FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences781648
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42840.5480727897979654No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31580.4040181769799194No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26900.34414467893476347No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25250.3230354328290995No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24510.3135682557877715No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24500.31344032096288865No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22090.28260802816613106No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA21910.28030520131824044No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20220.25868421591304525No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20210.2585562810881624No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19970.25548584529097496No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19710.25215953984402184No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC19240.2461466030745297No Hit
GTTTAGGGGCTGTCCCCGGTTTCTGCTGATACCAGGCCAACCAGTTATTA19130.2447393200008188No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTGGGTCCCTTGGCCGAACGTC18570.2375749698073813No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18540.23719116533273288No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17610.22529322661863138No Hit
CTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGG17270.2209434425726158No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTGAAAGTGTTAATAACTGGTTGGCCTGGTATCAGCAGAAA16860.2156981147524205No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTGGGTCC16580.21211593965570183No Hit
CTATAAGAACTTTTCTCCGTGGACGTTCGGCCAAGGGACCCAGGTGGAAATCAAACGAACTGTGGCTGCACCAT16580.21211593965570183No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16370.20942930833316278No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16280.20827789490921747No Hit
GAGTGAATTCTGTCCCAAATCCAGAGCCGCTGAACCTTGACGGGACCCCA16080.20571919841156122No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16030.20507952428714715No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTGAAA15810.20226495813972528No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15760.20162528401531124No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15690.20072974024113155No Hit
ACTTATTACTGCCAACACTATAAGAACTTTTCTCCGTGGACGTTCGGCCA15640.2000900661167175No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14410.18435408265613165No Hit
GCCCCTAAACTCCTGATCTATCGGGCGTCTAGTTTAGAAAGTGGGGTCCC14120.18064397273453012No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13220.16912983849507707No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13220.16912983849507707No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13080.1673387509467177No Hit
GTTAATAACTGGTTGGCCTGGTATCAGCAGAAACCGGGGACAGCCCCTAA13050.16695494647206927No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13010.16644320717253802No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12960.16580353304812395No Hit
CTCCTGATCTATCGGGCGTCTAGTTTAGAAAGTGGGGTCCCGTCAAGGTT12810.16388451067488177No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12540.16043027040304586No Hit
GTTCTTATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATT12430.15902298732933495No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG12370.15825537838003806No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTGACAT11610.1485323316889444No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAAATCCAGAGCCG11510.14725298344011628No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11480.14686917896546783No Hit
GTTATTAACACTTTCACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGGGA11200.1432870038687491No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11150.14264732974433506No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11100.14200765561992099No Hit
GGGTGGAAGGGGACTGGGTCATCTGGATGTCACACTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG10980.14047243772132725No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAAC10920.1397048287720304No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG10890.13932102429738194No Hit
GTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGG10880.13919308947249914No Hit
GATCAGGAGTTTAGGGGCTGTCCCCGGTTTCTGCTGATACCAGGCCAACC10850.1388092849978507No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10850.1388092849978507No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC10700.13689026262460852No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC10690.1367623277997257No Hit
GTCCCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTGAAAGTGTTAATAA10620.13586678402554603No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10510.1344595009518351No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTGACATCCAGATGACCCAGTCCCCTTCCACC10500.1343315661269523No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10420.13330808752788978No Hit
GCTCTGGATTTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCT10420.13330808752788978No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10290.13164493480441325No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10260.1312611303297648No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10250.131133195504882No Hit
ATCCAGAGCCGCTGAACCTTGACGGGACCCCACTTTCTAAACTAGACGCCCGATAGATCAGGAGTTTAGGGG10170.1301097169058195No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10140.12972591243117107No Hit
GTGTGACATCCAGATGACCCAGTCCCCTTCCACCCTGTCTGCATCTGTAG10120.12947004278140545No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9920.12691134628374923No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9910.1267834114588664No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9890.12652754180910078No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9880.12639960698421795No Hit
CTGCCAACACTATAAGAACTTTTCTCCGTGGACGTTCGGCCAAGGGACCCAGGTGGAAATCAAACGAACTGTGG9870.12627167215933516No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9780.12512025873538984No Hit
GGTATCAGCAGAAACCGGGGACAGCCCCTAAACTCCTGATCTATCGGGCGTCTAGTTTAGAAAGTGGGGTCCCGT9650.12345710601191329No Hit
CACTATAAGAACTTTTCTCCGTGGACGTTCGGCCAAGGGACCCAGGTGGA9610.12294536671238206No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9530.12192188811331955No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9500.12153808363867112No Hit
GCCAAGGGACCCAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC9370.11987493091519456No Hit
AGTTTAGAAAGTGGGGTCCCGTCAAGGTTCAGCGGCTCTGGATTTGGGACAGAATTCACTCTCACCATCAGCAGC9230.1180838433668352No Hit
GTGAAAGTGTTAATAACTGGTTGGCCTGGTATCAGCAGAAACCGGGGACAGCCCCTAAACTCCTGATCTATCGGG9210.11782797371706957No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9210.11782797371706957No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9180.11744416924242113No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9020.11539721204429615No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8990.11501340756964772No Hit
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCT8940.11437373344523366No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT8910.11398992897058521No Hit
GCCACAGTTCGTTTGATTTCCACCTGGGTCCCTTGGCCGAACGTCCACGG8760.11207090659734303No Hit
CCCCTAAACTCCTGATCTATCGGGCGTCTAGTTTAGAAAGTGGGGTCCCG8690.11117536282316337No Hit
ACCTTGACGGGACCCCACTTTCTAAACTAGACGCCCGATAGATCAGGAGTTTAGGGGCTGTCCCCGGTTTCTG8670.11091949317339775No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8470.10836079667574151No Hit
GCTGATGGTGAGAGTGAATTCTGTCCCAAATCCAGAGCCGCTGAACCTTGACGGGACCCCACTTTCTAAACTAGA8470.10836079667574151No Hit
CTTATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC8460.1082328618508587No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8420.10772112255132746No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC8360.10695351360203058No Hit
GTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA8310.10631383947761652No Hit
CACCATCACTTGCCGGGCCAGTGAAAGTGTTAATAACTGGTTGGCCTGGTATCAGCAGAAACCGGGGACAGCCC8120.1038830778048431No Hit
GTCTCCTACAGATGCAGACAGGGTGGAAGGGGACTGGGTCATCTGGATGTCACACTTGGCACCTGGGAGCCAGAG8100.10362720815507748No Hit
GCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACACTATAAGAAC8020.10260372955601497No Hit
ACCGGGGACAGCCCCTAAACTCCTGATCTATCGGGCGTCTAGTTTAGAAAGTGGGGTCCCGTCAAGGTTCAGCGG7980.10209199025648374No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC7940.10158025095695249No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTATC200.00864768749.6784519
ACCTTAC200.00866741549.64981515
TACGTAT606.5069715E-445.70810370
CGGGTAC608.065399E-944.1473117
GACCGGA805.1868148E-542.85134570
GTATCAA32900.035.6013871
ATAGCTG450.00275427334.0849465
CGTTGCC400.003166364533.1295822
GGTACCT502.3655247E-433.1189719
CTAATAT601.789848E-533.0786933
ATCAACG37250.030.991853
AACGCAG37450.030.92066
AATGGTA3350.030.69946970
TCAACGC37650.030.6606254
CGCAGAG37050.030.6293418
TTGAACG753.091467E-530.53598464
ATCGGGA600.004736011430.51254569
GTACGTA600.004736011430.51254569
AGAGTAC37400.030.43116411
CCTACGT952.2250242E-730.2844751
Produced by FastQC (version 0.11.5)