FastQCFastQC Report
Wed 8 May 2024
EGAF00004691631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2105051
Total Bases153.2 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC107670.5114840448046152No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT102340.48616399317641235No Hit
AGTCAGGACATTGGGACCGCCTTCGCCTGGTATCGCCAGAAACCAGGGCA73830.3507278445985394No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC73660.34992026321452546No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA73190.3476875382116633No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC72050.3422719924600402No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC69800.33158341531867874No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG69540.3303482908490103No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC65520.31125136635644457No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG63810.30312804772900986No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG62220.29557478654911445No Hit
CCCCTAGGCTCCTGATCCATTCTGCATCCACTTTGTGGAGTGGGGTCCCA61140.290444269521261No Hit
GTAATAGAGGGCCGCGTCTGCGGCGGTCACAGAGGTCAGCCTCAGGGAGA60590.2878315062200393No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT59940.2847436950458682No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG53130.25239293489801434No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT52710.2503977338316269No Hit
GAGTGAAGTCTGTCCCAGATCCACTGGCGCTGAATCTTGATGGGACCCCA52430.24906759978736856No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA51660.24540973116565823No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC51090.24270195828984667No Hit
GTTGGAGACAGAGTCACTATCACTTGTCGGGCGAGTCAGGACATTGGGAC50610.24042172849968957No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCT49380.23457863966241196No Hit
GACTTCACTCTCACCATCAACAGCCTGCAGCCTGAGGATTCTGCAACTTA49310.23424610615134742No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT49210.233771058278398No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC48620.23096827582799656No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA48290.22940061784726354No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG48180.22887806518701923No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTG47790.22702537848251655No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT45780.21747691623623372No Hit
GCCTAGGGGCTTGCCCTGGTTTCTGGCGATACCAGGCGAAGGCGGTCCCA44530.21153881782436626No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC43430.2063132912219229No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT43300.20569572898708868No Hit
CAGTAATAGAGGGCCGCGTCTGCGGCGGTCACAGAGGTCAGCCTCAGGGA42580.20227538430185305No Hit
GTCCTGACTCGCCCGACAAGTGATAGTGACTCTGTCTCCAACAGACGCAG40540.19258440769368532No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG40350.1916818167350815No Hit
GTCCCAGATCCACTGGCGCTGAATCTTGATGGGACCCCACTCCACAAAGT37320.17728786618471476No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG37130.17638527522611092No Hit
CATTGGGACCGCCTTCGCCTGGTATCGCCAGAAACCAGGGCAAGCCCCTA37120.176337770438816No Hit
GTTGATGGTGAGAGTGAAGTCTGTCCCAGATCCACTGGCGCTGAATCTTG36320.17253738745522082No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC35700.16959209064293454No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT35270.16754938478925213No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC34960.16607673638310902No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC34950.1660292315958141No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG34900.16579170765933937No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT34500.16389151616754177No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG33660.15990111403476687No Hit
GATCCATTCTGCATCCACTTTGTGGAGTGGGGTCCCATCAAGATTCAGCG33570.1594735709491124No Hit
GTCTCCATCTTCCGTGTCTGCGTCTGTTGGAGACAGAGTCACTATCACTT33510.15918854222534276No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA32810.155863207114697No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT32560.15467558743232349No Hit
CCTTGGTCCCTTGGCCGAACGTGCCAGGAAAACTGCCAGCCTGTTGACAA32480.15429554913396398No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA31970.15187280498192207No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC31760.15087520444872832No Hit
CACCATCAACAGCCTGCAGCCTGAGGATTCTGCAACTTACTATTGTCAAC31760.15087520444872832No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG31690.15054267093766374No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA31520.14973508955364978No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG31160.14802491721103195No Hit
GTCTCCAACAGACGCAGACACGGAAGATGGAGACTGGGTCAGCTGGATGT30910.14683729752865846No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC30910.14683729752865846No Hit
CACTATCACTTGTCGGGCGAGTCAGGACATTGGGACCGCCTTCGCCTGGT30840.14650476401759385No Hit
CTATTACTGTGCGTATGCCCCTAATCGCTACTACCTTGACTACTGGGGCC30450.14465207731309124No Hit
ATCTGGGACAGACTTCACTCTCACCATCAACAGCCTGCAGCCTGAGGATT30280.14384449592907725No Hit
