FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1105318
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54170.49008520624833757No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46550.4211457698146597No Hit
GTAATACAGAGCCGAGTCTTCGACTCTCAGGCTGTTCATTTGCAGATACA44930.40648935419490134No Hit
CAGTAATAGACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA38810.35112067296470334No Hit
CTATTACTGCATGCAAGCTCTGCAATCTCCGTGGACGTTCGGCCAAGGGA38460.34795416341722474No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35380.32008887939941266No Hit
GTATTACTGTGCGACAACTGGAGCGGTTCGGGCCGGACGCAATCACTACC33970.3073323695081415No Hit
GTAATAGACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA33020.298737557879271No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTC32500.2940330294087312No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32190.2912284066666787No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30960.28010038739982523No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30630.2771148212550596No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30300.27412925511029407No Hit
CTGTATTACTGTGCGACAACTGGAGCGGTTCGGGCCGGACGCAATCACTACCAGTTCGGTGTCGACGTCTGGGGC29540.26725340580719753No Hit
CAGTAATACAGAGCCGAGTCTTCGACTCTCAGGCTGTTCATTTGCAGATA29260.26472019816921466No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG28540.25820623567154427No Hit
GTCTATTACTGCATGCAAGCTCTGCAATCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA28090.25413500911050035No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27670.2503351976535259No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27070.24490689557213396No Hit
GGTCTAGTCAGAGCCTCCTCCATAGTAATGGATACAACTATTTGGATTGGTATCTGCAGAAGCCAGGGCAGTCTC26760.24210227283008148No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26340.23830246137310712No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT26320.23812151797039405No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25720.23269321588900208No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25290.22880293273067118No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25120.22726491380761013No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC23900.2162273662421131No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22920.20736113950917293No Hit
GATTTTACACTGAAAATCAGTAGAGTGGAGGCTGAGGATGTTGGGGTCTATTACTGCATGCAAGCTCTGCAATCT22650.20491840357254654No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22400.2026566110386332No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGGAGATTG21650.19587123343689328No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21590.19532840322875408No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGAGGGGATATTGTGATGA21550.19496651642332796No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTACTTGGGTTCTAATCGGGCC20690.18718595010666614No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT20450.18501462927410936No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG20430.18483368587139629No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20270.18338613864969175No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA20250.1832051952469787No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGAGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC20170.18248142163612643No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG19020.17207717598012517No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18760.16972491174485532No Hit
AGCCTGGAGGGTCCCTGAGACTCTCCTGTGTCGCCTCTGGAATCCCCTTC18730.16945349664078574No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAGTAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGATA18520.16755359091229854No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18290.1654727417810983No Hit
GTAATGGATACAACTATTTGGATTGGTATCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTACTTGGGTT17980.16266811903904577No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17720.16031585480377591No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGAGGGGATATTGTGATGACTCAGTCTCCACTCTCC17490.15823500567257567No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG17380.15723981695765382No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17300.15651604334680155No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17230.15588274143730582No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16890.15280670359118373No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16480.14909736383556588No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16290.14737840150979176No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA16200.14656415619758295No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGTAGAG15900.14385000515688698No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGAGGGGATATTGTGATGACTCAGTCTCCACT15860.14348811835146086No Hit
CTGCAATCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACG15610.14122632581754754No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15600.14113585411619098No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC15600.14113585411619098No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15390.1392359483877038No Hit
GGATACAACTATTTGGATTGGTATCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTACTTGGGTTCTAAT15330.13869311817956462No Hit
CCTCCATAGTAATGGATACAACTATTTGGATTGGTATCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTA15310.13851217477685154No Hit
CTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC15080.1364313256456513No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14830.134169533111738No Hit
CTCCTGTGTCGCCTCTGGAATCCCCTTCAATACCTATGGCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG14540.13154585377239852No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14380.13009830655069402No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14270.12910311783577214No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC14260.12901264613441563No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT14100.1275650989127111No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14050.12711274040592843No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13820.1250318912747282No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13800.1248509478720151No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13600.12304151384488445No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13290.12023689110283195No Hit
GCCCTGGCTTCTGCAGATACCAATCCAAATAGTTGTATCCATTACTATGG13250.1198750042974058No Hit
CCATAGTAATGGATACAACTATTTGGATTGGTATCTGCAGAAGCCAGGGC13170.11915123068655356No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13090.11842745707570129No Hit
GGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGG13080.11833698537434476No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13020.11779415516620557No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGG12840.11616566454178798No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12790.11571330603500532No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12780.11562283433364877No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12600.11399434370923119No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12560.11363245690380507No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12550.11354198520244851No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12550.11354198520244851No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12510.1131800983970224No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12500.11308962669566586No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12430.11245632478617013No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA12380.11200396627938747No Hit
AGTCAGAGCCTCCTCCATAGTAATGGATACAACTATTTGGATTGGTATCTGCAGAAGCCAGGGCAGTCTCCACAG12370.11191349457803094No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12320.11146113607124829No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12280.11109924926582214No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT12190.11028500395361335No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12080.10928981523869148No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC12070.10919934353733496No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11990.1084755699264827No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11960.10820415482241309No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11920.10784226801698697No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC11890.10757085291291738No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11890.10757085291291738No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT11870.10738990951020431No Hit
GTAGAGTGGAGGCTGAGGATGTTGGGGTCTATTACTGCATGCAAGCTCTG11840.1071184944061347No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11620.105128116976291No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11420.10331868294916034No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11400.10313773954644725No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11390.10304726784509073No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGAGGGGATATTGTGATGACTCAGTCTC11320.10241396593559501No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11310.10232349423423848No Hit
CCATGGAACTGGGGCTCCGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGT11280.10205207913016887No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11260.1018711357274558No Hit
CTCATACATTACGCAGACTCAGTGAAGGGGCGATTCACCGTCTCCAGAGACAACGCCAAGGACTCACTGTATCTG11260.1018711357274558No Hit
GCCTTAGCCCTGGATTCCAAGGCCTATCCACTTGGTGATCAGCACTGAGC11200.1013283055193166No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11160.10096641871389048No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)