FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98301
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT8370.8514664143803218No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3750.3814813684499649No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3610.36723939736116623No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3170.3224789167963703No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2620.26652831609037547No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2240.22787153742077904No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2100.21362956633198035No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2070.21057771538438064No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2060.2095604317351807No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2050.20854314808598082No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1990.20243944619078139No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1940.19735302794478185No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1880.1912493260495824No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1690.17192093671478417No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1670.16988636941638438No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1670.16988636941638438No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1620.16479995117038485No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1520.15462711467838577No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1510.15360983102918588No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1500.15259254737998595No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1460.14852341278318634No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1340.13631600899278745No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1290.13122959074678792No Hit
GTATCAACGCAGAGTACGGGTCTGGTGCAACGTGGGGGCTGGAGGGTGGG1270.12919502344838812No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1240.12614317250078838No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1240.12614317250078838No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1230.12512588885158848No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1200.12207403790398877No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1190.12105675425478886No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1180.12003947060558895No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC1170.11902218695638905No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1150.11698761965798923No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1150.11698761965798923No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1140.11597033600878934No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1140.11597033600878934No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1110.11291848506118961No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1080.10986663411358989No Hit
GTATCAACGCAGAGTACGGGAGGCAGCGGCCCAGTGAAATGCTTCCAACG1050.10681478316599018No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1050.10681478316599018No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1030.10478021586759036No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT990.10071108127079073No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)