FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1288181
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACGAGGGTGCCAGTGCCCCAGGTCTGACAGTAATAGTCAG49310.3827878225187299No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG44060.34203268019012856No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC41720.32386753103795196No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC41250.3202189754390105No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACGAG37190.28870166537155884No Hit
CCTCTGAGGAGCTTCAAGCCAACACGGCCACACTGGTGTGTCTCATAAGT36570.2838886771346573No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA34970.27146806232975024No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG33400.25928033405243517No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACACGGCCACACTGGTGT33290.2584264167845978No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT33280.25834878794206717No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC31630.24554002892450671No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT31350.243366421333648No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGACTGGAGATGGTGAGGTAGC30930.24010600994735987No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGGT30550.23715611393119446No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG30400.2359916812932344No Hit
GACTATTACTGTCAGACCTGGGGCACTGGCACCCTCGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAG30350.23560353708058107No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29720.2307129200011489No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT29680.23040240463102624No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC28510.2213198300549379No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACACGG28420.22062117047216193No Hit
CACCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGG28250.21930148014914055No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG27650.21464374959730037No Hit
CCTGCACTCTGAGCAGTGGGCACACCAACTACGCCATCGCATGGCATCAG26090.20253365016251598No Hit
CACCTGCACTCTGAGCAGTGGGCACACCAACTACGCCATCGCATGGCATC25640.1990403522486359No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25540.19826406382332917No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC25460.19764303308308384No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG25300.19640097160259312No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC24950.1936839621140197No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT23720.18413561448274737No Hit
GGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC23520.182583037632134No Hit
CTATTACTGTCAGACCTGGGGCACTGGCACCCTCGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCC23130.1795555127734379No Hit
CACCAGTGTGGCCGTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22780.17683850328486447No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC22730.17645035907221113No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC22430.17412149379629105No Hit
GCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG22080.17140448430771763No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT21440.1664362383857548No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC21180.1644178884799574No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGACTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGC21010.16309819815693602No Hit
CTTATGAGACACACCAGTGTGGCCGTGTTGGCTTGAAGCTCCTCAGAGGA20630.16014830214077058No Hit
GCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGTCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGAC20510.15921675603040256No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT20390.15828520992003453No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20320.15774180802231985No Hit
CTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT20090.15595634464411445No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC19850.15409325242337837No Hit
GTAATATACGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGTAGATACA19600.15215253136011164No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19550.15176438714745832No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACACGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19340.15013418145431426No Hit
TCCTCAGACTGGAGACTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATC19280.14966840839913026No Hit
GTTCTTGATGAACCTTAACCATGATGGCAGCCACACCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAG19120.14842634691863954No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18940.1470290277530875No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT18760.14563170858753546No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGG18580.1442343894219834No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT18460.14330284331161536No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT17360.13476367063324177No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT17320.13445315526311907No Hit
GTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCACTGGCACCCTCGTGTTCGGCGGAGGGACCAAG16940.13150325924695366No Hit
GTGTGGCCGTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA16500.12808759017560423No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16490.12800996133307355No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16400.1273113017502975No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT16120.12513769415943876No Hit
CCCTTGGTGTGGCTGCCATCATGGTTAAGGTTCATCAAGAACCGAGGGCC16010.1242837768916014No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTC15920.12358511730882539No Hit
CGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC15770.12242068467086535No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTC15410.11962604633976126No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG14850.11527883115804377No Hit
CTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGC14830.11512357347298245No Hit
CTCCAGCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGTCTCCAGTCTG14800.11489068694539044No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG14690.11403676967755307No Hit
GCTTGGTCCCTCCGCCGAACACGAGGGTGCCAGTGCCCCAGGTCTGACAGTAATAGTCAGCCTCATCCTCAGA14680.1139591408350224No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA14670.11388151199249175No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCAC14620.11349336777983839No Hit
CTACCTCACCATCTCCAGTCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCACTGGCACCCT14390.111707904401633No Hit
ACCTTAACCATGATGGCAGCCACACCAAGGGGGACGGGATCCCTGATCGC14360.11147501787404099No Hit
GTATCAACGCAGAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTC14330.11124213134644897No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC14290.11093161597632631No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG14280.11085398713379564No Hit
TCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCT14180.11007769870848895No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT14170.11000006986595827No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG14150.10984481218089692No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCGTGTTGGCTTGAAG14060.10914615259812091No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA13980.10852512185787555No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC13960.10836986417281423No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG13930.10813697764522222No Hit
TGGTTAAGGTTCATCAAGAACCGAGGGCCCTTCTCTGGCTGCTGCTGATGCCATGCGATGGCGTAGTTGGTGTGC13550.10518708162905678No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCA13480.1046436797313421No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13350.10363450477844341No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC13320.1034016182508514No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC13320.1034016182508514No Hit
GTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCA13160.10215955677036069No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACACGGCC13160.10215955677036069No Hit
CCTTAACCATGATGGCAGCCACACCAAGGGGGACGGGATCCCTGATCGCT13120.10184904140023801No Hit
GAGTGCAGGTGAGCTTGACCGAGGCTCCCAGGGAGGCAGAGGCAGAGGGC13110.10177141255770733No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)