FastQCFastQC Report
Wed 8 May 2024
EGAF00004691658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1288181
Total Bases95 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG48000.37261844414721224No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACGAGGGTGCCAGTG46580.3615951485078572No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC45070.3498731932857262No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC43410.336986805425635No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG42910.3331053632991016No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCC42180.32743845779436276No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACACGGCCACACTGGTGT40310.3129218642411276No Hit
GGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTC38720.3005788782787512No Hit
CCTCTGAGGAGCTTCAAGCCAACACGGCCACACTGGTGTGTCTCATAAGT37980.29483434393148167No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA35290.2739521852907317No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACGAG34340.26657744525031807No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT34160.26518012608476604No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA33510.26013425132027257No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA33290.2584264167845978No Hit
GACTATTACTGTCAGACCTGGGGCACTGGCACCCTCGTGTTCGGCGGAGG31740.2463939461923441No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA30330.23544827939551974No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGACTGGAGATGGTGAGGTAGC29510.22908271430800486No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC29240.22698673555967677No Hit
CTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAG28920.22450261259869536No Hit
CACCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGG28720.22295003574808198No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGC28330.21992251088938589No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27430.21293591506162562No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC27280.2117714824236656No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT27200.21115045168342028No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA27140.21068467862823625No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT26540.20602694807639607No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC26230.20362045395794534No Hit
CCTGCACTCTGAGCAGTGGGCACACCAACTACGCCATCGCATGGCATCAG26010.20191261942227065No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGG25960.2015244752096173No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT25630.1989627234061052No Hit
CTATTACTGTCAGACCTGGGGCACTGGCACCCTCGTGTTCGGCGGAGGGA25440.19748777539802248No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT25360.19686674465777712No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGGT25120.19500365243704107No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT24740.19205375642087566No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTG24330.1888709738771182No Hit
GCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCT24100.1870855104989128No Hit
CACCTGCACTCTGAGCAGTGGGCACACCAACTACGCCATCGCATGGCATC23880.18537767596323806No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC22730.17645035907221113No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACACGGCCACACTG21860.1696966497720429No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT21630.1679111863938375No Hit
GCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGTCTCCAGTCTGAGGAT21570.1674454133386535No Hit
CACCAGTGTGGCCGTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACA20870.16201139436150666No Hit
GTTCTTGATGAACCTTAACCATGATGGCAGCCACACCAAGGGGGACGGGA20660.1603811886683626No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGA20660.1603811886683626No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20470.15890624066027986No Hit
CTTATGAGACACACCAGTGTGGCCGTGTTGGCTTGAAGCTCCTCAGAGGA20350.15797469454991186No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGACTGGAGATGGTGAGGTA20260.15727603496713582No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT20200.1568102619119518No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC20130.15626686001423712No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT19930.15471428316362373No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19630.15238541788770368No Hit
GTAATATACGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGTAGATACA19320.14997892376925293No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG19270.14959077955659958No Hit
GTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCACTGGCACCC19120.14842634691863954No Hit
TCCTCAGACTGGAGACTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCT18710.1452435643748821No Hit
CTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCT18670.14493304900475942No Hit
GTATCAACGCAGAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTG18590.14431201826451406No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT18460.14330284331161536No Hit
GTGTGGCCGTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTG18340.14237129720124736No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17930.1391885146574899No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC17570.1363938763263858No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT17510.1359281032712018No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTC17140.13305583609756702No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16790.1303388266089936No Hit
CTCCAGCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGTCTCCAGTCTG16700.12964016702621758No Hit
ACCTTAACCATGATGGCAGCCACACCAAGGGGGACGGGATCCCTGATCGC16620.12901913628597222No Hit
CCCTTGGTGTGGCTGCCATCATGGTTAAGGTTCATCAAGAACCGAGGGCC16610.12894150744344157No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT16500.12808759017560423No Hit
TCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTG16280.12637975563992948No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA16230.12599161142727613No Hit
CGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACA16090.12490480763184676No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCC16010.1242837768916014No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT15990.12412851920654007No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTC15970.12397326152147875No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG15910.12350748846629471No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA15860.12311934425364138No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT15390.11947078865469991No Hit
GTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCA15360.11923790212710793No Hit
GAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCC15280.11861687138686257No Hit
GTGGGCACACCAACTACGCCATCGCATGGCATCAGCAGCAGCCAGAGAAG15170.11776295411902521No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA15120.11737480990637185No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGC15090.11714192337877985No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTC14870.11543408884310512No Hit
CTACCTCACCATCTCCAGTCTCCAGTCTGAGGATGAGGCTGACTATTACT14610.11341573893730772No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTG14600.11333811009477705No Hit
GCTTGGTCCCTCCGCCGAACACGAGGGTGCCAGTGCCCCAGGTCTGACAG14580.11318285240971572No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAA14510.11263945051200103No Hit
CTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCG14420.111940790929225No Hit
CTCTGAGCAGTGGGCACACCAACTACGCCATCGCATGGCATCAGCAGCAG14270.11077635829126496No Hit
GTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCC14250.11062110060620363No Hit
GGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGG14190.11015532755101962No Hit
GTATATTACTGTGCGAAACCCCCGCAGATGGCTACCACCCCCTCCTGGGG14170.11000006986595827No Hit
CCTTAACCATGATGGCAGCCACACCAAGGGGGACGGGATCCCTGATCGCT13760.10681728732220085No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC13730.10658440079460883No Hit
CCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCTGAGCAGTGGGCACACC13610.10565285468424081No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT13600.10557522584171014No Hit
CCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCT13500.10479893741640345No Hit
GGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCATCCCGGCAG13460.10448842204628075No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACACGGCC13430.10425553551868877No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC13420.10417790667615809No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGT13410.10410027783362742No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG13210.10254770098301402No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTT13200.10247007214048336No Hit
CTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAA13130.10192667024276868No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCGTGTTGGCTTGAAG13120.10184904140023801No Hit
GTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAA13100.10169378371517666No Hit
TGGTTAAGGTTCATCAAGAACCGAGGGCCCTTCTCTGGCTGCTGCTGATG13010.10099512413240064No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA12990.10083986644733932No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC12960.1006069799197473No Hit
GGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACT12930.10037409339215529No Hit
AACTACGCCATCGCATGGCATCAGCAGCAGCCAGAGAAGGGCCCTCGGTT12920.10029646454962461No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)