FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences669638
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41990.6270552149071588No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36700.5480573085756781No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCGGAGTGTCT27010.4033522589817185No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26790.4000669018185945No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25780.38498412575152546No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24450.36512264835627606No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24280.36258396327568027No Hit
GTACATGAGCAACTTAGGAGGCTGTCCTGGCTTCTGCTGATACCAAGCTA24180.36109061911062396No Hit
ACTTAGGAGGCTGTCCTGGCTTCTGCTGATACCAAGCTAAGTAGTTCTTATTGTTGGTGAATAAGACACTCCGA23310.3480985248746338No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23150.3457091742105436No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21810.3256983623987886No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21300.31808230715700125No Hit
AGCCAGGACAGCCTCCTAAGTTGCTCATGTACTGGGCGTCTGCCCGGGACTCCGGGGTCCCTGACCGCTTCAGTG20790.31046625191521393No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20550.30688222591907865No Hit
GTCCCTGACCGCTTCAGTGCCAGCGGGTCTGGGACAGATTTCACTCTCAC20400.30464220967149414No Hit
GTCTTATTCACCAACAATAAGAACTACTTAGCTTGGTATCAGCAGAAGCC20390.30449287525498847No Hit
CTCCTAAGTTGCTCATGTACTGGGCGTCTGCCCGGGACTCCGGGGTCCCT20110.30031151159283076No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19980.2983701641782575No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19880.29687682001320115No Hit
CCCTGGCTCTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGT19060.2846313978597392No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC18740.2798526965315588No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18730.2797033621150532No Hit
GTGCAGCCACAGTTCGTTTGATCTCTACCTTGGTCCCTCCGCCGAAAGTG17750.26506858929750104No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17730.26476992046448977No Hit
GTCTCCAGACTCCCTGGCTCTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCGGAGTGTCTTATT17280.2580498717217362No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16210.24207108915563336No Hit
CTCATGTACTGGGCGTCTGCCCGGGACTCCGGGGTCCCTGACCGCTTCAG15980.23863639757600377No Hit
GTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGGCACTGAAGCG15850.23669505016143053No Hit
GTCCTGGCTTCTGCTGATACCAAGCTAAGTAGTTCTTATTGTTGGTGAAT15830.23639638132841923No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGTACTAAAATATTGTTGACAGTAATAAGTTGCCACATCTTCAGC15570.23251368649927273No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15520.23176701441674458No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15380.2296763325856657No Hit
GTAATAAGTTGCCACATCTTCAGCCTGCAGGGTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGGCA15370.22952699816916003No Hit
CTTCAGTGCCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCACCC15310.22863099167012624No Hit
ACTTATTACTGTCAACAATATTTTAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTAGAGATCAAACGAA15090.2253456345070023No Hit
GTCAGGGACCCCGGAGTCCCGGGCAGACGCCCAGTACATGAGCAACTTAGGAGGCTGTCCTGGCTTCTGCTGATA14820.2213136052613502No Hit
GACCAAGGTAGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT14790.22086560201183325No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14330.21399621885257405No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14260.21295087793703465No Hit
GCCCGGGACTCCGGGGTCCCTGACCGCTTCAGTGCCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCACC13810.20623082919428107No Hit
GTACTAAAATATTGTTGACAGTAATAAGTTGCCACATCTTCAGCCTGCAGGGTGCTGATGGTGAGAGTGAAAT13410.20025745253405572No Hit
GATTTCACTCTCACCATCAGCACCCTGCAGGCTGAAGATGTGGCAACTTATTACTGTCAACAATATTTTAGTACT13330.19906277720201063No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13260.19801743628647123No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13180.19682276095442613No Hit
GCTCATGTACTGGGCGTCTGCCCGGGACTCCGGGGTCCCTGACCGCTTCAGTGCCAGCGGGTCTGGGACAGATTT12900.19264139729226837No Hit
CCATCAGCACCCTGCAGGCTGAAGATGTGGCAACTTATTACTGTCAACAA12840.19174539079323455No Hit
ATTTTAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTAGAGATCAAA12800.191148053127212No Hit
CAATAAGAACTACTTAGCTTGGTATCAGCAGAAGCCAGGACAGCCTCCTAAGTTGCTCATGTACTGGGCGTCTGC12730.19010271221167258No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12500.186668020632043No Hit
GTCCCAGACCCGCTGGCACTGAAGCGGTCAGGGACCCCGGAGTCCCGGGC12480.18636935179903172No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCTACCTTGGTCC12280.18338266346891902No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12240.1827853258028965No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12160.1815906504708514No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12090.18054530955531198No Hit
GAGTGAAATCTGTCCCAGACCCGCTGGCACTGAAGCGGTCAGGGACCCCG12060.18009730630579507No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT11940.17830529330772746No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11410.17039056923292883No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11310.1688972250678725No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11240.16785188415233304No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTCTGTCTCTGGGCGAGAGGGCCACCATCAACTG11050.165014530238726No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTCTGTCTCTGGGCGAGAGGG10560.1576971438299499No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10550.1575478094134443No Hit
GTCCCGGGCAGACGCCCAGTACATGAGCAACTTAGGAGGCTGTCCTGGCTTCTGCTGATACCAAGCTAAGTAGTT10390.15515845874935413No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10150.15157443275321889No Hit
GTAATAGACGGCCGTGTCTGCGGCGGTCACAGAGGTCAGATTCAGGGAGA10120.151126429503702No Hit
GAACTACTTAGCTTGGTATCAGCAGAAGCCAGGACAGCCTCCTAAGTTGCTCATGTACTGGGCGTCTGCCCGGGA10020.14963308533864564No Hit
TCTTCAGCCTGCAGGGTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGGCACTGAAGCGGTCAGGGACC9950.1485877444231062No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9950.1485877444231062No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9920.14813974117358933No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9920.14813974117358933No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9900.14784107234057806No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9840.14694506584154424No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9610.14351037426191462No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG9520.14216636451336392No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9470.14141969243083577No Hit
GAATAAGACACTCCGACTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACAGAGCCAGGGAGTCTGGAGA9410.14052368593180195No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9330.1393290105997569No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9240.13798500085120616No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9130.13634232226964418No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9120.13619298785313858No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9100.1358943190201273No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9080.13559565018711603No Hit
GTACTGGGCGTCTGCCCGGGACTCCGGGGTCCCTGACCGCTTCAGTGCCAGCGGGTCTGGGACAGATTTCACTCT9080.13559565018711603No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9060.13529698135410476No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA8990.13425164043856533No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT8950.13365430277254278No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8910.13305696510652024No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC8830.13186228977447514No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8740.13051828002592444No Hit
CTACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGTACTAAAATATTGTTGACAGTAATAAGTTGCCACATCTTCAG8690.1297716079433963No Hit
CCCAGAGACAGAGCCAGGGAGTCTGGAGACTGGGTCATCACGATGTCCCC8590.12827826377833992No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8550.1276809261123174No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT8450.12618758194726107No Hit
CTGCAAGTCCAGTCGGAGTGTCTTATTCACCAACAATAAGAACTACTTAG8440.1260382475307554No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC8440.1260382475307554No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8310.12409690011618217No Hit
GCACTGAAGCGGTCAGGGACCCCGGAGTCCCGGGCAGACGCCCAGTACATGAGCAACTTAGGAGGCTGTCCTGG8290.1237982312831709No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT8280.12364889686666528No Hit
TTAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTAGAGATCAAACGA8270.12349956245015963No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC8270.12349956245015963No Hit
GGACTCCGGGGTCCCTGACCGCTTCAGTGCCAGCGGGTCTGGGACAGATT8240.12305155920064273No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA8170.1220062182851033No Hit
GTGCCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCACCCTGCAGGCTGAAGATGTGGCAACTTATTA8020.11976620203751878No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG7980.11916886437149624No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCACCCTGCAGGCTGAAGATG7920.11827285787246243No Hit
CAGTAATAAGTTGCCACATCTTCAGCCTGCAGGGTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGGCA7850.11722751695692299No Hit
CATCAGCACCCTGCAGGCTGAAGATGTGGCAACTTATTACTGTCAACAATATTTTAGTACTCCGCTCACTTTCGG7790.11633151045788918No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC7730.11543550395885536No Hit
CCAAGGTAGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT7520.11229948121223707No Hit
CTACTTAGCTTGGTATCAGCAGAAGCCAGGACAGCCTCCTAAGTTGCTCATGTACTGGGCGTCTGCCCGGGACTC7480.11170214354621452No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA7420.11080613704718072No Hit
CTCCCTGGCTCTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCA7410.1106568026306751No Hit
CTTATTACTGTCAACAATATTTTAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTAGAGATCAAACGAA7410.1106568026306751No Hit
AGGTAGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG7160.10692344221803422No Hit
GAGTACTAAAATATTGTTGACAGTAATAAGTTGCCACATCTTCAGCCTGCAGGGTGCTGATGGTGAGAGTGAAAT7100.1060274357190004No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7070.10557943246948351No Hit
CACCATCAGCACCCTGCAGGCTGAAGATGTGGCAACTTATTACTGTCAACAATATTTTAGTACTCCGCTCACTTT7040.10513142921996661No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG7040.10513142921996661No Hit
CTTTCGGCGGAGGGACCAAGGTAGAGATCAAACGAACTGTGGCTGCACCA6950.10378741947141588No Hit
ATATTTTAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTAGAGATCAAACGAACTGTGGCTGCACCAT6950.10378741947141588No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA6920.10333941622189899No Hit
ATCTCTACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGTACTAAAATATTG6910.10319008180539337No Hit
GTATCAGCAGAAGCCAGGACAGCCTCCTAAGTTGCTCATGTACTGGGCGTCTGCCCGGGACTCCGGGGTCCCTGA6910.10319008180539337No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT6890.1028914129723821No Hit
CTCCAGACTCCCTGGCTCTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCGGAGTGTCTTATTCA6840.10214474088985391No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT6810.10169673764033701No Hit
ACCATCAACTGCAAGTCCAGTCGGAGTGTCTTATTCACCAACAATAAGAACTACTTAGCTTGGTATCAGCAGAAG6710.10020339347528066No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCAAG200.00711481352.19111648
CGTACTA408.681018E-751.37862429
ACCGTAC408.73044E-751.33635327
ACGAGAC507.461858E-847.8566722
CGTATGC451.8379906E-646.08030341
GTACTAG451.951783E-645.6801430
GACCGTA451.9750441E-645.60162426
TACCGCA306.6125754E-445.5641677
CACGAGA551.578901E-743.50606521
GTGGTAT1200.042.7259751
GCTTGTA651.2343662E-842.0686531
TCGTATG503.8242106E-641.42534340
ACTAGAT504.002164E-641.15217632
CCGACCG857.2759576E-1140.20367410
GAATAAT350.00141040939.063751
TCAATAC350.001411963139.0553
ATATACT350.001411963139.0554
CGACCGG901.364242E-1037.97014211
GTATCAA14050.037.9516141
ATGCCGT557.1777795E-637.79625744
Produced by FastQC (version 0.11.5)