FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004691652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98301
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14641.489303262428663No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4020.40894802697836236No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3710.3774122338531653No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3180.32349620044557026No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2850.2899258400219733No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2690.2736493016347748No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2180.2217678355255796No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2060.2095604317351807No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2010.20447401348918118No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1860.1892147587511826No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1830.18616290780358288No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1700.1729382203639841No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1700.1729382203639841No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1590.16174810022278513No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1570.1597135329243853No Hit
GTATCAACGCAGAGTACGGGTCTGGTGCAACGTGGGGGCTGGAGGGTGGG1520.15462711467838577No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1490.15157526373078606No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1440.14648884548478652No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1430.14547156183558663No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1410.1434369945371868No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1330.13529872534358756No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1330.13529872534358756No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1310.13326415804518774No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1240.12614317250078838No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.12207403790398877No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1190.12105675425478886No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1160.11800490330718914No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1120.11393576871038952No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTT1110.11291848506118961No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1090.1108839177627898No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1050.10681478316599018No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1040.10579749951679027No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1040.10579749951679027No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT1040.10579749951679027No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1040.10579749951679027No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1030.10478021586759036No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1030.10478021586759036No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)