FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004691692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871008
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGGATGGGATGCCAGCCTGAATGGCTGGGTGTTCGG64020.7350104706271354No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC45230.5192834049744663No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG39580.45441603291818217No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGCGACCC36510.41916951394246665No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32100.3685385208861457No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGCCATTCAGGCTGGCATCCCATC30340.34833204746684304No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT29440.33799919174106324No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28050.3220406701201367No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27620.31710386127337525No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27450.3151520996362835No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGCCATTCAGG25660.29460119769278814No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24710.28369429442668725No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT24070.27634648591057714No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA23890.2742799147654212No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC23720.2723281531283295No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC23040.26452110657996253No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGCGACCCCCGGGCAGAGGGTCGC22930.2632582019912561No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22510.2584362026525589No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG22250.25545115544288916No Hit
GGATGGGATGCCAGCCTGAATGGCTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG22000.25258091774128366No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21850.25085877512032034No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC20370.23386696792681586No Hit
CCCTCAGCGTCTGCGACCCCCGGGCAGAGGGTCGCGATCTCTTGTTCTGGAAGCAGCTCCAACATCGAAAGAAAT19450.2233044931849076No Hit
GTGCAGGATGGGATGCCAGCCTGAATGGCTGGGTGTTCGGCGGAGGGACC19430.22307487416877916No Hit
CCTCAGGGGTCCCTGACCGATTTTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT19380.22250082662845805No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19350.2221563981042654No Hit
CCCCTGAGGGCCGCTGATTATTTTTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTCA18880.21676035122524706No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG18690.2145789705720269No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGGATGGGATGCCAGCCTGAATGGCTGGGTGTTCGGCGGAGGGAC18480.21216797090267825No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17690.2030980197656049No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17570.20172030566883428No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17490.2008018296043205No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG17400.19976854403174252No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC16940.19448730666078842No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16650.19115783092692606No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT16370.1879431647011279No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15980.1834655938866233No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGAAAGAAATCATGTGAACTGGTACCAGCAGCTCCCAGGAACGGCCCC15930.1828915463463022No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC15170.17416602373342152No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14780.16968845291891693No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14760.16945883390278849No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC14400.16532569161247657No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13880.15935559719313716No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC13850.1590111686689445No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT13840.15889635916088027No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAG13790.15832231162055918No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13650.15671497850766009No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13590.15602612145927477No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC13000.14925236048348578No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG12950.1486783129431647No Hit
GTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTCACATGATTTCTT12930.14844869392703625No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12880.14787464638671516No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGCCATTCAGGC12850.1475302178625225No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12800.1469561703222014No Hit
GCTGGTACCAGTTCACATGATTTCTTTCGATGTTGGAGCTGCTTCCAGAA12610.14477478966898125No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT12090.13880469524964179No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA11680.13409750541900878No Hit
ATCCTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATG11520.13226055328998126No Hit
GCCCTCAGGGGTCCCTGACCGATTTTCTGGCTCCAAGTCTGGCACCTCAG11420.13111245820933906No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAAAATCGGTC11400.13088283919321064No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11240.12904588706418313No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10720.12307579264484367No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGCGACCCCCGGGCAGAGG10640.12215731658032991No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10620.12192769756420146No Hit
ATCCCATCCTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCAC10390.11928707887872442No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10360.11894265035453176No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG10270.11790936478195378No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC10230.11745012674969692No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9950.11423546052389875No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTATAAAAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTTTCTGG9910.11377622249164186No Hit
GTGCCAGACTTGGAGCCAGAAAATCGGTCAGGGACCCCTGAGGGCCGCTG9900.11366141298357764No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG9730.11170965134648592No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG9710.11148003233035747No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC9650.11079117528197216No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC9530.10941346118520151No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG9520.10929865167713729No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGCGACCCCCGGGC9470.1087246041368162No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC9460.10860979462875198No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC9420.10815055659649508No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC9380.10769131856423822No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC9370.107576509056174No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGT9160.10516550938682537No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG9060.10401741430618318No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG9040.10378779529005475No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC8960.10286931922554098No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC8950.10275450971747677No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAG8930.10252489070134833No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG8910.10229527168521989No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA8900.10218046217715566No Hit
CTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCT8900.10218046217715566No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)