EGAF00004691634 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00004691634
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	219265
Sequences flagged as poor quality	0
Sequence length	20-76
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	1558	0.7105557202471895	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	723	0.3297379882790231	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	703	0.32061660547739035	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	624	0.2845871434109411	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	556	0.2535744418853898	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	429	0.19565366109502202	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	341	0.155519576767838	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	338	0.1541513693475931	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	328	0.14959067794677672	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	326	0.14867853966661346	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	322	0.1468542631062869	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	308	0.140469295145144	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	308	0.140469295145144	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	304	0.13864501858481745	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	287	0.13089184320342964	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	279	0.12724329008277654	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	279	0.12724329008277654	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	279	0.12724329008277654	No Hit
GTATCAACGCAGAGTACGGGTCTGGTGCAACGTGGGGGCTGGAGGGTGGG	272	0.12405080610220509	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	261	0.11903404556130709	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	249	0.11356121588032746	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	240	0.10945659361959273	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	223	0.10170341823820492	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	223	0.10170341823820492	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	220	0.10033521081796	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCAAC	20	0.0025290637	67.74377	62
AAAACGT	25	0.005173059	56.546204	65
TAAACTC	20	0.0069875075	52.415146	39
ACTGACG	25	0.009565978	48.40817	52
GGAATGG	40	1.17970405E-4	37.265266	19
ACTATCG	50	1.0596921E-5	35.693016	11
ACCCCCT	35	0.0027867581	33.993347	12
TGTTAGA	155	1.7196355E-5	33.215145	70
GAATGGT	45	2.3481082E-4	33.147427	20
TTCACTA	45	2.3862484E-4	33.056633	8
GTTCACT	45	2.3862484E-4	33.056633	7
GTCCCCA	60	0.0033266966	32.777317	67
CTATCGG	55	2.0350091E-5	32.448196	12
GGATGAT	40	0.0036966065	32.087395	32
CGCTTCT	50	0.0048754304	30.32021	45
ATCGGTC	60	3.681703E-5	29.750969	14
TATCGGT	60	3.681703E-5	29.750969	13
TTGGACA	40	0.005351995	29.750967	4
CCATCCC	40	0.0053579723	29.74418	10
CGGAACG	55	0.00722001	27.978746	47

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content