FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1153200
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTGTGATGACTCAGCCACCTTCAGTATCTGGGACCC73720.6392646548733958No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC60000.5202913631633715No Hit
GCCTTGGGCTGACTCAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCCAGGCACGCAGACTGTCATCCCAGG51760.4488380159556018No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGATGACTCAGCCACCTTCAGTATCT50970.44198751300728406No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG48500.420568851890392No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT44250.38371488033298645No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA39840.34547346514047866No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT39220.34009712105445716No Hit
GTCTGTGATGACTCAGCCACCTTCAGTATCTGGGACCCCCGGACAGACGA39070.3387963926465487No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC37970.32925771765522027No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCACCTTGGTCCCTC37180.32240721470690253No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG34500.29916753381893857No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCTAAGTCTGGCGCCTCAGCCTCCCTGGCCATCAGAGGGCTCCGGT34360.2979535206382241No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT33600.29136316337148804No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGATGACTCAGCCAC33560.29101630246271243No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG32880.2851196670135276No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG30880.2677766215747485No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC29870.2590183836281651No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA29390.2548560527228581No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA29030.2517343045438779No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC28930.25086715227193895No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28340.24575095386749915No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG28080.24349635796045785No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT28060.24332292750607007No Hit
CCCCTGAGGACCTCAGACCATTTCTATAGATGAGGAGTTTGGGGGCCATGCCTGGAAGTTGTTGGTACCAATAAA27960.24245577523413112No Hit
ATAATAATCACCCTCATCCTCGGACCGGAGCCCTCTGATGGCCAGGGAGG27760.2407214706902532No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG27740.2405480402358654No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC26390.22884148456468958No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA25120.2178286507110649No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC24210.20993756503642041No Hit
GTTTGGGGGCCATGCCTGGAAGTTGTTGGTACCAATAAACATACTTAAATCCGCCTCCAAATCCGCCGCTGGAA23680.20534165799514395No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC23590.2045612209503989No Hit
CTGTCATCCCAGGCTGCACAATAATAATCACCCTCATCCTCGGACCGGAG23550.2042143600416233No Hit
GGTCTGAGGTCCTCAGGGGTCCCTGACCGATTCTCTGGCTCTAAGTCTGG23530.20404092958723552No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG23440.20326049254249046No Hit
GGATGACAGTCTGCGTGCCTGGGTTTTCGGCGGAGGGACCAAGGTGACCGTCCTGAGTCAGCCCAAGGCTGCCCC22840.19805757891085676No Hit
CCTTGGTCCCTCCGCCGAAAACCCAGGCACGCAGACTGTCATCCCAGGCTGCACAATAATAATCACCCTCATCCT22760.1973638570933056No Hit
GCCTGGGATGACAGTCTGCGTGCCTGGGTTTTCGGCGGAGGGACCAAGGTGACCGTCCTGAGTCAGCCCAAGG22680.19667013527575442No Hit
GTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC22390.19415539368713147No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22320.1935483870967742No Hit
GACTCAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCCAGGCACGCAGA22140.1919875130072841No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCACCTTGGTCCCTCCGCCGAAAAC22130.19190079778009017No Hit
