FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1299876
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG37000.28464253513412047No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA26000.20001907874289548No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT25190.19378771513590526No Hit
ATAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA25130.1933261326464986No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG23890.1837867611987605No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT23730.18255587456034267No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC21230.1633232708350643No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT19890.15301459523831504No Hit
CTCTAGCCCCTTCCCTGGCGCCTGGCGGACCCAGCTCATGGCAAAGTTGC17550.13501287815145444No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17260.13278189611932215No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC17080.1313971486511021No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC16930.1302431924275854No Hit
CAATAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA16890.12993547076798095No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAACTTTGCCATGAGCTGGG15930.12255015093747404No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC15860.12201163803316625No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT15120.11631878733048384No Hit
CCCTGAGGAGGCGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGCGGA14990.11531869193676936No Hit
GGGCTAGAGTGGGTCTCATCTATTGGTGCTAGTGGTGGTAACACAGACTA14570.11208761451092258No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC14370.11054900621290031No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC14230.10947198040428471No Hit
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTGCGCAGC13930.10716406795725131No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT13850.10654862463804239No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT13810.10624090297843794No Hit
GTGTTACCACCACTAGCACCAATAGATGAGACCCACTCTAGCCCCTTCCC13500.10385606011650342No Hit
GTATATTATTGTGCGAAACCGTTAGTACCAACTTCTTTCCGCTTTGACTA13480.10370219928670119No Hit
GTCCAGGAGTGTGTATTGAATGTATCGTAAATCAGTGTCCTGGGGGCTTG13350.1027021038929867No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT13320.10247131264828338No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC13130.10100963476516221No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC13060.10047112186085441No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2150.041.78497341
TCGTATG2400.038.7886140
AGGGGGG3600.038.42347370
TCTCGTA2250.036.6699738
GGTATCA9700.036.0943031
CGAGACT2750.035.8416823
GTATCAA25200.034.396391
GCTAATC2400.034.16662633
GGATGGG15500.033.66842370
ACGAGAC3050.032.3100522
TATCAAC26950.032.272842
TATACAC3400.031.9762133
ATCAACG27650.031.5786783
CAGTAGG6200.031.43738670
AATGGTA8800.031.43738470
ATGCCGT2900.031.13443644
TCAACGC28050.031.1283574
TATGCCG2900.031.08920543
CAACGCA28250.031.0282465
ATCTCGT2700.030.50843237
Produced by FastQC (version 0.11.5)