FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712477
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34630.48605077777949324No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28070.3939776301550787No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAAATACA27970.3925740760754382No Hit
CCTATGAGCTGACTCAGCCACCCTCAATGTCCGTGTCCCCAGGACAGACA21310.29909737437138323No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC20970.29432529050060563No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20780.29165853774928874No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20670.29011462826168427No Hit
GTATATTACTGTGCGACCCAGTACCCTAATTGGAGATCTCCCTTTGACTC17760.24927120454414667No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16980.23832348272295106No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGTGCTGCTGTCCCACGCCTGACAGTAATAGTCAG16920.23748135027516679No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16760.23523566374774202No Hit
GTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG16390.2300425136530723No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16350.22948109202121614No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15990.22442829733451045No Hit
ACTATATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAGGAATACTTTGTATTTG15860.22260367703097783No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14210.19944503471691016No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC14040.19705899278152136No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG14040.19705899278152136No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13930.19551508329391687No Hit
CTATTACTGTCAGGCGTGGGACAGCAGCACTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC13090.18372522902493693No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13000.18246203035326053No Hit
CCCTCAATGTCCGTGTCCCCAGGACAGACAGCCAGCCTCACCTGCTCTGG12560.17628639240284247No Hit
GTATATAGTACCACCACTATTTACTGAGACCCACTCCAGCCCCTTCCCTG12480.1751635491391301No Hit
CCCCAGGACAGACAGCCAGCCTCACCTGCTCTGGAGAGAAGTTGGGGCAT12430.17446177209930988No Hit
GACTATTACTGTCAGGCGTGGGACAGCAGCACTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG12400.17404070587541773No Hit
GGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGCAGCACTGTGGTATTCGGCGGAGGGACCAAG12250.17193537475595702No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG12150.1705318206763166No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT11900.1670229354772154No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG11790.1654790259896109No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11450.16070694211883332No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11400.16000516507901308No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11400.16000516507901308No Hit
ATCAAGATACCAAGCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAGCCACTCTGA10960.15382952712859504No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10820.15186455141709837No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10710.15032064192949385No Hit
TCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATC10510.14751353377021292No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10410.1461099796905725No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT10390.14582926887464437No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10320.14484678101889606No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10220.14344322693925557No Hit
GAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCT10050.1410571850038668No Hit
AGCCAGGCCAGTCCCCCGTGCTGGTCATCTATCAAGATACCAAGCGGCCC10010.14049576337201058No Hit
GAATACCACAGTGCTGCTGTCCCACGCCTGACAGTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGT9700.13614474572512517No Hit
GTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGG9690.1360043903171611No Hit
CAGCCACTCTGACCATCAGCGGGACCCAGGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGCAGCA9620.1350219024614128No Hit
CCCCCGTGCTGGTCATCTATCAAGATACCAAGCGGCCCTCAGGGATCCCT9620.1350219024614128No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC9530.13375870378973637No Hit
GGATCCGTGGCCTCCTATGAGCTGACTCAGCCACCCTCAATGTCCGTGTCCCCAGGACAGACAGCCAGCCTCAC9480.13305692674991615No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9430.1323551497100959No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9400.13193408348620375No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9220.12940768614285095No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9210.1292673307348869No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9180.12884626451099473No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC8990.12617951175967784No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8930.12533737931189357No Hit
CAGTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGT8890.1247759576800374No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGG8720.12238991574464861No Hit
GGAGAGAAGTTGGGGCATAAATATGTTTGTTGGTATCAGCAGAAGCCAGGCCAGTCCCCCGTGCTGGTCATCTAT8720.12238991574464861No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT8710.12224956033668455No Hit
GCACAGGATCCGTGGCCTCCTATGAGCTGACTCAGCCACCCTCAATGTCCGTGTCCCCAGGACAGACAGCCAGC8540.11986351840129576No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAG8390.11775818728183506No Hit
CTCCTGTGTAGCCTCTGGACTCACCTTTAGCGACTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG8280.11621427779423055No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8170.11467036830662605No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8150.11438965749069793No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8130.11410894667476985No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC8100.11368788045087772No Hit
GCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGGCCTCCTATGAGCTGACT8080.11340716963494962No Hit
GCTCTGGAGAGAAGTTGGGGCATAAATATGTTTGTTGGTATCAGCAGAAG7940.11144219392345298No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7900.11088077229159678No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT7900.11088077229159678No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC7840.1100386398438125No Hit
GTGCTGCTGTCCCACGCCTGACAGTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGG7700.10807366413231585No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC7580.1063893992367473No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGTGCTGCTGTCCCACGCCTGACAGTAATAGTCAGC7580.1063893992367473No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7410.1040033573013585No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA7380.10358229107746636No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7370.10344193566950231No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA7350.10316122485357422No Hit
GGCCAGTCCCCCGTGCTGGTCATCTATCAAGATACCAAGCGGCCCTCAGG7220.1013366045500416No Hit
CAGCAGCACTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA7200.10105589373411353No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7130.10007340587836519No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGT150.001839539573.43465460
GCGGTAC252.4524253E-455.7101644
AACGGCC252.7113184E-454.58989737
CGCGGTA306.0997694E-446.3168943
GATTGAC452.282306E-644.654351
CGAAAAT359.370719E-442.44976463
ATTAGAG405.9819708E-541.834073
AACGCAT350.001321024139.58770442
TAAGACA350.001564535438.248294
GTATCAA12650.034.946881
CGGTACC400.002452791234.9004145
GTCACCG6200.034.5355370
TAACGCA400.00258775434.5213641
TTAACGG400.002804096833.96071635
TTTAACG400.002841505233.8690434
AGTGCGC400.00300647733.48135813
TAACCGT450.003817001131.88860753
TATCAAC13650.031.8847662
CCGCGGT450.004531247530.79043642
GTTAACG450.004731159730.51991739
Produced by FastQC (version 0.11.5)