FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1041358
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29590.2841481987942667No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC22620.2172163655534408No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17690.1698743371635883No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17640.16939419488782917No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16260.15614226807687653No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16090.15450978433929544No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15650.15028453231261488No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT15210.14605928028593435No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14640.14058565834227998No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA14200.13636040631559943No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13480.12944635754466766No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12640.12137996731191388No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGAAGCGGTTGTGCAG12370.11878719902281443No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG12250.11763485756099247No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11910.11436989008583023No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT11760.11292946325855277No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11580.11120095106581981No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11390.10937641041793504No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGTGCCCGGGAAGTATGTA11310.1086081827767204No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10930.10495910148095083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCAT1008.3451596E-847.1775470
GTGGTAT4950.041.0439761
TTGGCGA400.001534680838.39967366
GTATCAA51400.034.926131
CACGCAA450.002846059933.85903565
GGTATCA23300.033.4491231
ATCAACG56000.031.3803373
TCAACGC57050.030.9209484
CAACGCA57550.030.7116135
AACGCAG58400.030.263166
CGCCTAT450.00497464630.20968636
TATCGCC450.005131727630.01824233
TATCAAC60500.029.5442092
CGCAGAG59950.029.371088
AGAGTAC60400.029.0392511
GCAGAGT61550.028.5521289
TCTATCG500.00871173126.9350131
ACTTACC500.009216418526.6257383
CAGAGTA66800.026.4563410
AGTTTAT6400.026.00422
Produced by FastQC (version 0.11.5)