FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1923773
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAACACTATGCTACCTCATCTCGAACGTTCGGCCAAGGGA80040.41605740386209805No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72050.3745244371347347No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT69020.3587741381129686No Hit
GTAATAGACGGCCGTGTCCTCGACTCTCAGGCTGTTCATGTGGAGATACA66250.34437534989835084No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC53050.2757601858431322No Hit
GTCTATTACTGCGCGAAGGATGAGGGCAATGGCTGGTACTACTTTGACAA52310.2719135781612487No Hit
GTGTATTACTGTCAACACTATGCTACCTCATCTCGAACGTTCGGCCAAGGGACCAAGCTGGAAATCAAACGAA51150.26588376071397196No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA50830.2642203627974818No Hit
CAGTAATACACTGCAAAATCTTCTGGCTCCAGTTTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA47380.24628685401032244No Hit
ATGCTACCTCATCTCGAACGTTCGGCCAAGGGACCAAGCTGGAAATCAAA46300.24067288604216816No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC44880.23329155778774313No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC43340.22528645531463432No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGATGGCTTTTT43060.22383098213770544No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC41520.2158258796645966No Hit
GTAATACACTGCAAAATCTTCTGGCTCCAGTTTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA40190.20891238207418444No Hit
CTCCTGTGCAGCCTCTGGATTCACCCTTACCAGCAATCCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG39700.2063653040145589No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT38800.20168699737443035No Hit
GTCTCCAGCCACCCTGTGTTTGGCTCCAGGGGAAAGAGCCACCCTCTCCT38370.1994518064241467No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT38130.19820425798677913No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38090.19799633324721783No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA37630.19560519874226326No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAAACTGGAGCCAGAAGATT37300.19388981964088278No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG35990.18708028442025126No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35210.1830257519988065No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG34850.18115442934275508No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTAAG34030.1768919721817491No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC32920.1711220606589239No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32910.17107007947403358No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA32860.170810173549582No Hit
GTGTTAGCAGTAACTACTTAGCCTGGTACCAGCAGAAACCTGGCCTGGCGCCCAGGCTCCTCATTTATGATGGAT31810.16535214913609872No Hit
GTGTTTGGCTCCAGGGGAAAGAGCCACCCTCTCCTGCGGGGCCAGTCAGA31680.16467639373252457No Hit
AGGTAGCATAGTGTTGACAGTAATACACTGCAAAATCTTCTGGCTCCAGTTTGCTGATGGTGAGAGTGAAGT31190.16212931567289904No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30950.16088176723553144No Hit
GTATTAGCGGGAGTGGTAGTTGGACATACCACGCAGACTCCGTGAGGGGCCGTTTCACTTTCTCCAGAGACACTT29850.15516383689759655No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT29370.15266874002286132No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28890.15017364314812612No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG28780.1496018501143326No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC28760.14949788774455197No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG28560.14845826404674564No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28000.14554731769288787No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27850.14476759991953314No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT27400.14242844659946885No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27210.14144080408655282No Hit
GTGCAGCCACAGTTCGTTTGATTTCCAGCTTGGTCCCTTGGCCGAACGTT27080.1407650486829787No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26820.13941353787583047No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26620.1383739141780241No Hit
CTCCTGCGGGGCCAGTCAGAGTGTTAGCAGTAACTACTTAGCCTGGTACC26580.13816598943846287No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG26140.1358788173032889No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC25310.1315643789573926No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA25270.13135645421783132No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT25040.13016088696535402No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG24810.12896531971287672No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG24630.128029658384851No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG24570.1277177712755091No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA24490.12730192179638658No Hit
CCTTGGGGCTGGTCGGGGATGTTGCGGAGACGGAGACCAGGGTTCCCTGGCCCCATTTGTCAAAGTAGTACCAGC24480.12724994061149628No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC24050.12501474966121262No Hit
GACTTCACTCTCACCATCAGCAAACTGGAGCCAGAAGATTTTGCAGTGTA23830.12387116359362565No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT23580.12257163397136772No Hit
GTCAGAGTGTTAGCAGTAACTACTTAGCCTGGTACCAGCAGAAACCTGGC23360.12142804790378076No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC23190.12054436776064537No Hit
CTCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCCTTACCAGCAATCCC23100.1200765370966325No Hit
CCCTGTATCTCCACATGAACAGCCTGAGAGTCGAGGACACGGCCGTCTAT22850.11877700747437459No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG22800.118517101549923No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT22730.11815323325569077No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC22590.11742549666722633No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCAGCTTGGTCC22550.11721757192766505No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGATGGC22540.11716559074277474No Hit
GGGCTGGAGTGGGTCTCAAGTATTAGCGGGAGTGGTAGTTGGACATACCA22360.11622992941474904No Hit
GTACTACTTTGACAAATGGGGCCAGGGAACCCTGGTCTCCGTCTCCGCAACATCCCCGACCAGCCCCAAGGTCTT22320.11602200467518778No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGATGGCTTTTTCTTGT21890.11378681372490414No Hit
GGATGAGGGCAATGGCTGGTACTACTTTGACAAATGGGGCCAGGGAACCC21880.1137348325400138No Hit
GCCTGAGAGTCGAGGACACGGCCGTCTATTACTGCGCGAAGGATGAGGGC21810.1133709642457816No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT21610.11233134054797525No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC21600.11227935936308493No Hit
CTACTTAGCCTGGTACCAGCAGAAACCTGGCCTGGCGCCCAGGCTCCTCATTTATGATGGATCACTTAGGGCCA21490.11170756632929145No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATCGTGTTGACGCAGTC21300.11071992381637542No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC21220.1103040743372529No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT21190.11014813078258194No Hit
AAGTAGTACCAGCCATTGCCCTCATCCTTCGCGCAGTAATAGACGGCCGT20990.1091085070847756No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATGGGATTGCTGG20870.10848473286609178No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20720.10770501509273704No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGTTGCGGAGACGGAGACCAGGGTTCCCTGG20490.10650944784025974No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG20090.10443020044464706No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20060.1042742568899761No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGG20030.10411831333530515No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20030.10411831333530515No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATCGTGTTGACGCAGTCTC19960.10375444504107294No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19790.10287076489793755No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)