FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1262890
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACTCCTCCCCTCACTTTCGGCGGAGGCACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT101640.8048206890544702No Hit
GTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC84490.6690210548820562No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT59530.47137913832558653No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT54990.4354298474134723No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49840.39465036543166865No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC43930.347852940477793No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43770.3465860051152515No Hit
CTTTCATACAGCTCCAACAGTAAGACCTACTTAGCTTGGTACCAGCAGAA40930.3240979024301404No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGGTCTTACTGTTGGAGCTGTATGAAAGACT37700.2985216447988344No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36070.2856147407929432No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35340.27983434820134767No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34890.27627109249419984No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33990.269144581079904No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT32550.2577421628170308No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29100.2304238690622303No Hit
GTTTATTACTGTCAGCAATATTATACTCCTCCCCTCACTTTCGGCGGAGGCACCAAGGTGGAGATCAAACGAA29020.22979040138095952No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28720.22741489757619426No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA28640.22678142989492356No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28090.22242633958618727No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27720.21949655156031006No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27720.21949655156031006No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27630.21878390041888052No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26100.20666883101457767No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25770.20405577682933587No Hit
CAGTAAGACCTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTAC25740.20381822644885939No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT24810.19645416465408705No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC24620.19494967891106907No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTGCCTCCGCCGAAAGTG24560.194474578150116No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24390.19312845932741568No Hit
ATTATACTCCTCCCCTCACTTTCGGCGGAGGCACCAAGGTGGAGATCAAA23460.18576439753264337No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23410.18536848023184918No Hit
GTCCCTGACCGATTCATTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC22620.17911298687930066No Hit
GAGTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT22100.17499544695104086No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCTTCAGCTGCACGTCCAGTCAGAGTCTTTCATA21410.16953178820008077No Hit
GTATGAAAGACTCTGACTGGACGTGCAGCTGAAGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA20900.16549343173197983No Hit
GTCCCAGACCCGCTGCCAATGAATCGGTCAGGGACCCCGGATTCCCGGGT20450.16193017602483192No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATACTCCT20100.15915875491927248No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19970.1581293699372075No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCATTGGCAGCGGGTCTGGGACAGATTT19780.15662488419418952No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAA19320.15298244502688277No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCTTCAGCTGCACGTCCAGTCAGAGTCTTTCATACAGCTCCAA19090.1511612254432294No Hit
GCTCCAACAGTAAGACCTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGG18910.14973592316037027No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18860.14934000585957605No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCAATGAATCGGTCAGGGACCCCG18670.14783552011655804No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18550.14688531859465195No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18200.14411389748909248No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17720.14031309140146805No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17550.13896697257876775No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17540.1388877891186089No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17300.1369873860747967No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA17230.1364331018536848No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16560.13112781002304238No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16410.12994005812065976No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16410.12994005812065976No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCTTCAGCTGCACGTCCAGT16350.1294649573597067No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16230.12851475583780061No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16170.12803965507684756No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCAATGAATCGGTCAGGGACC15930.12613925203303533No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15910.12598088511271766No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15670.12408048206890546No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15630.12376374822827008No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15190.12027967598128102No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15120.11972539176016914No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14820.11734988795540388No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14810.11727070449524503No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGT14650.11600376913270356No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14590.1155286683717505No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14540.11513275107095629No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14510.11489520069047976No Hit
GTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA14400.11402418262873251No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14380.11386581570841482No Hit
GACCTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGC14270.11299479764666756No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14220.11259888034587334No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14060.11133194498333188No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14020.11101521114269652No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13940.11038174346142578No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT13840.10958990885983737No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG13720.10863970733793127No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATACTCCTCCCCTCACTTT13660.10816460657697821No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG13620.10784787273634283No Hit
GTCTCTGGGCGAGAGGGCCACCTTCAGCTGCACGTCCAGTCAGAGTCTTTCATACAGCTCCAACAGTAAGACCTA13500.10689767121443673No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13340.10563073585189527No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATACTCCTCCCC13250.10491808471046568No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGGTCTTACTGTTGGAGCTG13180.10436380048935377No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13120.10388869972840073No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT12940.10246339744554157No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTGC12910.10222584706506505No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG12890.10206748014474736No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12820.10151319592363547No Hit
ACCTTCAGCTGCACGTCCAGTCAGAGTCTTTCATACAGCTCCAACAGTAA12780.10119646208300011No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16850.047.093181
GTGGTAT3750.046.1191181
TATCTCG306.8262825E-445.27405524
TGGTATC4000.043.2263952
GCTTATA702.5484042E-838.7555581
GTATCAA43350.038.3309021
ACGAGGT901.2175871E-437.0792670
TATCAAC51000.032.5070422
ATCAACG52400.031.629763
TCAACGC52550.031.4762254
CAACGCA53750.030.7734995
AACGCAG54500.030.41226
GGTCTGG27500.030.33757470
ATACGTA1800.030.12649311
CGCAGAG56650.029.1981588
GACAGGT2000.028.8244481
ACGCAGA57500.028.7676727
CGAACTG31600.028.56982853
CGGGCTA953.7200516E-728.5612617
AATACGT1900.028.5420210
Produced by FastQC (version 0.11.5)