FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1299876
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG36710.2824115531019882No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28310.21779000458505274No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA26720.20555806861577564No Hit
ATAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA24320.18709476903950836No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG21790.16763137406952663No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT21220.16324634042016317No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC20450.1573226984727774No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20310.1562456726641618No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT18510.14239819798196135No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT17830.13716692976868564No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC17130.13178180072560766No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16960.13047398367228874No Hit
CTCTAGCCCCTTCCCTGGCGCCTGGCGGACCCAGCTCATGGCAAAGTTGC16750.12885844495936535No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC16720.12862765371466203No Hit
CAATAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA16160.12431955048019966No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT15820.12170391637356177No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC15400.11847283894771501No Hit
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTGCGCAGC15070.11593413525597827No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAACTTTGCCATGAGCTGGG14320.11016435413839475No Hit
CCCTGAGGAGGCGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGCGGA14250.10962584123408695No Hit
GTGTTACCACCACTAGCACCAATAGATGAGACCCACTCTAGCCCCTTCCC13520.10400992094630565No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT13350.1027021038929867No Hit
GGGTTCCCTGGCCCCAGTAGTCAAAGCGGAAAGAAGTTGGTACTAACGGTTTCGCACAATAATATACGGCCGTGT13210.10162507808437113No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC13120.1009327043502611No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12350.040.1249851
GGATGGG15000.036.62266570
GTATCAA29750.035.3674471
CGTTCGT400.002828803433.9021538
TATCAAC32800.031.9555282
CGGAATA550.004266870731.17253569
ATCAACG33500.030.9685363
TCAACGC33750.030.7379554
CGGCAAA850.004812781730.41328670
CAACGCA34150.030.377925
AACGCAG34800.029.8093686
CAGTAGG5950.029.32709970
CGCAGAG36100.028.830928
AGAGTAC36450.028.64598711
GTGGTAT4150.028.6252271
GTATAGG953.696441E-728.5821821
CAGAGTA37550.027.89603210
GCAGAGT38100.027.4933329
CACCGTT708.498969E-426.62245267
AGGATGG13850.026.30534469
Produced by FastQC (version 0.11.5)