FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1457145
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37590.2579702088673399No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT9350.038.683631
GGTATCA30300.037.782341
GTATCAA69650.037.2085081
TGGTATC10750.034.2466472
ATCAACG81500.031.6578043
TCAACGC82150.031.3669474
TATCAAC84700.030.6606862
CAACGCA84550.030.5178995
AACGCAG85350.030.3095666
CGCAGAG88000.029.3224648
ACGCAGA88350.029.2053017
AGAGTAC92700.027.87060211
GCAGAGT93150.027.7706139
CAGAGTA93700.027.60665710
TGCTCGA1657.2759576E-1226.12872710
ACGGGTA6900.025.9655416
GAGTACG77150.024.24720212
AGTACGG77600.024.23648513
ATACCGT700.001678485723.6897566
CCTTGCG2203.6379788E-1223.1261654
Produced by FastQC (version 0.11.5)