FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1708813
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC93050.54453003342086No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT83870.4908085320043797No Hit
CTACAAACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACG83370.48788252430195694No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC67620.3957132816756427No Hit
GAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT65510.383365529171419No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA62050.3631175558706541No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC61910.3622982737139757No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC59850.3502431219799943No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG57710.33771980901362525No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG52250.3057678049031696No Hit
GTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTT50570.2959364190230295No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG49350.2887969602291181No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT49120.28745099668600366No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG48190.2820086223594975No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT47890.2802530177380439No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTTCTG46760.27364024033056866No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA46550.2724113170955511No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT43210.25286558564336764No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG39530.23133016895353675No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG38260.22389810938938318No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACT37540.21968465829789452No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT37050.21681717074952025No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT36410.21307188089041926No Hit
GGTCTAGTCAGAGCCTTCTGCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC35350.20686874456128318No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35110.2054642608641203No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC34940.2044694182452966No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG34490.20183601131311618No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG33240.19452099205705947No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT32770.19177054481678216No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA31920.18679633172266363No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG31710.1855674084876461No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA30770.18006651400709145No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC30700.17965687292875232No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG30350.17760866753705642No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT30240.17696494584252342No Hit
GTTTATTACTGCATGCAAGCTCTACAAACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA29290.17140553120792035No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT29280.1713470110538719No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT28570.1671920801164317No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCC28290.165553515803075No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG28110.16450015303020282No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA28010.1639149514897183No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC27780.16256898794660388No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC27640.1617497057899255No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT27350.16005262132252038No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT27020.1581214562389214No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG26860.15718513377414614No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG26520.15519544853649872No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG26500.15507840822840183No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC26040.15238648114217296No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA25760.15074791682881628No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC25600.149811594364041No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG25590.14975307420999256No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG24900.14571518358064928No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA24420.1429062161863235No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC24100.141033571256773No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT24060.14079949064057917No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA24030.1406239301784338No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC24010.14050688987033688No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG23780.13916092632722246No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT22840.13366003184666783No Hit
ATCCATTACTATGCAGAAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC22830.13360151169261938No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC22770.13325039076832867No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA22660.13260666907379567No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA22590.1321970279954565No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACTCCGCTCACTTTCGGCGGAGGGA22450.13137774583877815No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC22340.13073402414424515No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA22050.12903693967684No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC22040.12897841952279154No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC21920.1282761776742101No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC21890.12810061721206475No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21740.12722281490133794No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA21480.12570129089607815No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT21360.12499904904749672No Hit
TTATTACTGCATGCAAGCTCTACAAACTCCGCTCACTTTCGGCGGAGGGA21130.12365308550438228No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA21000.12289232350175239No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA20180.11809367086977919No Hit
GTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAAC19630.11487506239711426No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT19620.1148165422430658No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA19510.1141728205485328No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC19390.11347057869995136No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGG19380.11341205854590292No Hit
GGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAAT19220.11247573608112765No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18990.11112977253801323No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT18980.11107125238396477No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT18860.11036901053538334No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA18740.1096667686868019No Hit
GCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC18710.10949120822465652No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCATTACTATGC18640.10908156714631737No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA18350.1073844826789122No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC17930.10492663620887716No Hit
GCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTT17830.10434143466839263No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC17760.10393179359005345No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT17590.10293695097122973No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA17460.10217618896859984No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT17410.10188358819835756No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGA17200.10065466496334005No Hit
CTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCT17160.10042058434714624No Hit
GGTTTATTACTGCATGCAAGCTCTACAAACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA17100.10006946342285553No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)