FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1408999
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42100.2987936826072978No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39210.27828266734043106No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTCCA33280.2361960512392131No Hit
GTAATAGACGGCCGTGTCCGCAGCGGTCACAGACTCCAAAGTCAGGGAGA30830.218807820303634No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG29160.20695543431897395No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28590.20291000916253313No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28330.2010647275122268No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28020.19886458400609225No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG27830.1975161089539453No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26560.18850261781591043No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25080.17799870688339736No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23730.16841743677603746No Hit
GTACGTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA23020.16337839842327778No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTACTTAGCCTGGTACC22950.16288159182511838No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22890.1624557575981246No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21330.1513840676962865No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21250.15081628872696148No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21240.15074531635579586No Hit
GTTTATTACTGTCAGCAATATGGTACGTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA20950.1486871175919926No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG20160.14308030026990792No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC19830.14073821202144218No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAATATGGTACGTCA19760.14024140542328278No Hit
ATATGGTACGTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT19760.14024140542328278No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC19310.13704764872082947No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA19180.13612500789567628No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18950.13449264335886682No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18860.13385389201837616No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18850.13378291964721054No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18820.13357000253371365No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18490.13122791428524788No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC18030.12796318521162897No Hit
GTGACGTACCATATTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGT18020.12789221284046334No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC17990.12767929572696646No Hit
GTAATAAACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGTCCCACTGCCA17940.12732443387113832No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT17790.12625984830365386No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT17620.12505331799383818No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17560.1246274837668444No Hit
ATAGAAGAGGAGCCGGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGTTGCTGCTAACACTCTGA17550.12455651139567878No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC17150.12171761654905361No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTC16740.11880774933126284No Hit
GAGTGAAGTCTGTCCCAGTCCCACTGCCACTGAACCTGTCTGGGATGCCA16650.11816899799077217No Hit
CTTCTATCGTGCATCCAACAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGACTGGGACAGACTTCA16010.1136267662361719No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15610.11078787138954675No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15390.10922647922390293No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT15240.10816189365641848No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA15130.10738119757359658No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT15020.10660050149077466No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14770.10482619221163395No Hit
GTCTATTACTGTGCGAGAGGAGGGGGGTCGTTCAGCAGTGACTGGTGGGG14510.10298091056132758No Hit
GTCCCAGTCCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTT14400.10220021447850568No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG14340.10177438025151188No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14210.10085173942635871No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG2950.044.3418440
GGTATCA11000.042.363911
TACTGAT10800.040.7358770
CGTATGC3400.039.55851741
GTATCAA30600.039.2860341
ACGAGAC3550.038.99450722
ATGCCGT3600.038.5817444
ATCTCGT3300.038.34878537
AGACCGG3650.037.94771625
TATCTCG3250.037.8278936
GCCTATC3150.037.795433
CTTATAC4050.036.6865841
GACCGGA3950.035.93490626
CCGTCTT3950.035.40345847
TATACAC4050.035.014043
CGAGCCC4050.035.01031515
TATCAAC35000.034.149412
GAGCCTA3800.033.94545731
ATCAACG35200.033.859463
TCCGAGC4100.033.75994513
Produced by FastQC (version 0.11.5)