FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1262477
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54830.434304941792999No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42410.3359269119358214No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37820.29956981394512533No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37700.2986193015793555No Hit
GCATAATAGTAACCCGTGGACGTTCGGCCAAGGGACCAAGGTGGCAATCAAACGAACTGTGGCTGCACCAT31560.2499847521974658No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30980.2453906090962449No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30600.24238065327130712No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30300.24000437235688252No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29460.2333507857964937No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28830.22836059587620208No Hit
CTATTATGCTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATCGTGAGAGTGAACTCTGTC28030.2220238467710699No Hit
GATCTATGGTGTATCCATTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAGTTCA27740.21972677522045947No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26300.20832062683122146No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25160.19929075935640805No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25070.19857787508208072No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24320.19263717279601927No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24320.19263717279601927No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24140.1912114042473645No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG24060.1905777293368513No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22890.1813102337705954No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22770.1803597214048256No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22340.17695371876081703No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC21530.17053776029187065No Hit
ATAGTAACCCGTGGACGTTCGGCCAAGGGACCAAGGTGGCAATCAAACGA21470.17006250410898574No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20580.16301287072952617No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20110.159290030630261No Hit
CCATAGATCAGGCGCTCAGGGGCTTTCCCTGGTTTCTGCTGATACCAGCC19720.15620086544150905No Hit
GGTTACTATTATGCTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATCGTGAGAGTGAACT18580.14717099796669564No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT18340.14526997323515595No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17370.13758666494518315No Hit
GTATGATAATCTCCCTCTCACTTTCGGCGGAGGGACCACGGTTGAGATCAAACGAACTGTGGCTGCACCAT17140.13576484957745766No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16980.13449749975643122No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16710.1323588469334491No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16670.13204200947819247No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG16660.13196280011437833No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16000.12673498210264425No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15790.12507158546254704No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15040.11913088317648558No Hit
ATATTACTGTCATCAGTATGATAATCTCCCTCTCACTTTCGGCGGAGGGA14990.11873483635741482No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC14710.11651697417061856No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14640.11596250862391949No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14400.11406148389237981No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14370.11382385580093737No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14180.11231887788846846No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTACTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTCCCA14080.11152678425032693No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14070.11144757488651279No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13990.11081389997599955No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13940.11041785315692881No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13770.1090712939720882No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13580.10756631605961929No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG13550.10732868796817685No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13520.10709105987673438No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTT13490.10685343178529193No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13450.10653659433003532No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGACATTAGAAATGATTTAGGCTGGTATCAGCAGAAA13120.10392268532416829No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12950.10257612613932768No Hit
GAGTTCACTCTCACGATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCTACAGCATAATAGTAAC12930.10241770741169938No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGGCATTGGCAACAATTTAAATTGGTATCATCAGAAA12880.10202166059262861No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12790.10130877631830124No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCGC200.007195424352.04533443
GTGGTAT2850.040.466921
GTATCAA27800.040.2656971
AATGGTA7850.039.92968870
CGATCTT501.5071518E-435.76171559
TATCAAC31100.035.6461332
ATCAACG32250.034.2644773
TCAACGC32500.033.9982074
CAACGCA33500.032.9833345
AACGCAG33850.032.539596
ACGCAGA35450.031.166557
GAGTTTA4700.031.0339241
AACTAGA553.54887E-430.90818827
AGAGTAC35450.030.87729511
CGCAGAG35650.030.70658
CAGAGTA35900.030.39344410
AGTTTAT5250.027.7673322
GCAGAGT40100.027.550299
GCGTCTC500.008433809527.11513312
CGAAACC650.00869338226.94652469
Produced by FastQC (version 0.11.5)