FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1442110
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCTGCAGTATGGTAGTTTACCTCGGACGTTCGGCCAAGGGA71720.49732683359799185No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64880.4498963324573021No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAAGCTGCTGATGGTAAGAGTGAAGTCTGTCGCAGACCCACTGCCA60610.4202869406633336No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53050.367863755192045No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAAGCTGCTGATGGTAAGAGTGAAGTCTGTCGCAGACCCACTGCCA48870.3388784489394013No Hit
GTGTATTACTGTCTGCAGTATGGTAGTTTACCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAAACAAACGAA46220.3205025968892803No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38300.2655830692526922No Hit
CTCCTGCACGGCCAGTCAGCCTATTAGCAGCACCCGCTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAG38220.26502832654929237No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36340.2519918730193952No Hit
GTATGGTAGTTTACCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAAACAAACGAACTGTGGCTGCACCAT36090.25025830207127053No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33450.23195179285907455No Hit
GCCTATTAGCAGCACCCGCTTAGCCTGGTACCAACAGAAGCCTGGCCAGG33270.2307036217764248No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33120.22966347920755004No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30670.21267448391592875No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29570.2050467717441804No Hit
CATCTATGCTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGCGACAGACTTCA29510.2046307147166305No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG28140.19513074592090754No Hit
GCTAGGACCCAGAGGGAACCATGGAAACCCCGGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA27970.1939519176761828No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCACGGCCAGTCAGCCTATTAGCAG27600.19138623267295837No Hit
CTACCATACTGCAGACAGTAATACACTGCAAAATCTTCAGGCTCCAAGCT26370.1828570636081852No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25130.1742585517054871No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25060.1737731518400122No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24780.17183155237811262No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24640.17086075264716286No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCACGGCCAGTCAGCCTATTAGCAGCA24600.17058338129546288No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24450.16954323872658814No Hit
GTGCAGCCACAGTTCGTTTGTTTTCCACCTTGGTCCCTTGGCCGAACGTC24070.1669082108854387No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGTTTTCCACCTTGGTCC23680.16420384020636428No Hit
GTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCACGGCCAGTCAGC23460.16267829777201462No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23180.16073669831011506No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22600.15671481371046592No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22520.15616007100706603No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC22360.15505058560026627No Hit
GAGTGAAGTCTGTCGCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA20890.1448571884252935No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT20790.14416376004604364No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20760.1439557315322687No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20290.1406966181497944No Hit
GCATAGATGAGGAGGCTGGGAGCCTGGCCAGGCTTCTGTTGGTACCAGGCTAAGCGGGTGCTGCTAATAGGCTGA19390.13445576273654575No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19310.13390102003314588No Hit
CTGCTACTCTGGCTCCCAGATATCACCGGAGAAATTGTGTTGACGCAGTC19110.13251416327464619No Hit
GCTCAGCTAGGACCCAGAGGGAACCATGGAAACCCCGGCGCAGCTTCTCT18870.13084993516444654No Hit
GTAATAGACGGCCGTGTCGTCAGATTTCAGGCTCATCAGCTCCATGTAGGCTGTGCTCGAGGATGTGTCTGTGGT17960.1245397369132729No Hit
GTGCAGGAGAGGGTGGCTCTCTCCCCTGGAGACAAAGACAGGGTGCCTGG17940.12440105123742294No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17840.12370762285817309No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17440.12093390934117369No Hit
ACCCTCTCCTGCACGGCCAGTCAGCCTATTAGCAGCACCCGCTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCT17400.12065653798947376No Hit
CGCTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGCCTCCTCATCTATGCTGCATCCAGCAGGGCCA17360.12037916663777382No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17250.11961639542059899No Hit
GCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGCCTCCTCATCTATGCTGCATCCAGCAGGGCCACTGGCATC17130.11878428136549916No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16940.11746676744492446No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16560.11483173960377502No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16370.11351422568320031No Hit
ACAGAAGCCTGGCCAGGCTCCCAGCCTCCTCATCTATGCTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTT15760.10928431256977623No Hit
GACCCAGAGGGAACCATGGAAACCCCGGCGCAGCTTCTCTTCCTCCTGCT15760.10928431256977623No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15160.10512374229427714No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15140.10498505661842718No Hit
ATGCTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGCGACAGACTTCACTCTTA15130.10491571378050217No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACC14920.10345951418407749No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14890.10325148567030254No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG14720.10207265742557779No Hit
GCTAATAGGCTGACTGGCCGTGCAGGAGAGGGTGGCTCTCTCCCCTGGAGACAAAGACAGGGTGCCTGGAGACTG14670.10172594323595288No Hit
GGACCAAGGTGGAAAACAAACGAACTGTGGCTGCACCATCTGTCTTCATC14660.1016566003980279No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)