FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678062
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT34450.5080656341160543No Hit
CCCCTGTCCTGGTCATCTATCAAGATTCCAAGCGGCCCTCAGGGATCCCT18260.26929690795237016No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17550.2588258890779899No Hit
GCCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACACCACTACTG15570.22962501954098594No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAGACCGCTGCAGTAGTGGTGTCCCACGCCTGA14340.21148508543466527No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13770.20307877450734596No Hit
TCCATAGGCTGGGCCGCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATC13600.2005716291430577No Hit
CCTATGAGCTGACTCAGCCACCCTCGGTGTCCGTGTCCCCAGGACAGACA12860.18965817285145015No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG12350.1821367367585855No Hit
GGACAGGGGACTGGCCTGCCTTCAATTGATACCAGAAAGTATATTTAATT12150.17918715397706994No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG11410.16827369768546238No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAGACCGCTGCAGTAGTGGTGTCCCACGCCTGACAGTAATAGT11260.16606151059932572No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC11250.16591403146024997No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT10840.15986738675814308No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC10560.1557379708640213No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9630.14202241092997395No Hit
CCACTACTGCAGCGGTCTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA9430.1390728281484584No Hit
CTACTGCAGCGGTCTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT9300.13715559934047328No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9120.1345009748371093No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT9070.1337635791417304No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC8960.13214130861189685No Hit
GTCCTGGTCATCTATCAAGATTCCAAGCGGCCCTCAGGGATCCCTGAGCG8950.13199382947282107No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG8870.13081399636021485No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT8780.12948668410853287No Hit
GACTATTACTGTCAGGCGTGGGACACCACTACTGCAGCGGTCTTCGGCGGAGGGACCAAACTGACCGTCCTAGGT8780.12948668410853287No Hit
GGCCAGTCCCCTGTCCTGGTCATCTATCAAGATTCCAAGCGGCCCTCAGG8720.12860180927407816No Hit
CCCCAGGACAGACAGCCACTCTCACCTGCTCTGGACATAGATTGGGAATT8580.1265371013270173No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA8420.12417743510180486No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGG8340.12299760198919862No Hit
CTATTACTGTCAGGCGTGGGACACCACTACTGCAGCGGTCTTCGGCGGAG8270.12196524801566819No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG8190.12078541490306197No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC8100.11945810265137995No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC8060.11886818609507686No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA7910.11665599900894019No Hit
GTAATAGTCAGCCTCATCCATAGGCTGGGCCGCGCTGATGGTCAGAGTGG7740.11414885364465195No Hit
GAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCT7670.11311649967112151No Hit
CAGTAATAGTCAGCCTCATCCATAGGCTGGGCCGCGCTGATGGTCAGAGT7630.1125265831148184No Hit
CAGCCACTCTGACCATCAGCGCGGCCCAGCCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACACCACTA7610.11223162483666685No Hit
CTGCAGTAGTGGTGTCCCACGCCTGACAGTAATAGTCAGCCTCATCCATA7580.11178918741943952No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7500.1106093543068333No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT7480.11031439602868175No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG7450.1098719586114544No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAG7280.10736481324716618No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG7180.10589002185640842No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA7090.1045627096047264No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC6800.10028581457152885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG5650.084.8796570
ATCGCGT200.007696766451.1635537
AACGGGA200.00843616149.9900213
CCGTCTT7000.046.75988847
GCCGTCT7000.046.6001946
CGTATGC7100.046.26040641
AAAGGGG6500.045.56402268
ATCTCGT6700.045.30901737
TCGTATG7200.045.0137240
ACTAGCG7450.044.9060227
CTAGCGC7450.044.52288428
GTATGCC7550.044.5212142
CGTCTTC7250.044.3007848
TAGCGCT7650.044.2709429
GACTAGC7550.044.24572426
ATCTCCG7800.044.00506610
ACGAGAC7900.043.46085722
GCGCTCA7750.043.3764731
AAGGGGG7850.043.320269
CTTATAC7850.043.316241
Produced by FastQC (version 0.11.5)