FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences889672
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC34950.39284140672067913No Hit
GTAATACACAGCCGTGTCCTCGGGTGTCAGGCTGGTCATTTGCAGAAACA29780.3347301027794513No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG29130.3274240394212699No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24080.27066154717693713No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23690.26627790916202826No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT22830.2566114253342805No Hit
GTGTATTACTGCGCGAGAGACTATGATCCTACTGGTTATTATTTAGGCGG22600.2540262029152317No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21520.2418868976431764No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20900.23491803720921867No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATGACTTTGTCTCCTGGTACCAACTACA17940.2016473486858078No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17740.19939932919098274No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17010.1911940580348713No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC16860.1895080434137525No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG16850.18939564243901122No Hit
GCCTTGGGCTGACTGAGGACGGTCAGTTTGGTCCCCCCGCCGAAAAGCACAGTGCTCCAGGATGTATATGAGGTG16350.18377559370194857No Hit
GATTATTACTGCACCTCATATACATCCTGGAGCACTGTGCTTTTCGGCGGGGGGACCAAACTGACCGTCCTCAGT15930.17905475276281596No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15580.1751207186468721No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15470.17388430792471832No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15230.17118668453092825No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15010.16871386308662067No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14870.16714024944024317No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14730.1655666357938656No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14730.1655666357938656No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14490.16286901240007554No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT14100.15848537438516666No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT14080.15826057243568417No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTGAGGACGGTCAGTTTGGTCCCCC14000.15736136463775413No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13810.15522574611767034No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13630.15320252857232777No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13560.152415721749139No Hit
GTAATAATCAGCCTCGTCCTCAGGCTGGAGCCCAGAGATGGTCAGGGAGG13420.15084210810276147No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG13410.15072970712802022No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13110.14735767788578263No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12660.14229963402242624No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12650.142187233047685No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC12460.14005161452760118No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11890.13364475896734976No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC11830.13297035311890223No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11790.13252074921993723No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC11400.12813711120502835No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATGACTTTGTCTCCTGGTACCAACTACACCCAGGCAAGGC11370.12779990828080462No Hit
CAGTAATACACAGCCGTGTCCTCGGGTGTCAGGCTGGTCATTTGCAGAAA11240.12633869560916833No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11230.12622629463442706No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC11210.12600149268494457No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10800.12139305272055319No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC10780.12116825077107067No Hit
CCTCATATACATCCTGGAGCACTGTGCTTTTCGGCGGGGGGACCAAACTGACCGTCCTCAGTCAGCCCAAGGCTG10700.12026904297314067No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10690.1201566419983994No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT10600.11914503322572813No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10420.11712181568038559No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10390.11678461275616181No Hit
TCTCTGGGCTCCAGCCTGAGGACGAGGCTGATTATTACTGCACCTCATAT10360.11644740983193806No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10350.11633500885719682No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC10290.1156606030087493No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10220.11487379618556053No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10180.11442419228659552No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG9820.1103777571959104No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA9810.11026535622116916No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9800.11015295524642789No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9730.10936614842323913No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9710.10914134647375662No Hit
GTATATGAGGTGCAGTAATAATCAGCCTCGTCCTCAGGCTGGAGCCCAGA9660.10857934160005037No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9530.10711812892841407No Hit
GTGCTCCAGGATGTATATGAGGTGCAGTAATAATCAGCCTCGTCCTCAGG9500.1067809260041903No Hit
CATGTATACTACTCAGACTCACTGAAGGGCCGATTCACCATTTCCAGAGACAACGCCAAGAACTCACTGTTTCTG9470.10644372307996655No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9360.10520731235781278No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT9340.10498251040833026No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9300.10453290650936525No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC9280.10430810455988274No Hit
CATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC9000.10116087726712766No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT8990.10104847629238642No Hit
GCCTTGGGCTGACCTGGGTGTAGTTGGTACCAGGAGACAAAGTCATAACCACCAACGTCACTGCTGGTTCCAGTG8940.10048647141868014No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG8930.1003740704439389No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC8920.10026166946919762No Hit
GTATTACTGCGCGAGAGACTATGATCCTACTGGTTATTATTTAGGCGGGG8910.10014926849445638No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTAGG150.00244378368.361732
CGACGAT200.007012421752.3842350
GTACTAG200.00762539751.2857131
TCGTATG1350.048.60910840
GGTATCA5550.044.3552131
CGTTCGA551.3934368E-744.20606645
TCTGGAA9350.043.7567970
TCTCGTA1400.041.8629838
GTATCAA16700.041.765611
ACGTTCG602.7907117E-740.4644544
AGGGGGG2450.040.4008670
CCCTAAG1750.039.11661528
GTGGTAT1400.039.074831
CGAGCCC1750.039.0638515
GCCGTCT1700.038.85067446
TAAGACA1800.038.08791731
GTCGTCG654.6180503E-737.94695356
CCGTCTT1750.037.77952647
TCCGAGC1900.037.7788613
ATCTCGT1550.037.7753237
Produced by FastQC (version 0.11.5)