FastQCFastQC Report
Wed 8 May 2024
EGAF00004691643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences813811
Total Bases57.6 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG116001.4253923822607462No Hit
GTATCAACGCAGAGTACGGGTGACCACGGGTGACGGGGAATCAGGGTTCG111511.3702198667749637No Hit
GAGTACGGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAG99801.2263289633588141No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT93741.1518644992510545No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC82101.0088337464104074No Hit
GAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATC56650.6961075728885453No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT49840.6124272097575482No Hit
GGTATCAACGCAGAGTACGGGTGACCACGGGTGACGGGGAATCAGGGTTC44560.5475472806339555No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT43870.5390686535325769No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA43780.5379627456497884No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA40670.4997474843667633No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39300.4829131088176493No Hit
CCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAA37690.4631296455810993No Hit
GGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA37510.4609178298155223No Hit
GGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC35150.4319184675557347No Hit
GTACGGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGG34840.4281092292927965No Hit
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT29370.3608946057499837No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT27710.34049674924521783No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25080.30817966333706476No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT25070.30805678468342157No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG24940.3064593621860604No Hit
CCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGT24590.30215860930854954No Hit
GAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGC24330.29896376431382715No Hit
TATCAACGCAGAGTACGGGTGACCACGGGTGACGGGGAATCAGGGTTCGA23650.29060801586609175No Hit
CCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGA22350.2746337908924799No Hit
GGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACA22270.2736507616633346No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC21070.25890532322615445No Hit
ACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGG20850.2562019928460048No Hit
CCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGG19750.24268534094525634No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT19370.23801595210681598No Hit
ATCAACGCAGAGTACGGGTGACCACGGGTGACGGGGAATCAGGGTTCGAT18290.22474505751335383No Hit
CGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACG17380.21356310003182558No Hit
GACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAG17310.2127029494563234No Hit
CGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA17080.20987674042253052No Hit
GCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGA16840.2069276527350945No Hit
GGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAG16560.20348705043308582No Hit
CGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCG15240.1872670681521877No Hit
ACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCG15160.18628403892304232No Hit
GTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAG15010.18444085911839483No Hit
GGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATT14900.18308919392831996No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAA14790.1817375287382451No Hit
GAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCA14710.18075449950909978No Hit
CACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCC13000.15974224973611809No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG12520.15384407436124603No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCCGTACTCTG11940.14671711244994232No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11450.14069605842142707Clontech SMART CDS Primer II A (100% over 21bp)
TGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCG11150.13700969881213207No Hit
GCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAAT10990.13504364035384137No Hit
GTATCAACGCAGAGTACGGGGACCACGGGTGACGGGGAATCAGGGTTCGA10880.13369197516376652No Hit
GTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCT10410.12791667844253765No Hit
GTATCAACGCAGAGTACGGGTGGTGACCACGGGTGACGGGGAATCAGGGT10340.12705652786703545No Hit
GTATCAACGCAGAGTACGGGACCACGGGTGACGGGGAATCAGGGTTCGAT10020.1231244109504541No Hit
GTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCC9700.11919229403387273No Hit
TCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATG9610.11808638615108422No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA9390.11538305577093452No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAG9190.11292548269807116No Hit
GTGGTATCAACGCAGAGTACGGGTGACCACGGGTGACGGGGAATCAGGGT9070.11145093885435316No Hit
GGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCG8950.10997639501063516No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA8800.10813321520598763No Hit
TCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTC8750.10751882193777179No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8680.10665867136226961No Hit
ATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAG8530.10481549155762211No Hit
GCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTA8430.10358670502119043No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT8410.10334094771390409No Hit
CAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAA8380.10297231175297458No Hit
ACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAA8320.10223503983111558No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)