FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169309
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT28161.6632311336077823No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGT4590.27110194968962076No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4000.23625442238746908No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3980.23507315027553174No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC3950.2333012421076257No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3120.18427844946222585No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3050.18014399707044518No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2760.16301555144735366No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2590.15297473849588622No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2380.1405713813205441No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2370.13998074526457543No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT2370.13998074526457543No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2360.13939010920860673No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2180.12875866020117063No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2120.1252148438653586No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2040.12048975541760923No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2000.11812721119373454No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1900.1122208506340478No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1800.10631449007436108No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1710.10099876557064302No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1710.10099876557064302No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG4600.0107.81093670
GTGAGGA350.001975086872.0479870
CGCTTAA200.002292316969.4315861
TCGCGTA150.002349648469.01253538
AAGGGGG4550.067.95080669
CTAGGGT150.00430025859.25380725
ATAGGGG351.8982009E-458.62289468
CCTAGGT150.00451286258.53632712
AGTATTG201.9162573E-458.5363275
GCCTAGG150.00451286258.53632711
CTAGGTT150.00451286258.53632713
TCACAAC250.004528808458.4672666
AAAAGGG4750.058.2432967
AAAAAGG4700.058.05259366
CTTAACT250.005014628656.98246463
AAAGGGG5050.056.8816268
CTTGAAA5650.056.83235557
TTGAAAA5950.056.76415358
TGCTTGA5550.055.4346955
TCTGCTT5650.053.4486153
Produced by FastQC (version 0.11.5)