FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1923773
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAACACTATGCTACCTCATCTCGAACGTTCGGCCAAGGGA85620.445062905030895No Hit
GTAATAGACGGCCGTGTCCTCGACTCTCAGGCTGTTCATGTGGAGATACA73100.379982461548218No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT71250.37036594234350934No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC63020.32758542717877837No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA54740.2845450060895958No Hit
GTCTATTACTGCGCGAAGGATGAGGGCAATGGCTGGTACTACTTTGACAA53970.28054245485304136No Hit
CAGTAATACACTGCAAAATCTTCTGGCTCCAGTTTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA51080.2655198924197398No Hit
GTGTATTACTGTCAACACTATGCTACCTCATCTCGAACGTTCGGCCAAGGGACCAAGCTGGAAATCAAACGAA50510.2625569648809917No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGATGGCTTTTT49290.256215260324373No Hit
ATGCTACCTCATCTCGAACGTTCGGCCAAGGGACCAAGCTGGAAATCAAA48090.24997751813753494No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA46380.24108873552129073No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43260.22487060583551177No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42670.22180371592698309No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG42160.21915267549757692No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC42040.2185289012788931No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT41090.21359068871431297No Hit
GTAATACACTGCAAAATCTTCTGGCTCCAGTTTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA40090.20839257022528127No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG38950.2024667151477851No Hit
CTCCTGTGCAGCCTCTGGATTCACCCTTACCAGCAATCCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG38440.19981567471837894No Hit
GTCTCCAGCCACCCTGTGTTTGGCTCCAGGGGAAAGAGCCACCCTCTCCT38430.1997636935334886No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37860.19680076599474053No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAAACTGGAGCCAGAAGATT37380.19430566912000533No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC36740.19097887328702504No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTAAG36190.18811990811805757No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC35570.18489707465485794No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC35340.18370150740238064No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA34530.17949103142626496No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG34430.17897121957736178No Hit
AGGTAGCATAGTGTTGACAGTAATACACTGCAAAATCTTCTGGCTCCAGTTTGCTGATGGTGAGAGTGAAGT33030.1716938536927174No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG32840.17070621117980136No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG32820.17060224881002073No Hit
GTGTTTGGCTCCAGGGGAAAGAGCCACCCTCTCCTGCGGGGCCAGTCAGA32260.16769130245616296No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA32110.1669115846828082No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT31840.16550809269076963No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG30910.16067384249597016No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC30840.16030997420173793No Hit
GTATTAGCGGGAGTGGTAGTTGGACATACCACGCAGACTCCGTGAGGGGCCGTTTCACTTTCTCCAGAGACACTT30350.15776289614211242No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30300.15750299021766082No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30200.15698317836875764No Hit
GTGTTAGCAGTAACTACTTAGCCTGGTACCAGCAGAAACCTGGCCTGGCGCCCAGGCTCCTCATTTATGATGGAT30070.15630742296518355No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC28750.14944590655966167No Hit
GTGCAGCCACAGTTCGTTTGATTTCCAGCTTGGTCCCTTGGCCGAACGTT27570.14331212674260427No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27180.14128486053188186No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG27130.14102495460743028No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG26860.13962146261539174No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT26790.1392575943211595No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG26440.13743825284999842No Hit
CTCCTGCGGGGCCAGTCAGAGTGTTAGCAGTAACTACTTAGCCTGGTACC26420.13733429048021778No Hit
CCTTGGGGCTGGTCGGGGATGTTGCGGAGACGGAGACCAGGGTTCCCTGGCCCCATTTGTCAAAGTAGTACCAGC25940.13483919360548258No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25850.1343713629414697No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC25780.1340074946472375No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGATGGCTTTTTCTTGT25500.1325520214703086No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT25290.13146041658761196No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGATGGC25170.13083664236892814No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25170.13083664236892814No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT24920.1295371127466702No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG24910.12948513156177988No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCAGCTTGGTCC24880.12932918800710894No Hit
GTCAGAGTGTTAGCAGTAACTACTTAGCCTGGTACCAGCAGAAACCTGGC24860.1292252256373283No Hit
CTACTTAGCCTGGTACCAGCAGAAACCTGGCCTGGCGCCCAGGCTCCTCATTTATGATGGATCACTTAGGGCCA24590.12782173364528976No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC24080.1251706932158836No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA23990.12470286255187073No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT23750.12345531411450313No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC23700.12319540819005154No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23560.12246767160158709No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT23550.12241569041669678No Hit
AAGTAGTACCAGCCATTGCCCTCATCCTTCGCGCAGTAATAGACGGCCGT23540.12236370923180645No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG23270.1209602172397679No Hit
GACTTCACTCTCACCATCAGCAAACTGGAGCCAGAAGATTTTGCAGTGTA23260.12090823605487758No Hit
CTCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCCTTACCAGCAATCCC23180.12049238657575503No Hit
GGATGAGGGCAATGGCTGGTACTACTTTGACAAATGGGGCCAGGGAACCC22840.11872502628948425No Hit
CCCTGTATCTCCACATGAACAGCCTGAGAGTCGAGGACACGGCCGTCTAT22710.11804927088591013No Hit
GGGCTGGAGTGGGTCTCAAGTATTAGCGGGAGTGGTAGTTGGACATACCA22590.11742549666722633No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATGGGATTGCTGG21980.114254644388917No Hit
GTACTACTTTGACAAATGGGGCCAGGGAACCCTGGTCTCCGTCTCCGCAACATCCCCGACCAGCCCCAAGGTCTT21970.11420266320402667No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG21930.11399473846446541No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21910.11389077609468476No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT21490.11170756632929145No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGTTGCGGAGACGGAGACCAGGGTTCCCTGG21390.11118775448038828No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21190.11014813078258194No Hit
CTTAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAAA20940.10884860116032401No Hit
GCCTGAGAGTCGAGGACACGGCCGTCTATTACTGCGCGAAGGATGAGGGC20820.1082248269416402No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATCGTGTTGACGCAGTC20750.107860958647408No Hit
GCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGTTGAGGGAACTGGGCAGGGCACAGT20740.10780897746251766No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATCGTGTTGACGCAGTCTC20650.10734114679850482No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20540.10676935376471132No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC20300.10552180532734372No Hit
GCCAAGGGACCAAGCTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC20130.10463812518420833No Hit
CTCCGTGAGGGGCCGTTTCACTTTCTCCAGAGACACTTCCAAGAACACCC20070.10432623807486642No Hit
GAACTGGGCAGGGCACAGTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGG19920.10354652030151167No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT19820.1030267084526085No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT19790.10287076489793755No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19660.10219500949436341No Hit
CAGTAATAGACGGCCGTGTCCTCGACTCTCAGGCTGTTCATGTGGAGATA19640.10209104712458278No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19490.10131132935122804No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT19480.10125934816633772No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)