FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences817276
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25150.3077295797258209No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC13160.1610227144807874No Hit
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCT12370.1513564573045091No Hit
GTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA10530.12884264312178506No Hit
CTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG10000.12235768577567431No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15950.037.961891
GTATCAA37800.037.435061
CCGTCAT1201.9995783E-532.54196570
ATCAACG47550.029.748173
TCAACGC47600.029.7169234
CAACGCA48150.029.4505335
CGCAGAG48600.028.9008128
CAACGCT1053.1224772E-828.6929923
AACGCAG49550.028.684176
CACGAGT500.006430568628.66288253
GCAGAGT50200.028.1760759
AGAGTAC49650.028.08582711
ACGCAGA51050.027.8413437
TATCAAC51750.027.5310542
CAGAGTA50600.027.49243710
CACGACA600.00917864926.64688768
CGAAGGA1507.159724E-826.3389969
GCGTAGA1259.6740434E-426.03357370
GTGGTAT5700.025.8230631
TGGTATC5900.024.9415972
Produced by FastQC (version 0.11.5)