FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1153200
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTGTGATGACTCAGCCACCTTCAGTATCTGGGACCC70990.6155913978494624No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC57330.4971383975026014No Hit
GCCTTGGGCTGACTCAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCCAGGCACGCAGACTGTCATCCCAGG51370.4454561220950399No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG51060.4427679500520291No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGATGACTCAGCCACCTTCAGTATCT50640.4391259105098856No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT41330.3583940339923691No Hit
GTCTGTGATGACTCAGCCACCTTCAGTATCTGGGACCCCCGGACAGACGA38220.33142559833506763No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC38120.3305584460631287No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG36880.3198057578910857No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT35890.31122095039889003No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG35370.3067117585848075No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA35280.3059313215400625No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT33940.29431148109608046No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCACCTTGGTCCCTC33680.2920568851890392No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA33600.29136316337148804No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGATGACTCAGCCAC32430.28121748178980227No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCTAAGTCTGGCGCCTCAGCCTCCCTGGCCATCAGAGGGCTCCGGT30720.2663891779396462No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29300.2540756156781131No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC28580.24783211932015264No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG28220.2447103711411724No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCACCTTGGTCCCTCCGCCGAAAAC28170.2442767950052029No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG28030.24306278182448837No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA27510.23855359001040582No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC26370.22866805411030175No Hit
CCCCTGAGGACCTCAGACCATTTCTATAGATGAGGAGTTTGGGGGCCATGCCTGGAAGTTGTTGGTACCAATAAA26170.22693374956642387No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG26110.2264134582032605No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25690.22277141866111688No Hit
ATAATAATCACCCTCATCCTCGGACCGGAGCCCTCTGATGGCCAGGGAGG25600.22199098161637182No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG25270.21912937911897332No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC24550.21288588276101283No Hit
GTTTGGGGGCCATGCCTGGAAGTTGTTGGTACCAATAAACATACTTAAATCCGCCTCCAAATCCGCCGCTGGAA24490.21236559139784947No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24370.2113250086715227No Hit
GGTCTGAGGTCCTCAGGGGTCCCTGACCGATTCTCTGGCTCTAAGTCTGG24130.2092438432188692No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC23480.20360735345126604No Hit
GGATGACAGTCTGCGTGCCTGGGTTTTCGGCGGAGGGACCAAGGTGACCGTCCTGAGTCAGCCCAAGGCTGCCCC23390.20282691640652098No Hit
CTGTCATCCCAGGCTGCACAATAATAATCACCCTCATCCTCGGACCGGAG23080.20013874436351026No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA23020.19961845300034683No Hit
GCCTGGGATGACAGTCTGCGTGCCTGGGTTTTCGGCGGAGGGACCAAGGTGACCGTCCTGAGTCAGCCCAAGG22370.19398196323274366No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22280.1932015261879986No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA22070.19138050641692683No Hit
CCTTGGTCCCTCCGCCGAAAACCCAGGCACGCAGACTGTCATCCCAGGCTGCACAATAATAATCACCCTCATCCT22010.19086021505376344No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21320.18487686437738468No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC21120.18314255983350677No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT20950.18166840097121056No Hit
GTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC20890.18114810960804717No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA20080.17412417620534165No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT20020.17360388484217829No Hit
GATTATTATTGTGCAGCCTGGGATGACAGTCTGCGTGCCTGGGTTTTCGG19210.16657995143947277No Hit
GGCCATCAGAGGGCTCCGGTCCGAGGATGAGGGTGATTATTATTGTGCAG18880.16371834894207424No Hit
GACTCAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCCAGGCACGCAGA18680.1619840443981963No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC18630.16155046826222685No Hit
CTCTTGTTCTGGAAGCAGTTCCAGCGGCGGATTTGGAGGCGGATTTAAGT18450.15998959417273673No Hit
TTATTATTGTGCAGCCTGGGATGACAGTCTGCGTGCCTGGGTTTTCGGCG18400.15955601803676725No Hit
CCTCAGACCATTTCTATAGATGAGGAGTTTGGGGGCCATGCCTGGAAGTT18310.15877558099202219No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT18210.15790842872008326No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18030.15634755463059313No Hit
GGACCAAGGTGACCGTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG17920.1553936871314603No Hit
GTCCTCAGGGGTCCCTGACCGATTCTCTGGCTCTAAGTCTGGCGCCTCAG17680.1533125216788068No Hit
GTATCTGGGACCCCCGGACAGACGATTGACGTCTCTTGTTCTGGAAGCAG17660.153139091224419No Hit
TCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG17210.14923690600069373No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA17200.1491501907734998No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT17000.14741588622962193No Hit
TTTCTATAGATGAGGAGTTTGGGGGCCATGCCTGGAAGTTGTTGGTACCA16950.14698231009365245No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCACCTTGGTC16900.14654873395768298No Hit
ACTCAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCCAGGCACGCAGAC16820.1458550121401318No Hit
