FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences817276
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCT13940.17056661397129No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC13430.1643263719967306No Hit
GTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA11340.13875361566961467No Hit
CTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG9720.11893167057395544No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT9270.1134255747140501No Hit
GCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGAC8470.10363695985199613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1700.046.60504540
CTCGTAT1550.044.30000739
CGTATGC1850.041.13278241
GGTATCA15250.039.9180531
GTATCAA36800.037.2652361
CCGTCTT2400.035.16088547
TATACAC3200.034.4846233
ATCTCGT2150.033.33719637
ACGAGAC2650.032.8187422
ATGCCGT2500.030.68492144
GCCGTCT2650.030.41718546
GTGGTAT3900.030.0172751
TCAACGC47350.029.237614
ATCAACG47550.029.1146343
ATCTCCG3000.028.97569710
CAACGCA48200.028.7913865
TTATACA3150.028.6625422
CGTCTTC3100.028.45258148
AACGCAG49150.028.1668536
TCTCCGA3100.028.04099811
Produced by FastQC (version 0.11.5)