FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1986100
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT88770.4469563466089321No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCT67690.34081868989476866No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC64950.3270228085192085No Hit
GTAATATATGGCCGTGTCGTCGGCTCTCAGGCTGTTCAGTTGCAGAAACA58950.29681284930265345No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC56910.28654146316902473No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC54360.27370223050198883No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGAGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG53300.26836513770706405No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGACT52710.2653944917174362No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG50680.2551734555158351No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT49480.24913146367252403No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCTATGGCCCTGTT47210.2377020291022607No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTTCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT44350.22330194854236948No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC43010.21655505765067218No Hit
GCCTAGAGCCTGAAGACTTTGCAGTTTATTACTGTCAGCAGCGTAGCAAC42830.21564875887417553No Hit
ATATATTACTGTGCGAGAGATGGCCCAGGGGGATCTAGTAGAGATGTTTA42470.21383616132118222No Hit
GTTTATTACTGTCAGCAGCGTAGCAACTGGCCTCCGTTCACTTTCGGCCC42440.21368511152509945No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGAACGGAGGCCAGTTGCTACGCTG40770.20527667287649162No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA39990.20134937817833945No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG38660.19465283721866977No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT38620.19445143749055938No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT37560.18911434469563465No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT37430.18845979557927597No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC36980.18619404863803435No Hit
GTAATAAACTGCAAAGTCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA36300.18277025326015808No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36260.18256885353204774No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT35320.1778359599214541No Hit
GTACTACGGTATGGACATCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGT35110.17677861134887468No Hit
GTCTTTGTCTCCAGGAGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTTCTTAGCCTGGTA35100.17672826141684708No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG34740.1749156638638538No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGACTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGG33930.17083731936961885No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG33920.17078696943759125No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC33410.1682191229041841No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG33300.16766527365188055No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC33040.1663561754191632No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCT32240.16232818085695583No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA32140.16182468153667992No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30930.15573233976134132No Hit
GTCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACC30910.15563163989728612No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT29720.14963999798600272No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT29470.14838124968531294No Hit
GTCAGAGTGTTAGCAGCTTCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG29120.14661900206434722No Hit
CTCCTGTGCAGCCTCTGGATTCACGTTTCGGAACTATTGGATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGG29040.14621620260812648No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC28750.1447560545793263No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT27960.14077840994914656No Hit
CTGCTACTCTGGCTCCCAGATACCTCCGGAGAAATTGTGTTGACACAGTC27130.13659936559085645No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGAGAGAGAGC26700.13443431851367No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA26260.13221892150445597No Hit
GTGTTAGCAGCTTCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCC26180.13181612204823523No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACTCATCCAATAGTTCC26060.13121192286390412No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG26020.13101052313579375No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG25950.13065807361160062No Hit
TCTATGATGCATCCAACAGGGCCATAGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT25610.12894617592266253No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG25560.12869442626252456No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA25290.12733497809777958No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG25270.12723427823372438No Hit
CCGTAGTACTGGTAAACATCTCTACTAGATCCCCCTGGGCCATCTCTCGC25270.12723427823372438No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA25170.12673077891344847No Hit
GCGTAGCAACTGGCCTCCGTTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT25050.12612657972911737No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24850.12511958108856552No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC24420.1229545340113791No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA24190.12179648557474447No Hit
CAGTAATATATGGCCGTGTCGTCGGCTCTCAGGCTGTTCAGTTGCAGAAA23910.1203866874779719No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG23640.11902723931322692No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT23490.11827199033281305No Hit
CTCCAGGAGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGC22800.11479784502290921No Hit
CATCTATGATGCATCCAACAGGGCCATAGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA22560.11358944665424701No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA22520.11338804692613665No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA22300.11228034842152962No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22090.11122299984895019No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC21850.11001460148028801No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC21660.10905795277176375No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21570.10860480338351544No Hit
GGGCTGGAGTGGGTGGCCAACGTAAACCAAGATGGAAGTGAGAAATACTA21480.10815165399526712No Hit
CAACAGGGCCATAGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG21360.10754745481093601No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG21070.10608730678213585No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC20850.10497960827752884No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT20790.10467750868536328No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGCGATCAGCACTGAGC20730.10437540909319773No Hit
ACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCATAGGCATCCCAGCCAGGTT20680.10412365943305976No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT20680.10412365943305976No Hit
GAGTGTTAGCAGCTTCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC20490.10316701072453552No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC20380.10261316147223201No Hit
GCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCTATGG20350.10246211167614923No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT20320.10231106188006646No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA20300.10221036201601129No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG20290.10216001208398369No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC20230.10185791249181814No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC20180.10160616283168017No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC20160.101505462967625No Hit
GAGTTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT19930.10034741453099037No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)