FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132026
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT20221.531516519473437No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGT9020.6831987638798418No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4620.349931074182358No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4430.33553996939996666No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3910.29615378789026403No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3850.29160922848529836No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC3640.27570327056791843No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3200.24237650159817004No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3070.2325299562207444No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2800.21207943889839878No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2730.20677745292593883No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2670.20223289352097315No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2450.18556950903609895No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2360.1787526699286504No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2310.174965537091179No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2260.17117840425370762No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2230.16890612455122475No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2130.16133185887628193No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2070.15678729947131625No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2070.15678729947131625No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2020.15300016663384483No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1990.15072788693136202No Hit
TCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCG1980.1499704603638677No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1830.1386090618514535No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1780.1348219290139821No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1770.13406450244648782No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1740.13179222274400496No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1720.13027736960901642No Hit
CTCCAGCCCCCACGTTGCACCAGACCCGTACTCTGCGTTGATAC1610.12194567736657931No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1590.12043082423159074No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1560.1181585445291079No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1550.11740111796161362No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1500.11361398512414221No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1490.11285655855664795No Hit
GTATCAACGCAGAGTACGGGTCTGGTGCAACGTGGGGGCTGGAG1470.11134170542165937No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCC1450.10982685228667081No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1400.10603971944919939No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1400.10603971944919939No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1390.10528229288170513No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1380.10452486631421085No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1350.102252586611728No Hit
GTATCAACGCAGAGTACGGGTCTGGTGCAACGTGGGGGCTGGAGGGTGGG1350.102252586611728No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3650.0135.7139470
AAGGGGG4050.085.1802569
CGTCTTG203.0335239E-584.8826248
AAAGGGG4300.072.2419868
GCTTGAA5000.070.96187656
CTTGAAA5150.069.5553657
AAAAGGG4500.067.7946567
TGCTTGA5100.067.75452455
GATAGGG150.00268901366.6934936
CTGCTTG5050.065.03837654
CTTCTGC4850.064.19913551
GTCTTAT200.003136312464.1417449
CGTCTTA200.003231104763.6619748
TTCTGCT4850.063.2556852
TCTGCTT5100.062.65385453
TTGAAAA5900.062.26841458
TCTTCTG5050.061.97233650
AAAAAGG4900.061.08736866
CGTCCTA200.003896050660.72694844
GTCTTCT5000.060.72084849
Produced by FastQC (version 0.11.5)