FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1589370
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAAAA142850.898783794836948No Hit
ATATTATACTACTCCTAAGACGTTCGGCCGAGGGACCAAGGTGGAAATCCAACGAACTGTGGCTGCACCAT139120.8753153765328401No Hit
GTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC107890.6788224264960331No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT98470.6195536596261412No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC86980.5472608643676425No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA76500.48132278827459934No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC62920.39588012860441557No Hit
ACTTAGGAGGCTGTCCTGATTTTTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAACACT62430.39279714603899657No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC62300.39197921188898743No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG61170.384869476585062No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGGATCCGGGGTCCCT60610.3813460679388688No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT56240.3538508968962545No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC54820.3449165392576933No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG54400.34227398277304844No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA48390.3044602578380113No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG47520.2989863908341041No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT46550.29288334371480523No Hit
GTTTATTACTGTCAGCAATATTATACTACTCCTAAGACGTTCGGCCGAGGGACCAAGGTGGAAATCCAACGAA45760.28791282080321134No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG44830.28206144573006914No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG44110.27753134889924935No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC42180.26538817267219084No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT42140.2651365006260342No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA42040.2645073205106426No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT41590.2616760099913802No Hit
GTCCCTGACCGATTCAGCGGCAGCGGGTCTGGGACAGATTTCACTCTCAC41410.26054348578367525No Hit
GTGCAGCCACAGTTCGTTGGATTTCCACCTTGGTCCCTCGGCCGAACGTC41290.25978846964520536No Hit
CTCCTAAGACGTTCGGCCGAGGGACCAAGGTGGAAATCCAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC40660.25582463491823804No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT40380.25406293059514146No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCG39290.24720486733737268No Hit
GTGCTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC35680.22449146517173468No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAA35540.22361061301018645No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG34160.21492792741778188No Hit
TCTTAGGAGTAGTATAATATTGCTGACAGTAATAAACTGCCACATCTTCA32660.20549022568690736No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32590.20504979960613323No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA32300.2032251772714975No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC32270.20303642323688No Hit
GTCAGGGACCCCGGATCCCCGGGTAGATGCCCAGTAAATGAGCAACTTAGGAGGCTGTCCTGATTTTTGCTGGTA32190.20253307914456672No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATACTACT31450.19787714629066863No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG31250.19661878605988536No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA29590.18617439614438427No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT29580.1861114781328451No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA29570.1860485601213059No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG29150.18340600363666107No Hit
AGATGGTGCTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA28180.17730295651736222No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAACAATAAGAACTA27840.1751637441250307No Hit
GGCAGGGGCAGCAAGATGGTGCTGCAGACCCAGGTCTTCATTTCTCTGTT27810.17497499009041317No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC27350.17208076155961166No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT27140.17075948331728924No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA26280.1653485343249212No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT26110.1642789281287554No Hit
CAGCAAGATGGTGCTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGA25310.15924548720562234No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT25050.15760961890560413No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG24850.15635125867482083No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA24700.1554074885017334No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATACTACTCCTAAGACGTT23880.150248211555522No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT23880.150248211555522No Hit
CTACTTAGCTTGGTACCAGCAAAAATCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGGATC23720.14924152337089538No Hit
ATTCAGCGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC23620.14861234325550376No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG23400.14722814700164216No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT23180.14584395074778056No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG23120.1454664426785456No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC23060.1450889346093106No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC23000.14471142654007563No Hit
AATCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGGA22910.14414516443622316No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG22820.1435789023323707No Hit
ACTTAGCTTGGTACCAGCAAAAATCAGGACAGCCTCCTAAGTTGCTCATT22650.14250929613620492No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA22470.14137677192849996No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTGGATTTCCACCTTGGTCC22310.14037008374387336No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA22270.14011841169771672No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA22090.13898588749001178No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC22040.13867129743231596No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC21890.1377275272592285No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC21890.1377275272592285No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGCTGCAGACCCAGGTCTTCA21650.13621749498228858No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC21360.13439287264765284No Hit
ATACTACTCCTAAGACGTTCGGCCGAGGGACCAAGGTGGAAATCCAACGA21320.1341412006014962No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC21290.1339524465668787No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCG21280.13388952855533953No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG21180.1332603484399479No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG21170.13319743042840873No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC20410.12841566155143233No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCGCTGAATCGGTCAGGGACC20290.12766064541296238No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC20270.12753480938988404No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT20210.12715730132064906No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA20180.12696854728603157No Hit
GTAAATGAGCAACTTAGGAGGCTGTCCTGATTTTTGCTGGTACCAAGCTA20160.12684271126295324No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATACTACTCCTA19940.12545851500909166No Hit
CAATAAGAACTACTTAGCTTGGTACCAGCAAAAATCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTAC19820.12470349887062168No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT19770.12438890881292589No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGCTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT19750.12426307278984754No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA19650.12363389267445592No Hit
GAACTACTTAGCTTGGTACCAGCAAAAATCAGGACAGCCTCCTAAGTTGC19630.1235080566513776No Hit
GCAAGATGGTGCTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC19610.12338222062829927No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC19340.12168343431674185No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC19210.12086550016673274No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC19180.12067674613211524No Hit
ACCCGGGGATCCGGGGTCCCTGACCGATTCAGCGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC19160.12055091010903692No Hit
CCTAAGACGTTCGGCCGAGGGACCAAGGTGGAAATCCAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG18770.11809710765900955No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG18660.11740500953207876No Hit
GCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGGATCCGGGGTCCCTGACCGATTCAGCGGCAGCGGGT18380.11564330520898217No Hit
GCTTGGTACCAGCAAAAATCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGGATCCGGGGTC18030.11344117480511147No Hit
TTATTACTGTCAGCAATATTATACTACTCCTAAGACGTTCGGCCGAGGGA17750.11167947048201488No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG17220.10834481587043923No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17180.10809314382428258No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA17000.10696061961657764No Hit
ACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAACAATAA16500.10381471903961947No Hit
CTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAACAATAAGAACTACT16490.10375180102808029No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16490.10375180102808029No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT16470.10362596500500197No Hit
CATTTACTGGGCATCTACCCGGGGATCCGGGGTCCCTGACCGATTCAGCGGCAGCGGGTCTGGGACAGATTTCA16410.103248456935767No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTGGATTTCCACCTTGGTCCCTCGGCCGAACGTCTTAGGAGTAGT16250.10224176875114038No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC16080.1011721625549746No Hit
GAGTAGTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT16040.10092049050881796No Hit
GCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAAAAATCAGGACAGCCTCCTAAGTTGCTCATTTACTGGG16030.10085757249727881No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)