FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1105318
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53340.48257605503574535No Hit
GTAATACAGAGCCGAGTCTTCGACTCTCAGGCTGTTCATTTGCAGATACA41950.37952878719065464No Hit
CAGTAATAGACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA38250.34605425768873754No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36650.33157878547169234No Hit
CTATTACTGCATGCAAGCTCTGCAATCTCCGTGGACGTTCGGCCAAGGGA35660.3226220870373956No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34980.31647001134515135No Hit
GTATTACTGTGCGACAACTGGAGCGGTTCGGGCCGGACGCAATCACTACC34260.309956048847481No Hit
GTAATAGACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA33440.3025373693362453No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33420.30235642593353224No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32290.2921331236802441No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30800.2786528401781207No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30030.27168651917366765No Hit
CTGTATTACTGTGCGACAACTGGAGCGGTTCGGGCCGGACGCAATCACTACCAGTTCGGTGTCGACGTCTGGGGC28970.26209651882987517No Hit
CAGTAATACAGAGCCGAGTCTTCGACTCTCAGGCTGTTCATTTGCAGATA27260.2466258578979081No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTC27210.24617349939112546No Hit
GTCTATTACTGCATGCAAGCTCTGCAATCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA27120.24535925407891665No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT26280.23775963116496793No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26120.2363120839432634No Hit
GGTCTAGTCAGAGCCTCCTCCATAGTAATGGATACAACTATTTGGATTGGTATCTGCAGAAGCCAGGGCAGTCTC25380.22961717804287998No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG24750.22391746085741843No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24410.2208414230112963No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23550.21306085669463448No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23500.2126084981878518No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC23460.21224661138242568No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23140.20935151693901666No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23010.20817538482138173No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22960.20772302631459905No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22800.2062754790928945No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22450.20310896954541588No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21420.193790384305693No Hit
GATTTTACACTGAAAATCAGTAGAGTGGAGGCTGAGGATGTTGGGGTCTATTACTGCATGCAAGCTCTGCAATCT21190.19170953517449277No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20130.1821195348307003No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19710.17831972337372595No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT19510.1765102893465953No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGGAGATTG19350.17506274212489076No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG19220.17388661000725583No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG19140.17316283639640356No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTACTTGGGTTCTAATCGGGCC19080.17262000618826437No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA19070.17252953448690783No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17720.16031585480377591No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGAGGGGATATTGTGATGA17720.16031585480377591No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA17320.1566969867495146No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGAGGGGATATTGTGATGACTCAGTCTCCACTCTCC17280.15633509994408848No Hit
GTAATGGATACAACTATTTGGATTGGTATCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTACTTGGGTT17060.15434472251424478No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGAGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC16760.1516305714735488No Hit
AGCCTGGAGGGTCCCTGAGACTCTCCTGTGTCGCCTCTGGAATCCCCTTC16720.15126868466812265No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16430.14864500532878322No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAGTAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGATA16370.148102175120644No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGTAGAG16200.14656415619758295No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG15960.14439283536502617No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15870.14357859005281737No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15720.14222151453246937No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15660.1416786843243302No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15600.14113585411619098No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15370.13905500498499074No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15360.13896453328363423No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGAGGGGATATTGTGATGACTCAGTCTCCACT15070.13634085394429477No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA14820.13407906141038145No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14760.13353623120224226No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC14690.13290292929274652No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14410.1303697216547636No Hit
CTCCTGTGTCGCCTCTGGAATCCCCTTCAATACCTATGGCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG14380.13009830655069402No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14220.1286507593289895No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14070.1272936838086415No Hit
GGATACAACTATTTGGATTGGTATCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTACTTGGGTTCTAAT13890.12566519318422392No Hit
GGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGG13880.12557472148286736No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13880.12557472148286736No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13860.1253937780801543No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13750.12439858936523245No Hit
CTGCAATCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACG13730.12421764596251939No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13730.12421764596251939No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13590.12295104214352794No Hit
CTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC13460.121774910025893No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT13410.12132255151911034No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC13320.12050830620690155No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13260.11996547599876235No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13010.11770368346484902No Hit
CCTCCATAGTAATGGATACAACTATTTGGATTGGTATCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTA12920.11688943815264025No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12920.11688943815264025No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12640.11435623051465732No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT12580.11381340030651813No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12560.11363245690380507No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12470.11281821159159627No Hit
GCCTTAGCCCTGGATTCCAAGGCCTATCCACTTGGTGATCAGCACTGAGC12470.11281821159159627No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12430.11245632478617013No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12400.11218490968210053No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGG12340.11164207947396135No Hit
GCCCTGGCTTCTGCAGATACCAATCCAAATAGTTGTATCCATTACTATGG12260.11091830586310908No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12120.10965170204411763No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12110.10956123034276109No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12070.10919934353733496No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11710.10594236228849978No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11700.10585189058714324No Hit
CCATAGTAATGGATACAACTATTTGGATTGGTATCTGCAGAAGCCAGGGC11500.10404245656001258No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC11420.10331868294916034No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGAGGGGATATTGTGATGACTCAGTCTC11420.10331868294916034No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC11340.10259490933830807No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11240.10169019232474275No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11220.10150924892202969No Hit
AGTCAGAGCCTCCTCCATAGTAATGGATACAACTATTTGGATTGGTATCTGCAGAAGCCAGGGCAGTCTCCACAG11190.10123783381796009No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)