FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences985046
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43750.4441416949056186No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31090.3156197781626442No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26300.26699260745183473No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24850.2522724827063914No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24600.24973453016407357No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21090.21410167646993133No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA20400.20709692745313418No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18910.19197073030091996No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18830.19115858548737827No Hit
ACATAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCACCAGGGCCACTGGCATCCCAGCCAGGTT18580.18862063294506043No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17670.17938248569102358No Hit
GGTTTATGTTGGTACCAGGCTACAAATTCACTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCC16910.17166710996237738No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16470.16720031348789802No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT16120.16364717992865307No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGGT15690.1592819015558664No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT15270.15501814128477248No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14840.15065286291198585No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14580.1480133922679753No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14420.14638910264089192No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14360.1457799940307356No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14110.1432420414884178No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14020.14232837857318337No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13910.14121167945456353No Hit
GTTTATTACTGTCAGCAGCGTGTCAACTGGTTGCTCACTTTCGGCGGCGGGACCAAGGTGGAGATCAAACGAA13790.139993462234251No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13720.139282835522402No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTGAATTTGTAGCCTGGTACCAACATAAACCTGGCCAGGCTCCCAGGCT13270.13471452094622993No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13140.13339478562422466No Hit
GCCTAGAGCCTGAAGATTTTGCGGTTTATTACTGTCAGCAGCGTGTCAACTGGTTGCTCACTTTCGGCGGCGGGA13110.13309023131914652No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCGCCGCCGAAAGTG13080.13278567701406838No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTGAATTTGTAGCCTGGTA12560.12750673572604732No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12250.1243596745735732No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC11330.11502000921784364No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCGGTTTA11050.11217750237044767No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA10900.11065473084505698No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10800.10963954982812986No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10740.10903044121797358No Hit
GTAATAAACCGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA10560.10720311538750474No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10260.10415757233672336No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10130.10283783701471809No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9890.100401402574093No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGCA200.0083530350.11709630
GGTATCA14000.043.499141
GTATCAA32200.034.638971
TCGTGCG400.003173277233.11569220
TATCAAC35900.030.959432
TCAACGC36650.030.2310144
ATCAACG36650.030.1361833
CAACGCA37350.029.8430545
AACGCAG37700.029.5629946
TAGTCAC1302.2555469E-1028.86519438
AGAGTAC39850.027.5474511
ACGCAGA40350.027.539477
CGCAGAG40250.027.5257268
GCAGAGT41100.026.8746269
AATGGTA5200.026.8675570
GAGTTTA3200.025.8571661
CGTGTAA1759.822543E-1125.41919758
ATACGTA1451.1514203E-925.08397311
TCTCGCG3101.3169483E-924.78748570
GGTAATC1551.5797923E-824.3428161
Produced by FastQC (version 0.11.5)