GATTATTATTGTGCAGCCTGGGATGACAGTCTGCGTGCCTGGGTTTTCGG21870.1896462018730489No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA21610.18739160596600762No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC21470.18617759278529308No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT21450.1860041623309053No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT20690.17941380506416926No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20410.17698577870274018No Hit
TCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19990.1733437391605966No Hit
GGACCAAGGTGACCGTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG19700.17082899757197365No Hit
GGCCATCAGAGGGCTCCGGTCCGAGGATGAGGGTGATTATTATTGTGCAG19420.16840097121054456No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC19050.16519250780437045No Hit
CCTCAGACCATTTCTATAGATGAGGAGTTTGGGGGCCATGCCTGGAAGTT18890.16380506416926813No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT18580.16111689212625738No Hit
CTCTTGTTCTGGAAGCAGTTCCAGCGGCGGATTTGGAGGCGGATTTAAGT18500.1604231703087062No Hit
GTGATGACTCAGCCACCTTCAGTATCTGGGACCCCCGGACAGACGATTGA18320.15886229621921608No Hit
TTATTATTGTGCAGCCTGGGATGACAGTCTGCGTGCCTGGGTTTTCGGCG18260.1583420048560527No Hit
GATTTAAGTATGTTTATTGGTACCAACAACTTCCAGGCATGGCCCCCAAA18000.15608740894901144No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA17980.15591397849462366No Hit
GTCCTCAGGGGTCCCTGACCGATTCTCTGGCTCTAAGTCTGGCGCCTCAG17920.1553936871314603No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC17800.15435310440513353No Hit
GTATCTGGGACCCCCGGACAGACGATTGACGTCTCTTGTTCTGGAAGCAG17670.1532258064516129No Hit
CCCCCGGACAGACGATTGACGTCTCTTGTTCTGGAAGCAGTTCCAGCGGC17650.15305237599722513No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG17650.15305237599722513No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCACCTTGGTC17630.15287894554283732No Hit
ATCCCAGGCTGCACAATAATAATCACCCTCATCCTCGGACCGGAGCCCTC17470.151491501907735No Hit
CCTTCAGTATCTGGGACCCCCGGACAGACGATTGACGTCTCTTGTTCTGG17470.151491501907735No Hit
ACTCAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCCAGGCACGCAGAC17430.1511446409989594No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC17270.1497571973638571No Hit
GCACAATAATAATCACCCTCATCCTCGGACCGGAGCCCTCTGATGGCCAGGGAGGCTGAGGCGCCAGACTTAGAG16780.14550815123135621No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA16690.14472771418661115No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT16600.14394727714186611No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG16590.1438605619146722No Hit
TTTCTATAGATGAGGAGTTTGGGGGCCATGCCTGGAAGTTGTTGGTACCA16540.14342698577870272No Hit
TTATTGTGCAGCCTGGGATGACAGTCTGCGTGCCTGGGTTTTCGGCGGAG16500.14308012486992716No Hit
CTGATGGCCAGGGAGGCTGAGGCGCCAGACTTAGAGCCAGAGAATCGGTC16300.14134582032604925No Hit
CAATAATAATCACCCTCATCCTCGGACCGGAGCCCTCTGATGGCCAGGGA16280.14117238987166147No Hit
CTTCCAGGCATGGCCCCCAAACTCCTCATCTATAGAAATGGTCTGAGGTCCTCAGGGGTCCCTGACCGATTCT16260.14099895941727367No Hit
CCCTCATCCTCGGACCGGAGCCCTCTGATGGCCAGGGAGGCTGAGGCGCC16050.13917793964620187No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG15920.13805064169268125No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC15850.13744363510232396No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC15660.13579604578563995No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAG15620.13544918487686436No Hit
ATCTGGGACCCCCGGACAGACGATTGACGTCTCTTGTTCTGGAAGCAGTT15350.1331078737426292No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGATGACTCAGCCACCTTCAGTATCTGGGACCCCCGGACAGACGATTGA15330.13293444328824142No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG15200.13180714533472077No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC15090.13085327783558795No Hit
ATAGAAATGGTCTGAGGTCCTCAGGGGTCCCTGACCGATTCTCTGGCTCTAAGTCTGGCGCCTCAGCCTCCCTGG15060.13059313215400623No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG14890.12911897329171002No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA14830.12859868192854665No Hit
CTATAGAAATGGTCTGAGGTCCTCAGGGGTCCCTGACCGATTCTCTGGCTCTAAGTCTGGCGCCTCAGCCTCC14750.1279049601109955No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT14670.12721123829344433No Hit
GGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT14530.12599722511272982No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC14370.12460978147762747No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC14320.12417620534165799No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC14270.12374262920568851No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTG14170.12287547693374958No Hit
GTCCGAGGATGAGGGTGATTATTATTGTGCAGCCTGGGATGACAGTCTGCGTGCCTGGGTTTTCGGCGGAGGGAC14160.12278876170655566No Hit
TCCTCGGACCGGAGCCCTCTGATGGCCAGGGAGGCTGAGGCGCCAGACTTAGAGCCAGAGAATCGGTCAGGGACC14060.12192160943461672No Hit
CCCCCAAACTCCTCATCTATAGAAATGGTCTGAGGTCCTCAGGGGTCCCT14000.12140131807145335No Hit
GTCCCTGACCGATTCTCTGGCTCTAAGTCTGGCGCCTCAGCCTCCCTGGC13990.12131460284425945No Hit
GTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCT13960.12105445716267776No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTG13950.12096774193548387No Hit
GTCAGGGACCCCTGAGGACCTCAGACCATTTCTATAGATGAGGAGTTTGGGGGCCATGCCTGGAAGTTGTTGGTA13860.12018730489073881No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC13820.11984044398196322No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13770.11940686784599375No Hit
GACTTAGAGCCAGAGAATCGGTCAGGGACCCCTGAGGACCTCAGACCATT13770.11940686784599375No Hit
CCTGAGGACCTCAGACCATTTCTATAGATGAGGAGTTTGGGGGCCATGCC13680.11862643080124871No Hit
GGATGAGGGTGATTATTATTGTGCAGCCTGGGATGACAGTCTGCGTGCCT13590.11784599375650365No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGATGACTCAGCCACCTTCAGTATCTGGGA13530.11732570239334025No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC13470.1168054110301769No Hit
GAACAAGAGACGTCAATCGTCTGTCCGGGGGTCCCAGATACTGAAGGTGG13190.11437738466874782No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCAC13040.1130766562608394No Hit
CATCTATAGAAATGGTCTGAGGTCCTCAGGGGTCCCTGACCGATTCTCTGGCTCTAAGTCTGGCGCCTCAGCCTC12810.11108220603537981No Hit
GATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12750.11056191467221643No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG12740.11047519944502254No Hit
GATGAGGAGTTTGGGGGCCATGCCTGGAAGTTGTTGGTACCAATAAACAT12720.11030176899063475No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG12680.10995490808185919No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC12570.10900104058272633No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG12520.10856746444675686No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA12490.10830731876517517No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT12470.10813388831078738No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA12440.1078737426292057No Hit
GCCCCCAAACTCCTCATCTATAGAAATGGTCTGAGGTCCTCAGGGGTCCCTGACCGATTCTCTGGCTCTAAGT12410.107613596947624No Hit
GTTTATTGGTACCAACAACTTCCAGGCATGGCCCCCAAACTCCTCATCTA12340.10700659035726674No Hit
CTCCTCATCTATAGAAATGGTCTGAGGTCCTCAGGGGTCCCTGACCGATT12240.10613943808532778No Hit
GGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCT12230.10605272285813389No Hit
CTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCT12090.10483870967741934No Hit
GAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTG12060.10457856399583768No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC12010.1041449878598682No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCACCTTGGTCCCTCCGCCGAAAACC11980.10388484217828652No Hit
CTTCAGTATCTGGGACCCCCGGACAGACGATTGACGTCTCTTGTTCTGGA11930.10345126604231704No Hit
CAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGG11850.10275754422476586No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT11840.10267082899757198No Hit
GTTCTGGAAGCAGTTCCAGCGGCGGATTTGGAGGCGGATTTAAGTATGTT11640.10093652445369407No Hit
CATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCACCA11620.10076309399930627No Hit
GCCCAGGACCCTGCACAGTGAGTGAGGAGGGTGAGGAGGAGAGGGAAGCCGGCCATGCTGGAGATTGTCCTGAAT11570.1003295178633368No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)