FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871008
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGGATGGGATGCCAGCCTGAATGGCTGGGTGTTCGG66630.7649757522318968No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG62010.7119337595062273No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC47980.5508560196921268No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGCCATTCAGGCTGGCATCCCATC36360.41744737132150334No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGCGACCC31650.3633720930232558No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG31390.3603870458135861No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGCCATTCAGG31090.3569427605716595No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30250.34729876189426506No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT29740.3414434769829898No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT26760.3072302435798523No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA26710.3066561960395312No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26470.3039007678459899No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC24600.28243138983798083No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT24470.28093886623314596No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT22950.26348782100738455No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC22770.2614212498622286No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22680.2603879642896506No Hit
GGATGGGATGCCAGCCTGAATGGCTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG20910.2400666813622837No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG20830.23914820529776995No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20610.2366223961203571No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC20470.23501506300745803No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20060.23030787317682502No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG19920.22870054006392596No Hit
CCTCAGGGGTCCCTGACCGATTTTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT19860.22801168301554062No Hit
GTGCAGGATGGGATGCCAGCCTGAATGGCTGGGTGTTCGGCGGAGGGACC19850.22789687350747637No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC19660.2257154928542562No Hit
CCCCTGAGGGCCGCTGATTATTTTTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTCA19480.22364892170910028No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGCGACCCCCGGGCAGAGGGTCGC19060.21882692237040302No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19060.21882692237040302No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG18910.21710477974943976No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18210.20906811418494434No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGGATGGGATGCCAGCCTGAATGGCTGGGTGTTCGGCGGAGGGAC17800.20436092435431133No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17460.20045740108012786No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16720.1919614974833756No Hit
ATCCTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATG16190.18587659355597194No Hit
CCCTCAGCGTCTGCGACCCCCGGGCAGAGGGTCGCGATCTCTTGTTCTGGAAGCAGCTCCAACATCGAAAGAAAT16150.18541735552371505No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAAAATCGGTCAGGGACC15950.18312116536243064No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGCCATTCAGGC15700.18025092766082515No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC15490.17783992799147655No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15430.17715107094309124No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14650.16819592931408206No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT14610.1677366912818252No Hit
ATCCCATCCTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCAC14530.16681821521731144No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC14530.16681821521731144No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14490.16635897718505455No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13880.15935559719313716No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAG13860.15912597817700871No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGAAAGAAATCATGTGAACTGGTACCAGCAGCTCCCAGGAACGGCCCC13820.15866674014475182No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT13570.15579650244314633No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC13570.15579650244314633No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13260.15223740769315552No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13250.1521225981850913No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT13110.1505152650721922No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13030.14959678900767845No Hit
GTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTCACATGATTTCTT12990.14913755097542156No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG12890.14798945589477938No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12830.14730059884639407No Hit
GCTGGTACCAGTTCACATGATTTCTTTCGATGTTGGAGCTGCTTCCAGAA12820.14718578933832985No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAAAATCGGTC12630.14500440868510966No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC12420.14259340901576106No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12310.14133050442705464No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC11910.13673812410448583No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11250.12916069657224732No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA11240.12904588706418313No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10840.12445350674161432No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC10610.12181288805613724No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG10480.1203203644513024No Hit
GCCCTCAGGGGTCCCTGACCGATTTTCTGGCTCCAAGTCTGGCACCTCAG10410.11951669789485286No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG10360.11894265035453176No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC10310.11836860281421067No Hit
GTGCCAGACTTGGAGCCAGAAAATCGGTCAGGGACCCCTGAGGGCCGCTG10220.1173353172416327No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG10190.11699088871744003No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10110.11607241265292627No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC9860.11320217495132077No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9790.11239850839487123No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT9680.11113560380616481No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG9660.11090598479003638No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG9640.11067636577390794No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC9630.11056155626584371No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC9560.10975788970939418No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9520.10929865167713729No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC9440.10838017561262353No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTATAAAAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTTTCTGG9420.10815055659649508No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGCGACCCCCGGGCAGAGG9390.10780612807230244No Hit
GCTTGGTCCCTCCGCCGAACACCCAGCCATTCAGGCTGGCATCCCATCCTGCACAGTAATAATCAGCCTCATCCT9350.10734689004004555No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA9300.10677284249972446No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC9150.10505069987876116No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC8930.10252489070134833No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8880.10195084316102722No Hit
GAACAAGAGATCGCGACCCTCTGCCCGGGGGTCGCAGACGCTGAGGGTGG8830.10137679562070613No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG8810.10114717660457768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1350.053.3213921
CTCGTTT200.007558915351.3993514
AACGCAC200.007558915351.3993512
GGTATCA6100.046.078681
AGGGGGG1700.045.2555970
CGTATGC1300.042.5204541
TCGTATG1300.042.4884840
AATGGTA5000.039.7494970
AATCTCG1300.039.7089336
GTATCAA17050.039.002581
TGGTATC2000.037.6950232
TTATACA1550.037.5844762
TATCAAC17950.036.845472
GCTAATC1400.036.8110333
AGCTAAT1600.036.4937732
ACGAGAC1700.036.28189522
TCTCCGA1700.036.27980811
TCTCGTA1450.035.6545638
CTTATAC1650.035.316771
TATACAC1650.035.30463
Produced by FastQC (version 0.11.5)