FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219265
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT9830.4483159647002486No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6480.2955328027729004No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6110.2786582445898798No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6080.2772900371696349No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4960.22621029348049165No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4060.18516407087314438No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3900.17786696463183818No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3840.17513054979134837No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3730.17011378925045037No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3610.16464095956947072No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3590.16372882128930746No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3380.1541513693475931No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3100.14138143342530726No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3040.13864501858481745No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2970.135452534604246No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2960.13499646546416436No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2900.13226005062367455No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2790.12724329008277654No Hit
GTATAATAACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT2770.12633115180261326No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2590.11812190728114381No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2430.11082480103983765No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2360.10763231705926618No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2260.10307162565844981No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2230.10170341823820492No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC2220.10124734909812327No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2210.10079127995804163No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2200.10033521081796No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACATA100.0036558362180.8371970
GGAGTTG200.00456864658.35439346
GCGTATA250.00464495858.10439765
TTGAAAA2350.054.5648858
CTTGAAA2350.053.73506557
CGTCTTC2100.053.05600448
CGTATGC2000.052.79102341
CCGTCTT2250.052.392647
GCTTGAA2400.052.15729556
CTCGTAT1950.051.81662839
TCGTATG2000.051.7527540
TAATCTC1850.051.27585635
GATACGC200.009072361549.0656935
ATCTCGT2000.048.7164137
TATGCCG2250.048.43068743
GTAATCT1950.048.21788834
CTTCTGC2400.048.08730751
GTATGCC2250.047.8446642
CTGCTTG2500.047.45415554
ATGCCGT2350.047.0977744
Produced by FastQC (version 0.11.5)