FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences707880
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTACTTCACCAGCGACTTTTGGCCAGGGGA29300.4139119624795163No Hit
GTATGGTACTTCACCAGCGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT26630.3761937051477651No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA25200.3559925411086625No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25110.3547211391761315No Hit
GTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC23940.3381929140532294No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22880.32321862462564277No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21210.29962705543312423No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGCCA21070.2976493190936317No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19710.27843702322427527No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19500.2754704187150364No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGCGGCCCTGCT16690.23577442504379276No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16370.2312538848392383No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15970.22560320958354524No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT15720.22207153754873707No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15700.22178900378595243No Hit
GTGTATTACTGTCAGCAGTATGGTACTTCACCAGCGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA15640.22094140249759847No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA15320.21642086229304403No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14750.2083686500536814No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCTGGGCCAGTCAGAGTGTTTGGGG14560.20568457930722722No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC14280.20172910662824206No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14070.1987625021190032No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13890.19621969825394134No Hit
CTCCCAGACTCCTCATCTATGACGCATCCAGCAGGGCCGCTGGCATCCCA13880.19607843137254902No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13760.1943832287958411No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13630.19254675933774085No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13210.18661355031926316No Hit
GTACTTCACCAGCGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA12900.18223427699610104No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT12830.18124540882635476No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12810.1809628750635701No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTC12680.17912640560546986No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12660.1788438718426852No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12160.1717805277730689No Hit
GTCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTGCCC11340.16019664349889812No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11260.15906650844775952No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC11230.15864270780358253No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11170.15779510651522857No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10760.15200316437814318No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA10470.14790642481776572No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGC10430.1473413572921964No Hit
CTGCTACTCTGGCTCCCAGATGCCACCGGAGAAATTGTGTTGACGCAGTC10020.14154941515511105No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9790.13830027688308752No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT9730.1374526755947336No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9650.13632254054359497No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTACTTCA9360.1322258009832175No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCTGGGCCAGTCAGA9330.13180200033904052No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9130.128976662711194No Hit
GTGAAGTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGT9040.12770526077866307No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8920.12601005820195513No Hit
ACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGACTCCTCATC8760.12374978809967792No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8690.12276091992993164No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8610.12163078487879302No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8580.12120698423461604No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8470.11965304853930045No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8460.11951178165790811No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGCCACCGGAGAAATTGTGTTGACGCAGTCTC8450.1193705147765158No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8440.11922924789512347No Hit
GCAGAAACCTGGCCAGGCTCCCAGACTCCTCATCTATGACGCATCCAGCAGGGCCGCTGGCATCCCAGACAGGTT8360.11809911284398486No Hit
CCTGGAGACTGCGTCAACACAATTTCTCCGGTGGCATCTGGGAGCCAGAGTAGCAGGAGGAAGAGAAGCTGCG8330.11767531219980787No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8280.11696897779284625No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8270.11682771091145391No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8240.11640391026727694No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8220.1161213765044923No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC8120.11470870769056901No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8100.11442617392778437No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8060.11386110640221507No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8030.11343730575803809No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8010.11315477199525344No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7970.11258970446968414No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA7870.11117703565576087No Hit
TCTATGACGCATCCAGCAGGGCCGCTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT7750.10948183307905296No Hit
CTCCTCATCTATGACGCATCCAGCAGGGCCGCTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGA7700.10877549867209131No Hit
GCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGACTCCTCATCTATGACGCATCCAGCAGGGCCGCTGGCATC7630.10778663050234503No Hit
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCT7570.10693902921399107No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT7550.10665649545120641No Hit
CTCTTCCTCCTGCTACTCTGGCTCCCAGATGCCACCGGAGAAATTGTGTT7540.10651522856981409No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7520.10623269480702943No Hit
GTCTGGGATGCCAGCGGCCCTGCTGGATGCGTCATAGATGAGGAGTCTGGGAGCCTGGCCAGGTTTCTGCTGGTA7520.10623269480702943No Hit
GTCAGAGTGTTTGGGGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGC7500.10595016104424479No Hit
GGTTTCTGCTGGTACCAGGCTAAGTAGCTGCCCCAAACACTCTGACTGGCCCAGCAGGAGAGGGTGGCTCTTTCC7400.10453749223032154No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA7360.10397242470475221No Hit
ATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGCCACCGGAGAAATTGTGTTGACG7360.10397242470475221No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7300.10312482341639825No Hit
ATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCAC7260.10255975589082895No Hit
GCCCTGCTGGATGCGTCATAGATGAGGAGTCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTG7140.10086455331412103No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA7140.10086455331412103No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT100.008013045139.1757270
AATATCG200.00784842350.91185436
GAGTACA200.00868750249.6202620
TATAGGG200.0086996449.6027342
TCGTATG2500.048.29154240
GCCGTCT2750.046.0377446
CGTATGC2700.044.90963741
CCGTCTT2850.044.596647
CTAATCT2200.044.49873734
GCTAATC2400.042.09992633
TCCGAGC3000.041.8867513
ACGAGAC2850.041.80024722
CCCACGA2900.041.05343619
ATACACA3000.040.7844664
CGAGACT2950.040.3975723
GTATGCC3200.040.1886642
ATGCCGT3150.039.98142244
AATCTCG2500.039.37183436
AAAGGGG3100.038.9882868
CGTCTTC3300.038.68739748
Produced by FastQC (version 0.11.5)