FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2105051
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT90090.42797062874011127No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC79940.3797532696357475No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC66920.31790203657773614No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC66550.3161443594478233No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64460.306215858903181No Hit
AGTCAGGACATTGGGACCGCCTTCGCCTGGTATCGCCAGAAACCAGGGCAAGCCCCTAGGCTCCTGATCCATT61930.2941971477175612No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC57040.27096730673033576No Hit
CCCCTAGGCTCCTGATCCATTCTGCATCCACTTTGTGGAGTGGGGTCCCA54750.2600887104397946No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA54640.2595661577795502No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT53970.25638333703078925No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC52460.2492101141492534No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG51950.2467873699972115No Hit
GTAATAGAGGGCCGCGTCTGCGGCGGTCACAGAGGTCAGCCTCAGGGAGAACTGGTTCTTGGACGTGTCTAGTGA50510.23994668062674016No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTG49170.23358103912921824No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT49050.233010981681679No Hit
GTTGGAGACAGAGTCACTATCACTTGTCGGGCGAGTCAGGACATTGGGACCGCCTTCGCCTGGTATCGCCAGAAA47210.2242701008194101No Hit
GAGTGAAGTCTGTCCCAGATCCACTGGCGCTGAATCTTGATGGGACCCCA47030.22341501464810115No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG45480.21605177261738553No Hit
GACTTCACTCTCACCATCAACAGCCTGCAGCCTGAGGATTCTGCAACTTACTATTGTCAACAGGCTGGCAGTTTT45250.2149591625096019No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT45240.21491165772230697No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC44170.20982864548174845No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT44030.20916357845961928No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG43790.2080234635645407No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT41940.19923507791497688No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG41020.19486463748384242No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT40500.19239438854450558No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG38990.1852211656629697No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG38740.1840335459805962No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTGCCAGGAAAA38310.1819908401269138No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG37350.1774303805465996No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT36950.175530189054802No Hit
GTCCTGACTCGCCCGACAAGTGATAGTGACTCTGTCTCCAACAGACGCAGACACGGAAGATGGAGACTGGGTCAG36690.17429506458513355No Hit
GCCTAGGGGCTTGCCCTGGTTTCTGGCGATACCAGGCGAAGGCGGTCCCA36680.17424755979783862No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA36660.17415255022324874No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT36130.17163479649661695No Hit
CAGTAATAGAGGGCCGCGTCTGCGGCGGTCACAGAGGTCAGCCTCAGGGA35740.1697821097921143No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG33200.15771589381919962No Hit
GTCCCAGATCCACTGGCGCTGAATCTTGATGGGACCCCACTCCACAAAGT31960.15182530019462712No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG31930.15168278583274228No Hit
GTTGATGGTGAGAGTGAAGTCTGTCCCAGATCCACTGGCGCTGAATCTTG31820.15116023317249797No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC30740.14602971614464447No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC30270.1437969911417823No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG29680.14099420869138088No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG29470.13999660815818712No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA29210.1387614836885187No Hit
CACCATCAACAGCCTGCAGCCTGAGGATTCTGCAACTTACTATTGTCAACAGGCTGGCAGTTTTCCTGGCACGTT29110.13828643581556932No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA28960.1375738640061452No Hit
GTCTCCATCTTCCGTGTCTGCGTCTGTTGGAGACAGAGTCACTATCACTTGTCGGGCGAGTCAGGACATTGGGAC28890.13724133049508064No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC28800.13681378740942618No Hit
GATCCATTCTGCATCCACTTTGTGGAGTGGGGTCCCATCAAGATTCAGCGCCAGTGGATCTGGGACAGACTTCA28480.13529363421598814No Hit
CCTTGGTCCCTTGGCCGAACGTGCCAGGAAAACTGCCAGCCTGTTGACAATAGTAAGTTGCAGAATCCTCAGG28390.13486609113033365No Hit
CATTGGGACCGCCTTCGCCTGGTATCGCCAGAAACCAGGGCAAGCCCCTAGGCTCCTGATCCATTCTGCATCCA28230.13410601453361462No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA28200.1339635001717298No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27420.13025812676272452No Hit
GTCTCCAACAGACGCAGACACGGAAGATGGAGACTGGGTCAGCTGGATGTCGCATCTGGAACCTGGGAACCAGAG27170.12907050708035103No Hit
ATCTGGGACAGACTTCACTCTCACCATCAACAGCCTGCAGCCTGAGGATTCTGCAACTTACTATTGTCAACAGG27110.12878547835658138No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA26930.12793039218527247No Hit
CCACAAAGTGGATGCAGAATGGATCAGGAGCCTAGGGGCTTGCCCTGGTTTCTGGCGATACCAGGCGAAGGCGGT26470.12574517196970525No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT26280.12484258101110139No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT25410.12070966451644165No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA25240.11990208313242766No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG25180.11961705440865804No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA25110.11928452089759345No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC24970.11861945387546428No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC24950.11852444430087443No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCCAG24950.11852444430087443No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC24910.11833442515169466No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC24880.11819191078980984No Hit
CTATTACTGTGCGTATGCCCCTAATCGCTACTACCTTGACTACTGGGGCC24860.11809690121521996No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT24840.11800189164063007No Hit
CACTATCACTTGTCGGGCGAGTCAGGACATTGGGACCGCCTTCGCCTGGTATCGCCAGAAACCAGGGCAAGCCC24700.11733682461850094No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC24450.11614920493612745No Hit
ATTGTCAACAGGCTGGCAGTTTTCCTGGCACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTG23910.1135839464222007No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC23850.11329891769843105No Hit
GGACATTGGGACCGCCTTCGCCTGGTATCGCCAGAAACCAGGGCAAGCCCCTAGGCTCCTGATCCATTCTGCATC23770.11291887940007153No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT23340.11087617354638914No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC23030.109403525140246No Hit
GTCCCAATGTCCTGACTCGCCCGACAAGTGATAGTGACTCTGTCTCCAACAGACGCAGACACGGAAGATGGAGA23010.10930851556565614No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC22300.10593567566771542No Hit
CACCTGTACTGTCTCTGGTGACTCCATCAGTCGTGATGATTCCTACTGGA21720.10318039800460892No Hit
CTCTATTACTGTGCGTATGCCCCTAATCGCTACTACCTTGACTACTGGGG21490.10208778789682531No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA21330.10132771130010626No Hit
GTTGACAATAGTAAGTTGCAGAATCCTCAGGCTGCAGGCTGTTGATGGTGAGAGTGAAGTCTGTCCCAGATCCA21250.10094767300174676No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT21230.10085266342715687No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT21200.10071014906527205No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA21170.10056763470338724No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT21080.10014009161773277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12700.038.6328771
GTATCAA34100.038.3685261
GTGGTAT3700.037.887071
AGCGACG400.001724142237.50252563
TATCAAC37250.034.8435332
GCTCGGG7600.034.78045370
CTAGTGA14900.033.49831869
ATCAACG39400.032.930433
TCAACGC39650.032.891824
CAACGCA40750.032.24955
AACGCAG41450.031.5431846
GATTCAC3450.030.95811
AATGGTA9000.030.95773570
CGCAGAG43000.030.4837328
AGAGTAC42800.030.39022311
CTCTTCA14300.029.97521670
CAGAGTA43400.029.9693710
GCAGAGT44150.029.6106389
ACGTTTG500.005913464429.16122857
TACTCCG1053.2392563E-828.5895655
Produced by FastQC (version 0.11.5)