GATTTAAGTATGTTTATTGGTACCAACAACTTCCAGGCATGGCCCCCAAA16820.1458550121401318No Hit
ATCCCAGGCTGCACAATAATAATCACCCTCATCCTCGGACCGGAGCCCTC16520.14325355532431494No Hit
GTGATGACTCAGCCACCTTCAGTATCTGGGACCCCCGGACAGACGATTGA16510.14316684009712105No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA16400.1422129725979882No Hit
CCTTCAGTATCTGGGACCCCCGGACAGACGATTGACGTCTCTTGTTCTGG16330.14160596600763095No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGATGACTCAGCCACCTTCAGTATCTGGGACCCCCGGACAGACGATTGA16190.1403919528269164No Hit
CTTCCAGGCATGGCCCCCAAACTCCTCATCTATAGAAATGGTCTGAGGTCCTCAGGGGTCCCTGACCGATTCT16140.13995837669094693No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCACCTTGGTCCCTCCGCCGAAAACC15960.1383975026014568No Hit
GCACAATAATAATCACCCTCATCCTCGGACCGGAGCCCTCTGATGGCCAGGGAGGCTGAGGCGCCAGACTTAGAG15950.13831078737426292No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC15790.1369233437391606No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC15590.1351890391952827No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTG15450.13397502601456815No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA15290.13258758237946583No Hit
CTGATGGCCAGGGAGGCTGAGGCGCCAGACTTAGAGCCAGAGAATCGGTC15280.13250086715227194No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC15130.1312001387443635No Hit
CAATAATAATCACCCTCATCCTCGGACCGGAGCCCTCTGATGGCCAGGGA14890.12911897329171002No Hit
GGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT14830.12859868192854665No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14820.12851196670135276No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG14780.12816510579257717No Hit
ATCTGGGACCCCCGGACAGACGATTGACGTCTCTTGTTCTGGAAGCAGTT14720.1276448144294138No Hit
TTATTGTGCAGCCTGGGATGACAGTCTGCGTGCCTGGGTTTTCGGCGGAG14710.12755809920221992No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAG14640.12695109261186263No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC14580.12643080124869926No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14430.12513007284079083No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC14240.12348248352410685No Hit
GTCCGAGGATGAGGGTGATTATTATTGTGCAGCCTGGGATGACAGTCTGCGTGCCTGGGTTTTCGGCGGAGGGAC14220.12330905306971905No Hit
CCCCCGGACAGACGATTGACGTCTCTTGTTCTGGAAGCAGTTCCAGCGGC14190.12304890738813737No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT14150.12270204647936178No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG14040.12174817898022892No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC13980.12122788761706556No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC13940.12088102670828997No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC13870.1202740201179327No Hit
ATAGAAATGGTCTGAGGTCCTCAGGGGTCCCTGACCGATTCTCTGGCTCTAAGTCTGGCGCCTCAGCCTCCCTGG13800.11966701352757544No Hit
GTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCT13780.11949358307318765No Hit
CCCTCATCCTCGGACCGGAGCCCTCTGATGGCCAGGGAGGCTGAGGCGCC13740.11914672216441206No Hit
GGATGAGGGTGATTATTATTGTGCAGCCTGGGATGACAGTCTGCGTGCCT13690.11871314602844259No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTG13690.11871314602844259No Hit
CTATAGAAATGGTCTGAGGTCCTCAGGGGTCCCTGACCGATTCTCTGGCTCTAAGTCTGGCGCCTCAGCCTCC13640.11827956989247312No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13350.11576482830385015No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC13190.11437738466874782No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG13180.11429066944155393No Hit
GACTTAGAGCCAGAGAATCGGTCAGGGACCCCTGAGGACCTCAGACCATT13150.11403052375997226No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC13110.11368366285119666No Hit
CTCCTCATCTATAGAAATGGTCTGAGGTCCTCAGGGGTCCCTGACCGATT13050.1131633714880333No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA12900.11186264308012488No Hit
GTCCCTGACCGATTCTCTGGCTCTAAGTCTGGCGCCTCAGCCTCCCTGGC12800.11099549080818591No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGATGACTCAGCCACCTTCAGTATCTGGGA12620.1094346167186958No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCAC12510.10848074921956297No Hit
GGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCT12460.10804717308359346No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG12450.10796045785639959No Hit
CCTGAGGACCTCAGACCATTTCTATAGATGAGGAGTTTGGGGGCCATGCC12430.10778702740201179No Hit
GTCAGGGACCCCTGAGGACCTCAGACCATTTCTATAGATGAGGAGTTTGGGGGCCATGCCTGGAAGTTGTTGGTA12370.10726673603884843No Hit
CCCCCAAACTCCTCATCTATAGAAATGGTCTGAGGTCCTCAGGGGTCCCT12160.10544571626777662No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT12140.10527228581338882No Hit
GATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12100.10492542490461326No Hit
TCCTCGGACCGGAGCCCTCTGATGGCCAGGGAGGCTGAGGCGCCAGACTTAGAGCCAGAGAATCGGTCAGGGACC12090.10483870967741934No Hit
GTTTATTGGTACCAACAACTTCCAGGCATGGCCCCCAAACTCCTCATCTA12060.10457856399583768No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG12010.1041449878598682No Hit
CTTCAGTATCTGGGACCCCCGGACAGACGATTGACGTCTCTTGTTCTGGA11970.10379812695109261No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA11930.10345126604231704No Hit
GATGAGGAGTTTGGGGGCCATGCCTGGAAGTTGTTGGTACCAATAAACAT11920.10336455081512314No Hit
GCCCCCAAACTCCTCATCTATAGAAATGGTCTGAGGTCCTCAGGGGTCCCTGACCGATTCTCTGGCTCTAAGT11880.10301768990634756No Hit
CATCTATAGAAATGGTCTGAGGTCCTCAGGGGTCCCTGACCGATTCTCTGGCTCTAAGTCTGGCGCCTCAGCCTC11760.10197710718002082No Hit
CTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCT11720.10163024627124523No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC11650.10102323968088796No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)