FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences707880
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTACTTCACCAGCGACTTTTGGCCAGGGGA28310.399926541221676No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27260.3850935186754817No Hit
GTATGGTACTTCACCAGCGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT25410.3589591456179013No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25330.35782901056676275No Hit
GTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC22200.3136124766909646No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA22190.31347120980957227No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGCCA20680.292139910719331No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19940.2816861614962988No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19850.2804147595637679No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18470.2609199299316268No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18200.25710572413403404No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT17270.24396790416454767No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17060.24100129965530878No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16830.2377521613832853No Hit
GTGTATTACTGTCAGCAGTATGGTACTTCACCAGCGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA16370.2312538848392383No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGCGGCCCTGCT15510.2191049330394982No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15390.2174097304627903No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCTGGGCCAGTCAGAGTGTTTGGGG14610.20639091371418886No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14470.20441317737469625No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC13950.1970672995422953No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13910.196502232016726No Hit
CTCCCAGACTCCTCATCTATGACGCATCCAGCAGGGCCGCTGGCATCCCA13630.19254675933774085No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13600.19212295869356388No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA13480.19042775611685595No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13300.1878849522517941No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13180.18618974967508617No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12880.18195174323331637No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTC12790.18068034130078545No Hit
GTCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTGCCC12420.17545346668926937No Hit
GTACTTCACCAGCGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA12110.17107419336610724No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12090.1707916596033226No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11420.16132677855003671No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC11210.15836017404079786No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11130.15723003898965926No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11010.15553483641295135No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10530.14875402610611968No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGC10090.14253828332485732No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10090.14253828332485732No Hit
CTGCTACTCTGGCTCCCAGATGCCACCGGAGAAATTGTGTTGACGCAGTC10080.142397016443465No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA9930.1402780132225801No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTACTTCA9880.13957167881561847No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9660.1364638074249873No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9610.13575747301802565No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCTGGGCCAGTCAGA9550.1349098717296717No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9510.13434480420410239No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT9350.13208453410182516No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9220.1302480646437249No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9050.1278465276600554No Hit
ACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGACTCCTCATC9040.12770526077866307No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGCCACCGGAGAAATTGTGTTGACGCAGTCTC9040.12770526077866307No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8820.12459738938803187No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8680.12261965304853929No Hit
GTGAAGTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGT8590.12134825111600836No Hit
CTCCTCATCTATGACGCATCCAGCAGGGCCGCTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGA8560.12092445047183137No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC8460.11951178165790811No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8440.11922924789512347No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8430.11908798101373114No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8410.1188054472509465No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8380.11838164660676952No Hit
GCAGAAACCTGGCCAGGCTCCCAGACTCCTCATCTATGACGCATCCAGCAGGGCCGCTGGCATCCCAGACAGGTT8330.11767531219980787No Hit
CCTGGAGACTGCGTCAACACAATTTCTCCGGTGGCATCTGGGAGCCAGAGTAGCAGGAGGAAGAGAAGCTGCG8320.11753404531841555No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8180.11555630897892297No Hit
GCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGACTCCTCATCTATGACGCATCCAGCAGGGCCGCTGGCATC8070.11400237328360739No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8060.11386110640221507No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7760.10962309996044527No Hit
GGTTTCTGCTGGTACCAGGCTAAGTAGCTGCCCCAAACACTCTGACTGGCCCAGCAGGAGAGGGTGGCTCTTTCC7620.10764536362095271No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7580.10708029609538339No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7560.10679776233259874No Hit
GATGAGGAGTCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTGCCCCAAACACTCTGACTGGC7450.10524382663728316No Hit
CTCTTCCTCCTGCTACTCTGGCTCCCAGATGCCACCGGAGAAATTGTGTT7250.10241848900943663No Hit
GTCTGGGATGCCAGCGGCCCTGCTGGATGCGTCATAGATGAGGAGTCTGGGAGCCTGGCCAGGTTTCTGCTGGTA7170.10128835395829801No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7130.10072328643272871No Hit
GCCCTGCTGGATGCGTCATAGATGAGGAGTCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTG7080.10001695202576709No Hit
TCTATGACGCATCCAGCAGGGCCGCTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT7080.10001695202576709No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACTA150.002390911668.7381239
AACGTCC150.002795716566.079215
TGAGACG200.00627654153.871951
GGCGTAA253.1809884E-452.8521512
GCGTAAA253.1809884E-452.8521513
TATGGTT804.359371E-544.12998670
CGTAAAG307.819058E-444.0403414
GAGCGTA406.4129825E-541.34335722
AGCGTAG505.109803E-639.71492423
TACCTAT450.001821612137.08039568
GTATCAA30300.033.817241
GGTATCA13550.033.6634751
TATCAAC34000.030.0186772
CTCTATT450.005717757629.3602282
CGTACCC450.005723687429.35400210
AACGCAG35000.029.2512346
ATCAACG35300.028.8175643
CAACGCA35600.028.665475
TCAACGC35600.028.665474
AGAGTAC35550.028.61288611
Produced by FastQC (version 0.11.5)