CCACAAAGTGGATGCAGAATGGATCAGGAGCCTAGGGGCTTGCCCTGGTT30150.14322693369424305No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCC29900.14203931401186956No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC29820.14165927571351003No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT29550.14037664645654668No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC29520.14023413209466185No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA29500.14013912252007196No Hit
GTCCCAATGTCCTGACTCGCCCGACAAGTGATAGTGACTCTGTCTCCAAC29040.13795390230450474No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC28990.13771637836803005No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT28750.1365762634729515No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC28520.13548365336516788No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG28390.13486609113033365No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC28350.1346760719811539No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT28300.1344385480446792No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC27830.13220582304181702No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACA27720.1316832703815727No Hit
GGACATTGGGACCGCCTTCGCCTGGTATCGCCAGAAACCAGGGCAAGCCC27670.13144574644509802No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCC27610.13116071772132837No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA27520.1307331746356739No Hit
ATTGTCAACAGGCTGGCAGTTTTCCTGGCACGTTCGGCCAAGGGACCAAG27240.1294030405914156No Hit
GAATGGATCAGGAGCCTAGGGGCTTGCCCTGGTTTCTGGCGATACCAGGC27000.12826292569633704No Hit
CACCTGTACTGTCTCTGGTGACTCCATCAGTCGTGATGATTCCTACTGGA26810.1273603347377332No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA26480.12579267675700018No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC26410.1254601432459356No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT26120.12408250441438237No Hit
GTTGACAATAGTAAGTTGCAGAATCCTCAGGCTGCAGGCTGTTGATGGTG26040.12370246611602283No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT25820.12265736079553417No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA25620.12170726504963539No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG25590.12156475068775055No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA25490.12108970281480115No Hit
CCAGAAACCAGGGCAAGCCCCTAGGCTCCTGATCCATTCTGCATCCACTT25460.12094718845291634No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA25410.12070966451644165No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG25400.1206621597291467No Hit
CTCTATTACTGTGCGTATGCCCCTAATCGCTACTACCTTGACTACTGGGG25260.11999709270701754No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG25230.11985457834513273No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT25040.11895198738652887No Hit
GCCTTCGCCTGGTATCGCCAGAAACCAGGGCAAGCCCCTAGGCTCCTGAT24280.11534162355211347No Hit
CCATCAACAGCCTGCAGCCTGAGGATTCTGCAACTTACTATTGTCAACAG23920.11363145120949564No Hit
CATCAAGATTCAGCGCCAGTGGATCTGGGACAGACTTCACTCTCACCATC23530.111778764504993No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC23230.11035362088614481No Hit
CTCCTGATCCATTCTGCATCCACTTTGTGGAGTGGGGTCCCATCAAGATT23130.10987857301319541No Hit
ATTCTGCATCCACTTTGTGGAGTGGGGTCCCATCAAGATTCAGCGCCAGT23130.10987857301319541No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC23010.10930851556565614No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG22990.10921350599106625No Hit
GATCAGGAGCCTAGGGGCTTGCCCTGGTTTCTGGCGATACCAGGCGAAGG22980.1091660012037713No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT22960.10907099162918144No Hit
GCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTC22950.10902348684188648No Hit
ACTTACTATTGTCAACAGGCTGGCAGTTTTCCTGGCACGTTCGGCCAAGG22940.10897598205459155No Hit
ATCTTGATGGGACCCCACTCCACAAAGTGGATGCAGAATGGATCAGGAGC22900.10878596290541179No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT22880.1086909533308219No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC22680.1077408575849231No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC22570.10721830492467878No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC22480.10679076183902432No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT22420.10650573311525469No Hit
GGTTTCTGGCGATACCAGGCGAAGGCGGTCCCAATGTCCTGACTCGCCCG22140.10517559907099638No Hit
GTGATAGTGACTCTGTCTCCAACAGACGCAGACACGGAAGATGGAGACTG22060.10479556077263687No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCT21810.10360794109026336No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGA21790.1035129315156735No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC21530.10227780704600506No Hit
CTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCCAGCTGACCCAGTC21500.10213529268412025No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21380.10156523523658097No Hit
GGGCAAGCCCCTAGGCTCCTGATCCATTCTGCATCCACTTTGTGGAGTGG21270.10104268257633664No Hit
CTGCATCCACTTTGTGGAGTGGGGTCCCATCAAGATTCAGCGCCAGTGGA21080.10014009161773277No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)