FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004691675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1986100
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT87360.4398570061930417No Hit
GTAATATATGGCCGTGTCGTCGGCTCTCAGGCTGTTCAGTTGCAGAAACA72760.36634610543275764No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC71800.36151251195810885No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCT70480.3548663209304667No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGAGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG56590.2849302653441418No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC54830.2760686773072856No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG54230.2730476813856301No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC53580.26977493580383666No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGACT53500.2693721363476159No Hit
ATATATTACTGTGCGAGAGATGGCCCAGGGGGATCTAGTAGAGATGTTTA47780.24057197522783344No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCTATGGCCCTGTT47690.2401188258395851No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT46290.23306983535572226No Hit
GTTTATTACTGTCAGCAGCGTAGCAACTGGCCTCCGTTCACTTTCGGCCC45340.22828659181310107No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTTCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT44740.22526559589144554No Hit
GCCTAGAGCCTGAAGACTTTGCAGTTTATTACTGTCAGCAGCGTAGCAAC44300.2230501988822315No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA43080.2169075071748653No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG42330.21313126227279594No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC42000.21146971451588542No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT40380.20331302552741554No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGAACGGAGGCCAGTTGCTACGCTG40340.20311162579930517No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG39670.19973818035345653No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG39500.19888223150898746No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG38990.19631438497558026No Hit
GTAATAAACTGCAAAGTCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA38710.1949045868788077No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG38100.1918332410251246No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT37030.18644579829817232No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT35600.17924575801822668No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGACTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGG35420.17833945924173003No Hit
GTCTTTGTCTCCAGGAGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTTCTTAGCCTGGTA34820.17531846332007453No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC34700.1747142641357434No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC34480.17360656563113638No Hit
GTACTACGGTATGGACATCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGT34420.17330446603897084No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT33800.17018277025326015No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC32900.1656512763707769No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT32780.1650470771864458No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA32090.16157293187654195No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCT31990.16106943255626605No Hit
GTCAGAGTGTTAGCAGCTTCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG31140.15678968833392076No Hit
GTCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACC30860.15537989023714818No Hit
CCGTAGTACTGGTAAACATCTCTACTAGATCCCCCTGGGCCATCTCTCGC29990.1509994461507477No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG29730.1496903479180303No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT29250.14727355118070592No Hit
TCTATGATGCATCCAACAGGGCCATAGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT28870.1453602537636574No Hit
CTCCTGTGCAGCCTCTGGATTCACGTTTCGGAACTATTGGATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGG28450.14324555661849855No Hit
CTGCTACTCTGGCTCCCAGATACCTCCGGAGAAATTGTGTTGACACAGTC28410.1430441568903882No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG27940.1406777100850914No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC27180.13685111525099442No Hit
CAGTAATATATGGCCGTGTCGTCGGCTCTCAGGCTGTTCAGTTGCAGAAA27060.1362469160666633No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26750.13468606817380796No Hit
GCGTAGCAACTGGCCTCCGTTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT26410.13297417048486984No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACTCATCCAATAGTTCC26290.13236997130053874No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGAGAGAGAGC26210.131967171844318No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG26130.1315643723880973No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG25770.12975177483510397No Hit
GTGTTAGCAGCTTCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCC25770.12975177483510397No Hit
CTCCAGGAGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGC25750.1296510749710488No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25200.12688182870953124No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC24910.1254216806807311No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA24850.12511958108856552No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT24460.12315593373948945No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC24450.12310558380746187No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA24390.1228034842152963No Hit
CATCTATGATGCATCCAACAGGGCCATAGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA24180.12174613564271689No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG24130.12149438598257892No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA23970.12068878707013746No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC23820.11993353808972358No Hit
ACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCATAGGCATCCCAGCCAGGTT23320.11741604148834399No Hit
CAACAGGGCCATAGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG23300.11731534162428882No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC23250.11706359196415085No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23170.11666079250793011No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT22920.11540204420724032No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA22490.11323699713005388No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG22400.11278384774180555No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGCGATCAGCACTGAGC21940.11046775086853633No Hit
GAGTGTTAGCAGCTTCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC21930.11041740093650873No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC21730.1094104022959569No Hit
CAGTAATAAACTGCAAAGTCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA21680.10915865263581893No Hit
CTGCAGGAGAGGGTGGCTCTCTCTCCTGGAGACAAAGACAGGGTGGCTGG21580.10865515331554303No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT21560.10855445345148784No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA21440.10795025426715674No Hit
GCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCTATGG21420.10784955440310157No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG21190.10669150596646694No Hit
CCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCTCCGGAGAAATTGTGTTGACACAGTCTC21180.10664115603443934No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC21140.10643975630632899No Hit
TCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGGC21020.10583555712199788No Hit
CCCAGATACCTCCGGAGAAATTGTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGAGAGAGAGCCAC20970.10558380746185991No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT20870.105080308141584No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC20830.10487890841347364No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA20830.10487890841347364No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT20760.1045264588892805No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG20710.10427470922914255No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT20610.10377120990886662No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT20590.10367051004481144No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC20490.10316701072453552No Hit
ATATTACTGTGCGAGAGATGGCCCAGGGGGATCTAGTAGAGATGTTTACCAGTACTACGGTATGGACATCTGGGG20370.10256281154020443No Hit
GAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTC20260.1020089622879009No Hit
GCCATATATTACTGTGCGAGAGATGGCCCAGGGGGATCTAGTAGAGATGTTTACCAGTACTACGGTATGGACAT20230.10185791249181814No Hit
GGGCTGGAGTGGGTGGCCAACGTAAACCAAGATGGAAGTGAGAAATACTA19980.10059916419112834No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGCGATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAG19940.10039776446301799No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)