FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678062
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG71481.0541808861136592No Hit
CCCCTGTCCTGGTCATCTATCAAGATTCCAAGCGGCCCTCAGGGATCCCT17570.2591208473561415No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16110.23758889305107792No Hit
GCCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACACCACTACTG15230.22461072881240945No Hit
TCCATAGGCTGGGCCGCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATC13750.2027838162291944No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAGACCGCTGCAGTAGTGGTGTCCCACGCCTGA13480.19880187947414837No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13100.19319767218926884No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG12480.1840539655665706No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC11340.16724134371193194No Hit
GGACAGGGGACTGGCCTGCCTTCAATTGATACCAGAAAGTATATTTAATT11260.16606151059932572No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAGACCGCTGCAGTAGTGGTGTCCCACGCCTGACAGTAATAGT11230.1656190731820984No Hit
CCTATGAGCTGACTCAGCCACCCTCGGTGTCCGTGTCCCCAGGACAGACA11150.16443924006949218No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10760.15868755364553683No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG10570.15588545000309706No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG10030.14792157649300505No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC9800.14452955629426217No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT9560.1409900569564435No Hit
GTCCTGGTCATCTATCAAGATTCCAAGCGGCCCTCAGGGATCCCTGAGCG9080.13391105828080618No Hit
CTACTGCAGCGGTCTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT8850.1305190380820633No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT8800.1297816423866844No Hit
CCACTACTGCAGCGGTCTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA8740.12889676755222973No Hit
CTATTACTGTCAGGCGTGGGACACCACTACTGCAGCGGTCTTCGGCGGAG8740.12889676755222973No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA8490.1252097890753353No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC8430.12432491424088062No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8280.12211272715474397No Hit
GACTATTACTGTCAGGCGTGGGACACCACTACTGCAGCGGTCTTCGGCGGAGGGACCAAACTGACCGTCCTAGGT8260.1218177688765924No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGG8130.1199005400686073No Hit
GGCCAGTCCCCTGTCCTGGTCATCTATCAAGATTCCAAGCGGCCCTCAGG8130.1199005400686073No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA7980.11768835298247063No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT7790.11488624934003085No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC7580.11178918741943952No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG7570.11164170828036374No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7570.11164170828036374No Hit
GTAATAGTCAGCCTCATCCATAGGCTGGGCCGCGCTGATGGTCAGAGTGG7410.10928204205515131No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG7370.1086921254988482No Hit
CCCCAGGACAGACAGCCACTCTCACCTGCTCTGGACATAGATTGGGAATT7320.1079547298034693No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG7270.1072173341080904No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC7210.10633245927363574No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT7130.10515262616102952No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC7120.10500514702195375No Hit
GAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCT6960.1026454807967413No Hit
CTGCAGTAGTGGTGTCCCACGCCTGACAGTAATAGTCAGCCTCATCCATA6960.1026454807967413No Hit
CAGCCACTCTGACCATCAGCGCGGCCCAGCCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACACCACTA6920.10205556424043818No Hit
GTATCAACGCAGAGTACGGGGGGCCTCAGGAAGCAGCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTT6810.10043329371060464No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC200.0084816249.922091
CTAATGG659.0229674E-442.77079470
GTGGTAT2650.042.7006531
TCTCGTA350.001608459738.0330718
TCGCTAT400.003095935633.281391
TATCAAC23200.031.6940362
AATGGTA4300.030.71042670
TCAACGC25000.030.0641844
CAACGCA25200.029.5616345
TAAAGGG450.005533701729.557286
ATCAACG25300.029.4426213
AACGCAG25750.029.0550886
TCTAATG650.007023425328.14793269
ACGCAGA27100.027.6076977
TCGGAGC657.6543493E-427.10148447
CGCAGAG27650.027.0585378
GTATCAA28650.026.9503751
GTTATAC500.0092152526.625111
CGTGTCT2650.026.48733567
AGAGTAC28150.026.34167711
Produced by FastQC (version 0.11.5)