGGGGACCAAGCTGGAGATCCGACGAACTGTGGCTGCACCAT45870.6351127197002634No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39140.5419296239169022No Hit
GTATAAGGGTAACTATTAAGCTGTTGACAGGAATAAGTTGCAAAATCTTC35670.49388425357986476No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34360.4757460878330292No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33690.4664693160388461No Hit
CCCTTATACTTTTGGCCAGGGGACCAAGCTGGAGATCCGACGAACTGTGG33340.46162324122098936No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31220.43226987375282805No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28770.39834735002783034No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGACGTTAGCAGATTTTTAGCCTGGTATCAGCAAAAA27490.3806245621225254No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27390.37923996931742343No Hit
GATCTATGCTACATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA24210.3352099181151815No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23470.3249639313574271No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC23440.3245485535158965No Hit
GTGCAGCCACAGTTCGTCGGATCTCCAGCTTGGTCCCCTGGCCAAAAGTA23250.3219178271862028No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG23100.3198409379785499No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22690.3141641074776319No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22530.3119487589894688No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22360.30959495122079544No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22220.3076565212936528No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22040.30516425424446925No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21340.2954721046087556No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21190.2933952154011027No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21030.29117986691293957No Hit
ATGCTACATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCA20920.2896568148273274No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20690.28647225137559296No Hit
GTCAGGACGTTAGCAGATTTTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTGACCTCCTGATCTATGCTA20240.2802415837526342No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTCCTGTCAACAGCTTAATAGTTAC18780.26002652879814575No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT18520.2564265875048807No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18370.25434969829722776No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18310.2535189426141666No Hit
CCCTCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG17420.24119606664875928No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17190.2380115031970248No Hit
GTCTCCCTCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT17030.23579615470886167No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCAT16800.23261159125712716No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16490.22831935356131114No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16220.2245809529875359No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15780.21848874464508733No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15740.21793490752304656No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15600.21599647759590382No Hit
GATCAGGAGGTCAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAAA15510.21475034407131208No Hit
CTCCTGATCTATGCTACATCCAATTTGCAAAGTGGGGTCCCATCAAGGTT14730.20395052019151685No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14710.20367360163049647No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC14320.1982736896905989No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13990.19370453343376248No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13850.19176610350661974No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13510.18705848796927313No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13370.1851200580421304No Hit
GGGTAACTATTAAGCTGTTGACAGGAATAAGTTGCAAAATCTTCAGGCTG13360.1849815987616202No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCATCCACTTGACCCAGTCTCCCTCCTTC13120.18165857602937552No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTG12920.17888939041917162No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12880.17833555329713086No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12850.17792017545560027No Hit
ACTTATTCCTGTCAACAGCTTAATAGTTACCCTTATACTTTTGGCCAGGGGACCAAGCTGGAGATCCGACGAA12700.17584328624794734No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12510.17321255991825366No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12040.16670497373427448No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12010.1662895958927439No Hit
GCATAGATCAGGAGGTCAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGC11970.16573575877070312No Hit
ACCTTGATGGGACCCCACTTTGCAAATTGGATGTAGCATAGATCAGGAGGTCAGGGGCTTTCCCTGGTTTTTG11940.16532038092917253No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11890.16462808452662156No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11820.1636588695630502No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11760.16282811387998902No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11680.1617204396359075No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11580.16033584683080554No Hit
GTGCCAGATGTGCCATCCACTTGACCCAGTCTCCCTCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA11560.16005892826978513No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11550.15992046898927495No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11500.15922817258672398No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCCGACGAACTGTGGCTGCACCA11260.1559051498544793No Hit
GGCCAAAAGTATAAGGGTAACTATTAAGCTGTTGACAGGAATAAGTTGCA11230.1554897720129487No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11220.15535131273243852No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11050.1529975049637652No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11040.152859045683255No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11010.15244366784172445No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCGGATCTCCAGCTTGGTCC10980.15202829000019383No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10920.15119753431713268No Hit
CACCATCACTTGCCGGGCCAGTCAGGACGTTAGCAGATTTTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCC10900.1509206157561123No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10750.14884372654845937No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10680.147874511584888No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT10550.14607454093825545No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10490.14524378525519432No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC10260.1420592218034598No Hit
GGAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG10190.14109000683988845No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10160.14067462899835786No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10150.1405361697178477No Hit
GCCCCTGACCTCCTGATCTATGCTACATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGAT10130.14025925115682727No Hit
ATGTAGCATAGATCAGGAGGTCAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAAAATCTGCTAACGTC10120.1401207918763171No Hit
CTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTCCTGTCA10030.13887465835172533No Hit
ATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATT10000.13845928051019477No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC9860.13652085058305205No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9830.13610547274152143No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTGCAAATT9830.13610547274152143No Hit
GAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCT9700.13430550209488892No Hit
GTATCAACGCAGAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT9630.13333628713131757No Hit
TAGTTACCCTTATACTTTTGGCCAGGGGACCAAGCTGGAGATCCGACGAACTGTGGCTGCACCATCTGTCTTCAT9610.13305936857029715No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9480.13125939792366464No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT9450.13084402008213403No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9390.13001326439907288No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC9210.12752099734988936No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT9140.126551782386318No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA9110.12613640454478742No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT9100.12599794526427724No Hit
CACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTCCTGTCAAC8990.12447489317866509No Hit
GAGTCACCATCACTTGCCGGGCCAGTCAGGACGTTAGCAGATTTTTAGCCTGGTATCAGCAAAAACCAGGGAAAG8870.12281338181254274No Hit
CCTCCTGATCTATGCTACATCCAATTTGCAAAGTGGGGTCCCATCAAGGT8810.12198262612948159No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG8810.12198262612948159No Hit
CCCTTATACTTTTGGCCAGGGGACCAAGCTGGAGATCCGACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC8790.12170570756846119No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT8720.12073649260488982No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8670.12004419620233885No Hit
AGTCAGGACGTTAGCAGATTTTTAGCCTGGTATCAGCAAAAACCAGGGAA8600.1190749812387675No Hit
GCCACAGTTCGTCGGATCTCCAGCTTGGTCCCCTGGCCAAAAGTATAAGG8590.1189365219582573No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG8500.11769038843366555No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCGGATCTCCAGCTTGGTCCCCTGGCCAAAAGTATAAGGGTAA8320.11519812138448204No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCATCCACTTGACCCAGTC8270.11450582498193107No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC8210.11367506929886989No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG8200.1135366100183597No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG8190.11339815073784949No Hit
GTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCC8180.11325969145733931No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGACGTTAGC8050.11145972081070678No Hit
CCCCTGACCTCCTGATCTATGCTACATCCAATTTGCAAAGTGGGGTCCCA7950.11007512800560483No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT7730.10702902383438054No Hit
GATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATG7700.10661364599284996No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGG7640.1057828903097888No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG7560.10467521606570725No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC7530.10425983822417664No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA7470.10342908254111548No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTCGGATCTCCAGCTTGGTCCCCTGGC7430.10287524541907471No Hit
ACCCAGTCTCCCTCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCAT7380.10218294901652374No Hit
GCTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTG7290.10093681549193198No Hit
TTTTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTGACCTCCTGAT7280.10079835621142179No Hit
GGTCAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAAAATCTGCTA7280.10079835621142179No Hit
GGACCCCACTTTGCAAATTGGATGTAGCATAGATCAGGAGGTCAGGGGCTTTCCCTGGTTTTTGCTGATACCAGG7270.10065989693091158No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7270.10065989693091158No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA7260.10052143765040139No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)