FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132026
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50633.8348507112235466No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4570.34614394134488663No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4250.3219062911850696No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4250.3219062911850696No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3450.26131216578552713No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3440.26055473921803285No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2810.21283686546589306No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2510.19011406844106463No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2490.1885992153060761No Hit
GTATCAACGCAGAGTACGGGTCTGGTGCAACGTGGGGGCTGGAGGGTGGG2480.1878417887385818No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2330.17648039022616757No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2310.174965537091179No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2290.17345068395619043No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2110.1598170057412934No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2110.1598170057412934No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1980.1499704603638677No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1950.14769818066138488No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1940.1469407540938906No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1780.1348219290139821No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1770.13406450244648782No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1740.13179222274400496No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1740.13179222274400496No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1730.1310347961765107No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1680.12724766333903928No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1680.12724766333903928No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1620.12270310393407359No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1610.12194567736657931No Hit
GTATCAACGCAGAGTACGGGTCTGGTGCAACGTGGGGGCTGGAG1600.12118825079908502No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1580.11967339766409647No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1560.1181585445291079No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1560.1181585445291079No Hit
CTCCAGCCCCCACGTTGCACCAGACCCGTACTCTGCGTTGATAC1440.10906942571917652No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTT1430.10831199915168224No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTATG200.00419587759.6023644
CAGTATC150.005039661756.9191971
CAAAGCA150.00505482156.8760455
GGAGGCG652.5941184E-454.9593470
TCTTTAT356.418354E-445.7994545
CGGGTCC256.576719E-445.58724217
CAACGAA256.6385296E-445.5008355
TCTAATG400.001149923740.68302547
CAGTGTA300.001577020738.16329621
GCAGTGT300.001588889338.10514520
TTCTTTA500.003859868331.78792644
AAAAGGT650.00475613830.45240664
CTAATGT550.005224726629.87931648
AGTGTAT400.006395787528.6771822
CGGGGCA505.688736E-428.49202517
TGTCGAC450.00704486328.11298431
GTTAAAA650.00861946226.9660354
CACCCCG1405.456968E-1226.39672911
GGCTGCA559.796895E-425.96099919
GAAAAGA559.950362E-425.89200616
Produced by FastQC (version 0.11.5)